Volume 27 Issue 1
Jan.  2006
Turn off MathJax
Article Contents
ZHU Xiao-chen LIU Hai-jin , * SUN Xiao-wen XUE Ling-ling MAO Lian-ju. Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus). Zoological Research, 2006, 27(1): 63-67.
Citation: ZHU Xiao-chen LIU Hai-jin , * SUN Xiao-wen XUE Ling-ling MAO Lian-ju. Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus). Zoological Research, 2006, 27(1): 63-67.

Assessment of Homozygosity in Gynogenetic Diploid Using Microsatellite Markers in Japanese Flounder (Paralichthys olivaceus)

  • Received Date: 2005-11-07
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2006-02-22
  • Gynogenesis was thought to be a useful method to generate inbred lines in fish. In this study, analysis of 5-8 heterozygous loci in Japanese flounder (Paralichthys olivaceus) was used to measure the increment of homozygosity in meiogynogenetic diploid (Meiotic-A & Meiotic-B) and mitogynogenetic diploid (Mitotic-A). The result showed all homozygosity in Mitotic-G1, while a high percentage of heterozygosity in Meiosis-A and Meiosis-B except Poli11TUF. The rate of heterozygosity in meiogynogenetic progenies at the locus of Poli9TUF, Poli9-8TUF, Poli11TUF, Poli13TUF, Poli23TUF, Poli30TUF, Poli123TUF and Poli130TUF were 1.000 0, 1.000 0, 0.194 4, 0.945 9, 0.861 1, 1.000 0, 0.777 8 and 0.800 0, respectively. The average recombination rate of these eight loci is 0.822 4. The high proportion of heterozygotes for seven loci demonstrates that it is not a practical method for producing homozygous inbred lines in the gynogenetic fish produced by retention the second polar body; and treatments of suppressing the first cell division were more promising for this purpose.
  • loading
  • Relative Articles

    [1] Chao-Hao Chen, Bi-Jun Li, Xiao-Hui Gu, Hao-Ran Lin, Jun-Hong Xia. Marker-assisted selection of YY supermales from a genetically improved farmed tilapia-derived strain. Zoological Research, 2019, 40(2): 108-112.  doi: 10.24272/j.issn.2095-8137.2018.071
    [2] Jing Yang, Guo-Fen Zhu, Jian Jiang, Chang-Lin Xiang, Fu-Li Gao, Wei-Dong Bao. Non-invasive genetic analysis indicates low population connectivity in vulnerable Chinese gorals: concerns for segregated population management. Zoological Research, 2019, 40(5): 439-448.  doi: 10.24272/j.issn.2095-8137.2019.058
    [3] Chun-Lei WANG, Zhi-Peng WANG, Jia-Qi WANG, Ming-You Li, Xiao-Wu CHEN. Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder). Zoological Research, 2016, 37(5): 301-306.  doi: 10.13918/j.issn.2095-8137.2016.5.301
    [4] Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312.  doi: 10.13918/j.issn.2095-8137.2014.4.307
    [5] Ji-Min MA, You-Yi KUANG, Xian-Hu ZHENG, Ding-Chen CAO, Wei-Hua LYU, Yu CHANG, Dan ZHOU, Ke DU, Xiao-Wen SUN. Screening and body correlation analysis of microsatellite markers related to intermuscular bone number in common carp (Cyprinus carpio). Zoological Research, 2013, 34(4): 406-410.  doi: 10.11813/j.issn.0254-5853.2013.4.0406
    [6] Min TAO, Zhen-Yan SONG, Jun XIAO, Shao-Jun LIU, Kai-Kun LUO, Tuo-Mi ZOU, Jun WANG, Wei LIU, Jie HU, Ru-Rong ZHAO, Yun LIU. Cytogenetic and molecular genetic analysis of gynogenesis in Megalobrama amblycephala using spermatozoa of Erythroculter ilishaeformis. Zoological Research, 2013, 34(5): 479-486.  doi: 10.11813/j.issn.0254-5853.2013.5.0479
    [7] ZHOU Lu, WANG Cheng-Hui, CHENG Qi-Xuan, WANG Zhong-Qing. Comparison and analysis between PST and FST of mitten crabs in the Minjiang River. Zoological Research, 2012, 33(3): 314-318.  doi: 10.3724/SP.J.1141.2012.03314
    [8] QU Wen-Hui, LI Feng, SHA Jian-Bin, ZHANG Yu-Ming. Analyzing Japanese marsh warbler (Megalurus pryeri) song behavior in the breeding season. Zoological Research, 2011, 32(2): 141-149.  doi: 10.3724/SP.J.1141.2011.02141
    [9] CHEN Yan-Mei, QIN Dong-Dong, JIANG Hui-Hui, HU Xin-Tian, MA Yuan-Ye. Sleep disorder, a potential early diagnostic marker for psychiatric and neurodegenerative diseases. Zoological Research, 2011, 32(1): 81-90.  doi: 10.3724/SP.J.1141.2011.01081
    [10] JI Xu, SUN Xiao-wen, YANG Li-geng, Li Ya-juan, LIU Hai-jin. Paternity Testing of Mitogynogenetic Japanese Flounder (Paralichthys olivaceus) Using Microsatellite Loci. Zoological Research, 2008, 29(1): 25-30.
    [11] SHI Xian-wei, WEI Lin, HAN Hong-jin. Bi-PASA Genetic Marker and Polymorphism of the Porcine STCH Gene. Zoological Research, 2005, 26(1): 76-81.
    [12] XU Qing-gang, CHEN Ke-ping, YAO Qin, LIU Xiao-yong. Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm,Bombyx mori. Zoological Research, 2004, 25(1): 69-72.
    [13] ZHAO Jun, CHEN Xiang-lin. Phenotypic Differentiation of Offspring Developed from Pengze Crucian Carp Eggs Activated by Heterlogous Sperm. Zoological Research, 2003, 24(4): 297-301.
    [14] LIU Jing-xia, ZHOU Li, ZHAO Zhen-shan, GUI Jian-fang. Studies on Microsatellite Markers of Four Artificially Gynogenetic Families in Ornamental Carp. Zoological Research, 2002, 23(2): 97-105.
    [15] ZHOU Li, LIU Jin-Xia, GUI Jian-Fang. Preliminary Investigation on Genetic Diversity of Gynogenetic Silver Crucian Carp (Carassius auratus gibelio Bloch) Detected by Microsatellite DNA. Zoological Research, 2001, 22(4): 257-264.
    [16] ZHANG Mei-fen, WU Mei-xi, DING Han-bo. Study on The Development of Gynogenesis Haploid of Misgurnus anguillicaudatus. Zoological Research, 1993, 14(4): 361-366.
    [17] LI Bo, XIE Yu-hao, LU Yi-xin. The Study on Daily Otolith Rings of Young Japanese Eel (Anguilla japonica). Zoological Research, 1992, 13(3): 201-207.
    [18] LI Fu-lai, QIN Zai-xian, TEN Huai-mei. Study on The Microstructure and Components of Eggshell of Japanese Crested Ibis. Zoological Research, 1990, 11(3): 173-177.
    [19] ZHANG Ying-pei, LIU Hong, LOU Yun-dong. Electrophoresis of Isozymes and Muscle Protein of An Abnormal Crucian Carp. Zoological Research, 1989, 10(3): 249-250.
    [20] CUI Yue-li, ZAN Rui-guang. Studies on Gynogenesis of the High-Backed Form of Carassius auratus in Kunming Lake. Zoological Research, 1982, 3(zk): 237-242.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2487) PDF downloads(2343) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint