Volume 27 Issue 3
May  2006
Turn off MathJax
Article Contents
DUO Shu-guang, WU Ying-ji *, LUO Fen-hua, BOU Shorgan. Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells. Zoological Research, 2006, 27(3): 299-305.
Citation: DUO Shu-guang, WU Ying-ji *, LUO Fen-hua, BOU Shorgan. Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells. Zoological Research, 2006, 27(3): 299-305.

Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells

  • Received Date: 2006-12-03
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2006-06-22
  • Bovine mammary epithelial cells were isolated and purified by using collagenase digestion of the mammary gland tissue for the primary cell culture, and subsequently using trypsin-selected digestion of the cultured cells for cell purification. Morphological observation revealed that the cultured cells possessed the typical character of epithelial cells. Karyotyping analysis showed the normal chromosome number in cultured cells. The tissue-specific expression of cytokeratin 5 and 8 genes in mammary epithelial cells was identified by immunofluorescent cytochemical staining. The transcription of the betacasein gene was detected by RT-PCR, when the purified cells were induced with insulin, hydrocortisone and prolactin in the culture medium without serum. The above results indicate that the purified cells are mammary epithelial cells, which can transcript beta-casein mRNA in the induction condition.
  • loading
  • Relative Articles

    [1] Gang LIU, Daorina, Rong-Rong WANG, Yun-Xia LI, Yan-Feng DAI, Xi-He LI, Yu LI, Yao LI. Establishment of cell culture system of rough-legged buzzard and biological characteristic analysis on different tissue cells cultured in vitro. Zoological Research, 2013, 34(3): 244-248.  doi: 10.11813/j.issn.0254-5853.2013.3.0244
    [2] WANG Jin-Huan, SU Rong, SU Wei-Ting, CHENG Cheng, WEI Shu-Guang, DING Hai-Hua, DUAN Ying, LI Sheng-Bin, NIE Wen-Hui. Establishment of cell lines derived from skin biopsies of crested ibis (Nipponia nippon) and their biological characteristics. Zoological Research, 2012, 33(6): 591-596.  doi: 10.3724/SP.J.1141.2012.06591
    [3] CHEN Dong-liang, LI Rong-rong, ZHANG Jing, LU Bin, WEI Qiang, WANG Shu-fen, XIE Yun-h. Comparative Analysis of Five Different Homologous Feeder Cell Lines in the Ability to Support Rhesus Embryonic Stem Cells. Zoological Research, 2009, 30(4): 345-353.  doi: 10.3724/SP.J.1141.2009.04345
    [4] YI Bin, CHANG Hong, CAO Yi. Improvement of Methods for Total RNA Extraction from Hepatocarcinoma Tissues and Cell Lines and Comparison of Reverse Transcription and cDNA Cloning Strategies. Zoological Research, 2009, 30(5): 520-526.  doi: 10.3724/SP.J.1141.2009.05520
    [5] BAI Yin-ba-tu, LUO Fen-hua, HU Tian-yuan, HOU Yue, WU Ying-ji. Cloning of Sheep tnp2 Gene and Transcription Analysis of Round Spermatid Cells in the in vitro Co-culture System. Zoological Research, 2009, 30(3): 262-266.  doi: 10.3724/SP.J.1141.2009.03262
    [6] NIE Hong, YIN Zhen, LIN Qian-xuan, FENG Xue-ying, ZHANG Jian-yu, LI Ko-yan. Morphological Characteristics of Smooth Muscle Cells Isolated from the Rat Ductus Deferens. Zoological Research, 2008, 29(6): 633-636.  doi: 10.3724/SP.J.1141.2008.06633
    [7] SHI Hai-jing, ZHU Zheng, LIU Long-ding, HU Yun-zhang, *. Morphological Properties of Mesenchymal Stem Cells Derived from Bone Marrow of Rhesus Monkeys (in English). Zoological Research, 2007, 28(2): 213-216.
    [8] YANG Dong-shan * DU Chen-guang GAO Fei BOU Shorgan. In Vitro Culture of Bovine Fetal Fibroblast Cells Using Tissue Explant Attachment and Gene Transfection Through Electroporation. Zoological Research, 2006, 27(1): 41-47.
    [9] WANG Hong, JI Wei-Zhi. Comparison of Bovine Embryo Development In vitro in Brl/Mtcm199 and Hecm-6/mTcm199 Culture Media. Zoological Research, 2000, 21(6): 446-451.
    [10] ZHENG De-shu. Apoptosis and Programmed Cell Death. Zoological Research, 2000, 21(1): 17-22.
    [11] LI Jing-yan. Diplomonads and The Exploration on The Origin of Cell Nucleus. Zoological Research, 1996, 17(3): 275-286.
    [12] WANG Jin-xing, ZHAO Xiao-fan, WANG Xiang-min, TIAN Ming-cheng. Karyotype Analysis For Seven Species of Clupeiform and Perciform Fishes. Zoological Research, 1994, 15(2): 76-79.
    [13] LI Jing-yan. The Proposal of Evolutionary Cell Blology and Its Role. Zoological Research, 1989, 10(4): 319-326.
    [14] YAN Ju, LIU Shi-hus, ZHANG Xi-yuan, ZHANG Mu-xian. The application of cell synchronization to chromosome banding of fish and The tentative karyotype analysis of Ctenopharyngodon idellus. Zoological Research, 1989, 10(2): 123-128.
    [15] ZHU Su-tian, LU Qun, XIANG Wei. Establishment of Avian Feather Pulp Culture Method and Study on the Cell Division. Zoological Research, 1987, 8(1): 21-26.
    [16] WANG Gui-ying, HU Jie-yu, YAN Yu-juan. Action of Cytochalasin D on Cells of Mouse Mammary Adenocarcinoma Cell Line (MA782/5S-8102). Zoological Research, 1986, 7(1): 39-45.
    [17] YE Yin-ying, MAO Yi-ping, LU Xiao-xuan, WANG Shi-juan. The Observation of Biological Characteristics of Hybrid Cell in Mice. Zoological Research, 1984, 5(4): 319-324.
    [18] LI Ling-yan. Cell Theory. Zoological Research, 1982, 3(zk): 373-374.
    [19] XUE Kai-xian. Experimental studies on Cell Growth and cell Division in Amoeba discoides. Zoological Research, 1980, 1(1): 65-74.
    [20] LIU Rui-qing, GUO Jian-min, ZHANG Yao-ping, DIN Lin-hua. Karyotype Analysis of The Mouse Ascites Hepatoma. Zoological Research, 1980, 1(1): 82-90.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2144) PDF downloads(2532) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return