Volume 36 Issue 3
May  2015
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Wen-Bin JIANG, Ma HAKIM, Lei LUO, Bo-Wen LI, Shi-Long YANG, Yu-Zhu SONG, Ren LAI, Qiu-Min LU. Purification and characterization of cholecystokinin from the skin of salamander Tylototriton verrucosus. Zoological Research, 2015, 36(3): 174-177.
Citation: Wen-Bin JIANG, Ma HAKIM, Lei LUO, Bo-Wen LI, Shi-Long YANG, Yu-Zhu SONG, Ren LAI, Qiu-Min LU. Purification and characterization of cholecystokinin from the skin of salamander Tylototriton verrucosus. Zoological Research, 2015, 36(3): 174-177.

Purification and characterization of cholecystokinin from the skin of salamander Tylototriton verrucosus

Funds:  This work was supported by the National Basic Research Program of China (973 Program) (2013CB911300), National Natural Science Foundation of China (U1132601) and the Chinese Academy of Sciences (SAJC201308)
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  • Corresponding author: Ren LAI, Qiu-Min LU
  • Received Date: 2015-03-16
  • Rev Recd Date: 2015-04-09
  • Publish Date: 2015-05-08
  • As a group of intestinal hormones and neurotransmitters, cholecystokinins (CCKs) regulate and affect pancreatic enzyme secretion, gastrointestinal motility, pain hypersensitivity, digestion and satiety, and generally contain a DYMGWMDFG sequence at the C-terminus. Many CCKs have been reported in mammals. However, only a few have been reported in amphibians, such as Hyla nigrovittata, Xenopus laevis, and Rana catesbeiana, with none reported in urodele amphibians like newts and salamanders. Here, a CCK called CCK-TV was identified and characterized from the skin of the salamander Tylototriton verrucosus. This CCK contained an amino acid sequence of DYMGWMDF-NH2 as seen in other CCKs. A cDNA encoding the CCK precursor containing 129 amino acid residues was cloned from the cDNA library of T. verrucosus skin. The CCK-TV had the potential to induce the contraction of smooth muscle strips isolated from porcine gallbladder, eliciting contraction at a concentration of 5.0x10-11 mol/L and inducing maximal contraction at a concentration of 2.0x10-6 mol/L. The EC50 was 13.6 nmol/L. To the best of our knowledge, this is the first report to identify the presence of a CCK in an urodele amphibian.
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