Volume 12 Issue 1
Jan.  1991
Turn off MathJax
Article Contents
ZHENG Zi-xiu, ZHONG Jin-yan. Studies on The Lactate Dehydrogenase Isoenzymes of Tree Shrew (Tupaia belangeri chinensis) Tissues:An Electrophoretic Analysis on The Agarose Gel ). Zoological Research, 1991, 12(1): 85-91.
Citation: ZHENG Zi-xiu, ZHONG Jin-yan. Studies on The Lactate Dehydrogenase Isoenzymes of Tree Shrew (Tupaia belangeri chinensis) Tissues:An Electrophoretic Analysis on The Agarose Gel ). Zoological Research, 1991, 12(1): 85-91.

Studies on The Lactate Dehydrogenase Isoenzymes of Tree Shrew (Tupaia belangeri chinensis) Tissues:An Electrophoretic Analysis on The Agarose Gel )

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 1991-02-22
  • Lactate dehydrogenase (EC 1.1.1.27) is an oxidoreductase which catalyzes the resversion conversion of phruvate to lactate.The five isoenzymes of lactate dehydrogenase are tetramers composed of four polypeptide chains of two types subunits A and B (or M and H).The five isoenzymes have been demonstrated in all mammalian tissues,cells and serum.Tree shrew is a specific kind of animal between insectivora and primate.It is one of the most suitable research materials for experimental medicine,experimental pathology and evolutional biology.The present paper deals with the analysis by agarose gel electrophoresis of lactate dehydrogenase isoenzymes of eight different tissues of tree shrew (Tupaia belangeri chinensis).The eight tissues are:heart,lung,submaxillary gland,spleen,kidney,adrenal gland,skeletal muscle and brain.The electrophoresis was performed on 0.5% agarose gel thin layer in a Ph 8.6 buffer containing 0.06 mol/L sodium diethylbarbiturate,0.2 N hydrochloric acid.The isoenzymic activity was visualized with a stanining solution consisting of sodium DL-lactate,nicotinamide adenine dinucleotide (NAD),N-methylphenazinium methylsulphate (PMS) and nitro-tetrazolium blue chloride (NBT).The electropherograms of LDH isoenzymes was scanned record at λs=570 nm and λr=500 nm,scanning speed for 20 mm/min.Five different molecular forms of isoenzymes and alterations of isoenzymic patterns were obserbed in the eight tissues of tree shrew.The isoenzymic phenotypes of five molecular forms are LCH1,LDH2,LDH3,LDH4,LDH5,in heart,submaxillary gland and brain,only the phenotype of skeletal muscle were LDH5,LDH4,LDH3,LDH2,LDH1.The LDH4 to LDH1 isoenzymes migrated towards the anode and LDH5 isoenzyme toward the cathode in the Ph 8.6 buffer.LDH1 is the fastest and LDH5 the slowest in the electrophoresis.The mean values of the five components of LDH isoenzymes in eight itssues were measured.The percentage of H subunit was calculated with the formula H%=LDH1%+3/4 LDH2%+1/2 LDH3%+1/4 LDH4%.The H/M subunits ratio was 2.3,0.9,1.5,1.0,1.6,0.94,0.55 and 1.8 in above mentioned eight tissues,respectively.
  • loading
  • Relative Articles

