Volume 16 Issue 4
Jul.  1995
Turn off MathJax
Article Contents
ZHANG Ya-ping, WANG Wen, SU Bing, Oliver A.Ryder, FANG Zhi-yong, ZHANG He-min et al.. Microsatellite Dnas and Kinship Identification of Giant Panda. Zoological Research, 1995, 16(4): 301-306.
Citation: ZHANG Ya-ping, WANG Wen, SU Bing, Oliver A.Ryder, FANG Zhi-yong, ZHANG He-min et al.. Microsatellite Dnas and Kinship Identification of Giant Panda. Zoological Research, 1995, 16(4): 301-306.

Microsatellite Dnas and Kinship Identification of Giant Panda

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1995-11-22
  • We constructed a DNA library of 150-500 bp fragments from giant panda genomic DNA digested with DpnⅡ.Ten microsatellite DNA loci were isolated and characterized by screening the library with a [32]P-labelled (CA)[15] probe.The specific primers for each of the microsatellite DNA locus were synthesized and employed to amplify DNA samples isolated from 7 specimens of panda tissue and 6 specimens of hair.Nine of the 10 microsatellite DNA loci were polymorphic in the 13 samples.Inheritance patterns of alleles at these loci accurately agree with the well recorded pedigree.The polymorphism on these loci also clarified some previously unresolved paternity.These results imply the screened microsatellite DNA loci are useful makers for the identification of kinship among giant panda.
  • loading
  • Relative Articles

    [1] Hai-Tao Zhao, Wen-Qing Zhang, Kang-Sheng Jia, Jia-Xuan Li, Xu-Xiang Bai, Xiao-Wei Wang, Song-Tao Guo, Shu-Jun He, Hong-Juan Sun, Ying-Hu Lei, Ru-Liang Pan, Bao-Guo Li. Observation of mother-perpetrated infanticide in golden takins (Budorcas taxicolor bedfordi). Zoological Research, 2020, 41(4): 455-457.  doi: 10.24272/j.issn.2095-8137.2020.041
    [2] Li-Xian YIN, Lei ZHANG, Peng CHANG, Jing LI, Dong-Mei WAN. Extrapair paternity in Parus major. Zoological Research, 2013, 34(1): 47-52.  doi: 10.3724/SP.J.1141.2013.01047
    [3] WAN Yu-Ling, ZHANG Yan-Chun, PENG Bai-Lu, LI Xue-Jia, JI Fang, JIN Li-Sha, RAO Ju. Screening of spontaneous diabetes mellitus in middle- and old-aged cynomolgus monkey. Zoological Research, 2011, 32(3): 307-310.  doi: 10.3724/SP.J.1141.2011.03307
    [4] HUO Jin-long, HUO Hai-long, MIAO Yong-wang, LI Fu-quan, LIU Li-xian, WU Ge-min, OUYAN. Genetic Diversity of 76 STR Loci in the Dahe Pig. Zoological Research, 2009, 30(1): 105-108.  doi: 10.3724/SP.J.1141.2009.01105
    [5] CHANG Yu-mei, XU Wan-tu, CHI Bing-jie, GAO Guo-qiang, HAN Qi-xia, HE Wei, SUN Xiao-we. Kinship Analysis of One Broodstock Population of Large Yellow Croaker Pseudosciaena crocea. Zoological Research, 2009, 30(6): 620-626.  doi: 10.3724/SP.J.1141.2009.06620
    [6] , , , , , . Construction Fractional Genomic Libraries and Screening Microsatellites DNA of Esox reieherti Dybowski. Zoological Research, 2008, 29(3): 245-252.  doi: 10.3724/SP.J.1141.2008.03245
    [7] LIU Qing-shen, FENG Ding-yuan, ZHANG Hui-ming, LIU Shen-fu. Genetic Diversity of Chinese Shar-pei Dog Using Microsatellite DNA Markers. Zoological Research, 2008, 29(1): 31-36.
    [8] CHENG Fei, YE Wei, *, YE Fu-liang. Isolation of Microsatellite DNA and Preliminary Genomic Analysis of Mud Carp (Cirrhina molitorella) (in English). Zoological Research, 2007, 28(2): 119-125.
    [9] TIAN Hong, WEI Rong-ping, ZHANG Gui-quan, SUN Ru-yong, LIU Ding-zhen, *. Age Differences in Behavioral Responses of Male Giant Pandas to Chemosensory Stimulation (in English). Zoological Research, 2007, 28(2): 134-140.
    [10] YANG Ze-yu, MIAO Yong-wang , LI Da-lin, HUO Jin-long, CHEN Tao, HE Chao-yang, CHUANG Xiang-hui, TANG Shou-kun. Unfolding of Population Structure in Dehong Buffalo Using Microsatellite DNA Markers. Zoological Research, 2007, 28(6): 659-663.
    [11] ZHANG Ming, HOU Rong, LIU Yu-liang, ZHENG Hong-pei, ZHU Qing, ZHANG Zhi-he, XIAN Hong. Effects of Epidermal Growth Factor and Insulin on Biological Characteristics of Giant Panda (Ailuropoda melanoleuca) Cutaneous Fibroblast in Vitro. Zoological Research, 2005, 26(5): 499-505.
    [12] TIAN Hong, WEI Rong-ping, ZHANG Gui-quan, SUN Ru-yong, LIU Ding-zhen. Behavioral Difference of Sub-adult Giant Pandas (Ailuropoda melanoleuca) in Traditional and Semi-natural Enclosures. Zoological Research, 2004, 25(2): 137-140.
    [13] YIN Bao-fa, WEI Wan-hong, ZHANG Yan-min, WANG Jin-long, CAO Yi-fan. Application of Microsatellite Technology in Reproductive Behavior of Plateau Pika,Ochotona curzoniae. Zoological Research, 2003, 24(6): 401-406.
    [14] FEI Li-Song, CHEN Hong-Wei, ZHENG Yu-Cai, WANG Qiang, WANG Jie, LI Guang-Han, ZHANG An-Jiu. Protein Composition of the Giant Panda Milk. Zoological Research, 2001, 22(5): 421-424.
    [15] HU Jie, HU Jin-chu, QU Zhi-biao, YANG Dong-lei. The Study on The Giant Pands's Selection and Utilization to Fargesia nitida in Huanglong. Zoological Research, 2000, 21(1): 48-51.
    [16] HOU Wan-Ru. Amendment on The Life Table and Relevant Datum Indicators of Giant Panda. Zoological Research, 2000, 21(5): 361-366.
    [17] HU Jie, HU Jin-Chu, QU Zhi-Biao, YANG Dong-Lei. Population and Age Structure of Giant Panda in Huanglongsi Nature Reserve,Sichuan,China. Zoological Research, 2000, 21(4): 287-290.
    [18] LIAO Dang-jing, GUAN Guo-jun, WANG Xiong-qing. Electrophoretic Analysis on Three Kinds of Serum Isoenzymes From Giant Panda. Zoological Research, 1992, 13(4): 341-342.
    [19] ZHANG Ya-ping, CHEN Xing, SHI Li-ming. Restriction Enzyme Map of mtDNA From Giant Panda (Ailuropoda melanoleuca). Zoological Research, 1991, 12(2): 209-214.
    [20] ZHANG Da-zhong CHEN Su-qing CHEN Jian-xuan et al.HE Guang-xin FENG Wen-he. Relations Between Disease and Trace Elements in Giant Panda Hair. Zoological Research, 1991, 12(1): 73-78.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2613) PDF downloads(1825) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint