Volume 13 Issue 4
Jul.  1992
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ZHANG Dong-lei, WANG Qi-zheng, CHENG Jian-zhi. Enzymatic Properties and Biological Activities of A Proteolytic Enzyme (Proteinase A) From The Venom of Agkistrodon Halys Pallas. Zoological Research, 1992, 13(4): 381-385.
Citation: ZHANG Dong-lei, WANG Qi-zheng, CHENG Jian-zhi. Enzymatic Properties and Biological Activities of A Proteolytic Enzyme (Proteinase A) From The Venom of Agkistrodon Halys Pallas. Zoological Research, 1992, 13(4): 381-385.

Enzymatic Properties and Biological Activities of A Proteolytic Enzyme (Proteinase A) From The Venom of Agkistrodon Halys Pallas

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1992-11-22
  • A proteolytic enzyme from the venom of Agkistrodon halys Pallas has been purified by ion-exchang chromatography and by gel-filtration.This proteolytic enzyme (called proteinase a) can hydrolyze casein.Using casein as the substrate,the optimum pH is about 9.5,the optimum temperature is about 45℃,and Km=1.33×10[-5] mol/L.Its activity toward casein was inhibited by EDTA.It did not act on TAME and BAEE.Proteinase a showed fibrinolytic activity in vitro,EDTA and cysteine could inhibite this activity.
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