    [1] Shu Wei, Hai-Rong Hua, Qian-Quan Chen, Ying Zhang, Fei Chen, Shu-Qing Li, Fan Li, Jia-Li Li. Dynamic changes in DNA demethylation in the tree shrew (Tupaia belangeri chinensis) brain during postnatal development and aging. Zoological Research, 2017, 38(2): 96-102.  doi: 10.24272/j.issn.2095-8137.2017.013
    [2] Jian-Ping LI, Yun LIAO, Ying ZHANG, Jing-Jing WANG, Li-Chun WANG, Kai FENG, Qi-Han LI, Long-Ding LIU. Experimental infection of tree shrews(Tupaia belangeri) with Coxsackie virus A16. Zoological Research, 2014, 35(6): 485-491.  doi: 10.13918/j.issn.2095-8137.2014.6.485
    [3] Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews. Zoological Research, 2014, 35(6): 492-499.  doi: 10.13918/j.issn.2095-8137.2014.6.492
    [4] Ling XU, Yu FAN, Xue-Long JIANG, Yong-Gang YAO. Molecular evidence on the phylogenetic position of tree shrews. Zoological Research, 2013, 34(2): 70-76.  doi: 10.3724/SP.J.1141.2013.02070
    [5] Xiao-Yun WU, Yun-Hai LI, Qing CHANG, Lin-Qiang ZHANG, Sha-Sha LIAO, Bin LIANG. Streptozotocin induction of type 2 diabetes in tree shrew. Zoological Research, 2013, 34(2): 108-115.  doi: 10.3724/SP.J.1141.2013.02108
    [6] ZHANG Yuan-Xu, PING Shu-Huang, YANG Shi-Hua. Morphological characteristics and cryodamage of Chinese tree shrew (Tupaia belangeri chinensis) sperm. Zoological Research, 2012, 33(1): 29-36.  doi: 10.3724/SP.J.1141.2012.01029
    [7] LI Sheng-An, LEE Wen-Hui, ZHANG Yun. Two bacterial infection models in tree shrew for evaluating the efficacy of antimicrobial agents. Zoological Research, 2012, 33(1): 1-6.  doi: 10.3724/SP.J.1141.2012.01001
    [8] SUN Yong-Mei, YANG Jian-Zhen, SUN Hua-Ying, MA Yuan-Ye, WANG Jian-Hong. Establishment of tree shrew chronic morphine dependent model. Zoological Research, 2012, 33(1): 14-18.  doi: 10.3724/SP.J.1141.2012.01014
    [9] WANG Jing, ZHOU Qi-Xin, TIAN Men, YANG Yue-Xiong, XU Lin. Tree shrew models: A chronic social defeat model of depression and a one-trial captive conditioning model of learning and memory. Zoological Research, 2011, 32(1): 24-30.  doi: 10.3724/SP.J.1141.2011.01024
    [10] MA Xu-Tong, LI Fu-Lin, JIANG Hong-Jun, LI Wen-Hui, ZHANG Yun, DU Ting-Yi. Detection and comparison of physiological indexes in the wild and laboratory tree shrew. Zoological Research, 2011, 32(1): 4-10.  doi: 10.3724/SP.J.1141.2011.01004
    [11] ZHANG Ding, GAO Li, ZHANG Yuan-xu, SUN Li, FENG Yue, HE You-wen, XIA Xue-shan, ZHAN. Crucial Factors for de novo Establishment of Long-term Primary Culture of Tree Shrew Hepatocytes. Zoological Research, 2009, 30(1): 24-30.  doi: 10.3724/SP.J.1141.2009.01024
    [12] YANG Min, ZHANG Chi-yu, BEN Kun-long. Cloning and Sequence Analysis of Cyclin T1 cDNA from Tree Shrew (Tupaia belangeri). Zoological Research, 2003, 24(3): 205-210.
    [13] ZHANG Li, BEN Kun-Long. In vitro Infection of Tree Shrew Immunocytes with Human Immunodeficiency Virus Type Ⅰ. Zoological Research, 2001, 22(1): 33-40.
    [14] CAO Xiao-mei. Microscopic Structure of Ovary and Ovarian Activity of Different Seasons in Tree Shrew (Tupaia belangeri chinensis). Zoological Research, 1990, 11(1): 17-24.
    [15] YE Zhi-zhang, PENG Yen-zhang, PAN Ru-liang, WANG Hong. Arterial System in Chinese Tree Shrew (Tupaia belangeri chinensis). Zoological Research, 1990, 11(2): 131-138.
    [16] ZOU Ru-jin, TIAN Bao-pin, JI Wei-zhi, LI Rui-yuan. Research on The Piebaldism of Tree Shrews Breeding in The Captivity. Zoological Research, 1989, 10(zk): 68-78.
    [17] CAO Xiao-mei. Seasonal Changes in Spermatogenesis of Tree Shrew (Tupaia Belangeri Chinensis). Zoological Research, 1989, 10(1): 15-21.
    [18] MA Cai-xia, MA Kun, SHI Li-ming. Electron Microscopic Observations on The Synaptonemal Complex in Spermatocytes of The Tree Shrew (Tupaiabelangeri Chinensis). Zoological Research, 1989, 10(zk): 29-33.
    [19] ZHANG Ya-ping, ZHANG Bing, SHI Li-ming. Restriction Maps of Mitochondrial DNA of Slow Loris and Tree Shrew. Zoological Research, 1989, 10(zk): 79-89.
    [20] ZHENG Zi-xiu, ZHONG Jin-yan, DING Lin-hua. Studies on Lactate Dehydrogenase Isoenzymes of the Marine Dinoflagellate (Oxyrrhis marina):An Analysis by Agarose gel Electrophoresis. Zoological Research, 1988, 9(zk): 33-42.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2238) PDF downloads(1367) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return