Volume 4 Issue 4
Jul.  1983
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WANG Wan-yu, YANG Chang-jiu, XIONG Yu-liang, CHEN Xi-lan. Study on the Venom of Trimeresurus mucrosquamatus Ⅰ.Isolation of T.mucrosquamatus Venom and the Determination of its Biological and Enzymatic Activities. Zoological Research, 1983, 4(4): 327-332.
Citation: WANG Wan-yu, YANG Chang-jiu, XIONG Yu-liang, CHEN Xi-lan. Study on the Venom of Trimeresurus mucrosquamatus Ⅰ.Isolation of T.mucrosquamatus Venom and the Determination of its Biological and Enzymatic Activities. Zoological Research, 1983, 4(4): 327-332.

Study on the Venom of Trimeresurus mucrosquamatus Ⅰ.Isolation of T.mucrosquamatus Venom and the Determination of its Biological and Enzymatic Activities

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1983-11-22
  • By means of DEAE-sephadex A-50 column chromatography,Trimeresurus mucrosquamqtus venom was separated into fourteen fractions.It contained proteolytic enzyme,5-nucleotidase,arginine esterase,etc.The enzymatic activities and biological activities of crude venom and each protein fraction were determined.The proteolytic enzyme was concentrated in the fractions 1,2,4,7,and 8.Among them the fraction 1 had the highest activity of proteolytic enzyme.Fraction 2 possessed phospholipase A activity,but not the others.Fraction 7-14 possessed arginine esterase activity.Fractions 10-14 possessed hemorrhagic principles and among them fraction 12 was the highest.Fractions 1,12-14 possessed lethal toxicity.Fractions 11-13 showed the effect of platelet aggregation.
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