Volume 26 Issue 5
Sep.  2005
Turn off MathJax
Article Contents
CHENG Guo-feng, LIN Jiao-jiao, SUN Jun, LI Hao, ZHU Chuan-gang, ZHOU Yuan-cong, CAI You-min. Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum. Zoological Research, 2005, 26(5): 513-517.
Citation: CHENG Guo-feng, LIN Jiao-jiao, SUN Jun, LI Hao, ZHU Chuan-gang, ZHOU Yuan-cong, CAI You-min. Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum. Zoological Research, 2005, 26(5): 513-517.

Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2005-10-22
  • The expressed sequence tag of eukaryotic translation initiation factor 5 (eIF5) from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioinformatics method. The overlapping sequences were assembled into one that includes the complete open reading frame (GenBank accession number: AY686501). The full-length cDNA of SjeIF5 was cloned into a pET-28c(+) vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in Escherichia coli. The recombinant expression of eIF5 protein of Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.
  • loading
  • Relative Articles

    [1] Guang-Qi GAO, Li-Shuang SONG, Bin TONG, Guang-Peng LI. Expression levels of GSTA2 and APOD genes might be associated with carotenoid coloration in golden pheasant (Chrysolophus pictus) plumage. Zoological Research, 2016, 37(3): 144-150.  doi: 10.13918/j.issn.2095-8137.2016.3.144
    [2] Jin-Hua WANG, Liang MIAO, Ming-Yun LI, Xiao-Fei GUO, Na PAN, Ying-Ying CHEN, Liang ZHAO. Cloning the Dmrt1 and DmrtA2 genes of ayu (Plecoglossus altivelis) and mapping their expression in adult, larval, and embryonic stages. Zoological Research, 2014, 35(2): 99-107.  doi: 10.11813/j.issn.0254-5853.2014.2.099
    [3] Hong-Mei ZHANG, Yan-Fang SU, Zhi-Yi SHI, Yuan-Shuai FU. cDNA clone and expression analysis of α-Tropomyosin during Japanese flounder (Paralichthys olivaceus) metamorphosis. Zoological Research, 2014, 35(4): 307-312.  doi: 10.13918/j.issn.2095-8137.2014.4.307
    [4] WANG Fang, CHEN Jiong, SHI Yu-Hong, LU Xin-Jiang, LI Ming-Yun. Molecular cloning, sequence analysis and stress-related changes of the heat shock protein 60 gene in Neobenedenia melleni. Zoological Research, 2012, 33(6): 603-608.  doi: 10.3724/SP.J.1141.2012.06603
    [5] CHAO Yan, ZHAO Lan-Ying, LI Chang-Zhong, XIE Bao-Sheng, SHEN Zhi-Xin, WANG Guo-Jie, WANG Zhen-Gang, LI Chao, BAI Bin-Qiang, ZHANG Hui, QI De-Lin. cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi. Zoological Research, 2012, 33(5): 473-480.  doi: 10.3724/SP.J.1141.2012.05473
    [6] ZHANG Hao, FAN Chun-Xin, SONG Jia-Kun. Cloning and expression of Tbx3 gene in Siberian sturgeon, Acipenser baerii. Zoological Research, 2012, (2): 158-164.  doi: 10.3724/SP.J.1141.2012.02158
    [7] ZHUANG Yong-Hui, LI Si-Man, YU Guo-Yu, ZHANG Yong, XIANG Yang, ZOU Hao, LEE Wen-Hui. Bacterial expression and purification of biologically active human TFF2. Zoological Research, 2012, (2): 144-150.  doi: 10.3724/SP.J.1141.2012.02144
    [8] Jinling CAO, Jianjie CHEN, Zhongliang JIANG, Yongju LUO, Xi GAN. Molecularcloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus. Zoological Research, 2012, 33(E3-4): 40-46.  doi: 10.3724/SP.J.1141.2012.E03-04E40
    [9] FENG Li-Fang, CHANG Yue, YUAN Dong-Xia, MIAO Wei. Expression analysis of 5 hsp70 genes in Tetrahymena thermophila. Zoological Research, 2011, 32(3): 267-276.  doi: 10.3724/SP.J.1141.2011.03267
    [10] yu guo-Yu, XIANG Yang, ZHANG Hong-Yun, JIANG Ping, Lee Wen-Hui, ZHANG Yun, ZHANG. Expression of Bm-TFF2 mutants in Escherichia coli andtheir cell migration-promoting activity. Zoological Research, 2011, 32(4): 379-385.  doi: 10.3724/SP.J.1141.2011.04379
    [11] CHEN Wen-Bo, WANG Xin, ZHOU Ya-Lu, DONG Hai-Yan, LIN Hao-Ran, LI Wen-Sheng. Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus). Zoological Research, 2011, 32(3): 285-292.  doi: 10.3724/SP.J.1141.2011.03285
    [12] Yu guo-yu, ZHANG Hong-Yun, ZHANG Yong, JIANG Ping, Lee Wen-Hui, Zhang Yun. Expression and identification of Bm-TFF2 in Pichia pastoris. Zoological Research, 2010, 31(6): 565-569.  doi: 10.3724/SP.J.1141.2010.06565
    [13] ZHAO Hui, LIU Xiao-dong, GAO Zhen-hua, ZHANG Jian-hua, LI Hong, ZHANG Yun, LEE. Expression of Human Semenogelin I-52 and Antibacterial Activity Investigation of Recombinant Peptide. Zoological Research, 2008, 29(2): 139-144.  doi: 10.3724/SP.J.1141.2008.02139
    [14] DENG Si-ping, CHEN Song-lin, LIU Ben-wei, XU Jian-yong, TIAN Yong-sheng. Molecular Cloning, Characterization and Expression Analysis of Brain P450arom in Half-smooth Tongue-sole, Cynoglossus semilaevis Gunther. Zoological Research, 2008, 29(1): 17-24.
    [15] DENG Si-ping, CHEN Song-lin, LIU Ben-wei. Molecular Cloning and Expression Analysis of FTZ-F1 in the Half-smooth Tongue-sole, Cynoglossus semilaevis. Zoological Research, 2008, 29(6): 592-598.  doi: 10.3724/SP.J.1141.2008.06592
    [16] PENG Wu, TANG Ya-nan, HU Cheng-yu. Characterization of CaPKR-like Expression in Tissues from Crucian and Grass Carp. Zoological Research, 2007, 28(5): 465-469.
    [17] LIU Xu-sheng, LIANG Bing, ZHANG Shu-yi. cDNA Cloning,Tissue Distribution and mRNA Transcription of DMRT1 Gene in the Protandrous Black Porgy Acanthopagrus schlegeli. Zoological Research, 2004, 25(2): 158-161.
    [18] LI Chang-jian, LIU Jun, SHI Yao-hua, YANG Shu-ting, GUI Jian-fang. Full Length cDNA Cloning and Tissue Expression Specificity of Ran Gene in Color Crucian Carp. Zoological Research, 2003, 24(3): 173-179.
    [19] HE Yi-xun, MAO Cai-sheng, CHANG Zheng-shan, YU Qi-fang, HU Ya-qing, ZHANG Yong-nian. Infection and Maturation of Schistosoma japonicum in Inbred and Outbred Mice. Zoological Research, 1983, 4(3): 239-244.
    [20] HE Yi-xun, YANG Hui-zhong. Histochemical Studies on The Post-Cercarial Development of Schistosoma Japonicum. Zoological Research, 1980, 1(4): 453-463.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1865) PDF downloads(1757) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return