Volume 25 Issue 5
Sep.  2004
Turn off MathJax
Article Contents
SHI Yan, WU Xiao-bing, YAN Peng, CHEN Bi-hui. Cloning and Sequences Analysis of the Second Exon of MHC Class Ⅱ B Genes in Chinese Alligator,Alligator sinensis. Zoological Research, 2004, 25(5): 415-421.
Citation: SHI Yan, WU Xiao-bing, YAN Peng, CHEN Bi-hui. Cloning and Sequences Analysis of the Second Exon of MHC Class Ⅱ B Genes in Chinese Alligator,Alligator sinensis. Zoological Research, 2004, 25(5): 415-421.

Cloning and Sequences Analysis of the Second Exon of MHC Class Ⅱ B Genes in Chinese Alligator,Alligator sinensis

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2004-10-22
  • A pair of degenerate primers were used to amplify polymorphic segments of the second exon of MHC class Ⅱ B genes from the genomic DNA of Alligator sinensis.Sequences were obtained from cloning,single strand conformation polymorphism analysis and sequencing.By comparing with another eight species MHC sequences,differences from sequences and variable sites were confirmed.Phylogenetic relationships were constructed by neighbor-joining method in MEGA and maximum parsimony method in PAUP4.0.Ten different nucleotide sequences were obtained,which were 166 bp long (except one 160 bp).There are 38 variable sites among ten nucleotide sequences and 23 variable sites among amino acid sequences.The number of nonsynonymous (dN) substitutions of pABS was higher than that of synonymous (dS) substitutions.The ten nucleotide sequences can be clustered into two clades (group A and B) in NJ and MP phylogenetic trees.Some specific sites were found between the two groups,including 9 nucleotide sites and 7 amino acid sites.The high polymorphism of MHC class Ⅱ B genes in A.sinensis was of great benefit to genetic conservation in the captive population of A.sinensis.
  • loading
  • Relative Articles

    [1] Xi-Yao Ma, Tiao Ning, Adeniyi C. Adeola, Jie Li, Ali Esmailizadeh, Jacqueline K. Lichoti, Bernard R. Agwanda, Jainagul Isakova, Almaz A. Aldashev, Shi-Fang Wu, He-Qun Liu, Najmudinov Tojiddin Abdulloevich, Manilova Elena Afanasevna, Khudoidodov Behruz Ibrohimovich, Rahamon Akinyele Moshood Adedokun, Sunday Charles Olaogun, Oscar J. Sanke, Godwin F. Mangbon, Xi Chen, Wei-Kang Yang, Zhe Wang, Min-Sheng Peng, Sheila C. Ommeh, Yan Li, Ya-Ping Zhang. Potential dual expansion of domesticated donkeys revealed by worldwide analysis on mitochondrial sequences. Zoological Research, 2020, 41(1): 51-60.  doi: 10.24272/j.issn.2095-8137.2020.007
    [2] Si-Qing LIU, Qiong-Ying TANG, Xiao-Juan LI, Huan-Zhang LIU. Phylogeographic analyses of Sarcocheilichthys nigripinnis(Teleostei:Cyprinidae)based on mitochondrial DNA Cyt b gene sequences. Zoological Research, 2013, 34(5): 437-445.  doi: 10.11813/j.issn.0254-5853.2013.5.0437
    [3] WU Yun-Mei, ZHANG Li-Hong, WANG Guo-Xiu. Analysis of differentially expressed genes from female and male postparasitic Ovomermis sinensis juveniles. Zoological Research, 2012, 33(5): 487-492.  doi: 10.3724/SP.J.1141.2012.05487
    [4] CHEN Xiao-wu, SHI Zhi-yi, CHENG Qian-qian. Full-length cDNA Cloning and Tissues Expression Analysis of Ferritin Gene from Acipenser sinensis. Zoological Research, 2009, 30(2): 144-150.  doi: 10.3724/SP.J.1141.2009.02144
    [5] LIANG Gang, LI Tao, YIN Zuo-hua, LEI Fu-min. Molecular Phylogenetic Analysis of Some Fringillidae Species Based on Mitochondrial CoI Gene Sequences. Zoological Research, 2008, 29(5): 465-475.  doi: 10.3724/SP.J.1141.200805465
    [6] WU Dong-xia, HAO Jia-sheng * , ZHU Guo-ping, CHEN Na, SU Cheng-yong, PAN Hong-chun, ZHANG Xiao-ping. Phylogenetic Relationships of Butterflies in the Subfamily Limenitinae Based on Mitochondrial Cytochrome b Gene Sequences. Zoological Research, 2007, 28(1): 1-8.
    [7] ZHANG Fang, WU Xiao-bing , *, MENG Wei-zhi, ZHU Jia-long. Ecology on the Making Nest and Laying Eggs of Chinese Alligator (Alligator sinensis) under Artificial Feeding Conditions. Zoological Research, 2006, 27(2): 151-156.
    [8] WANG Qian, WU Xiao-bing* , YAN Peng. Sequence Variability Analysis of MHC Class Ⅱ DRB Alleles in Three Felines. Zoological Research, 2006, 27(2): 181-188.
    [9] DAI Jin-xia, ZHENG Zhe-min. Discussion on the Phylogenetic Relationships of Partial Pentatomidae Insects Based on Sequences of Cytochrome b Gene. Zoological Research, 2004, 25(5): 397-402.
    [10] FU Cheng-jie, MIAO Wei, SHEN Yun-fen, WAN Ming-liang. Phylogenetic Analysis of Peritrichs Inferred from Cytosolic Hsp70 Gene Partial Sequences. Zoological Research, 2004, 25(4): 298-303.
    [11] CHEN Jing-hua, Paul CROW, Etsuo NARUSHIMA, ZHANG Hong-wei, ZHANG Ya-ping. Molecular Phylogeny of Slow Lorises (Nycticebus) Revealed by D-loop Sequences and Complete Cytochrome b Gene Sequences of Mitochondrial DNA. Zoological Research, 2004, 25(4): 292-297.
    [12] LI Wei, ZHANG Yan-yun. Subspecific Taxonomy of Ficedula parva Based on Sequences of Mitochondrial Cytochrome b Gene. Zoological Research, 2004, 25(2): 127-131.
    [13] HUA Tian-miao, WANG Chao-lin, CHEN Bi-hui. Stages of Embryonic Development for Alligator sinensis. Zoological Research, 2004, 25(3): 263-271.
    [14] WU Jian-sheng, WANG Xiao-ming. Regulation of Bellowing of Chinese Alligators (Alligator sinensis) in the Wild. Zoological Research, 2004, 25(4): 281-286.
    [15] ZHAN Xiang-jiang, ZHANG Zheng-wang, WU Ai-ping, Tao Yu-jing. Phylogenetic Relationships of Monal Pheasants Lophophorus Inferred from Sequences of Mitochondrial Cytochrome b Gene. Zoological Research, 2003, 24(5): 337-342.
    [16] CHEN Dong-sheng, NIE Liu-wang, CHENG Shuang-huai, KAN Xian-zhao, WANG Chao-lin, XIE Wan-shu. Amplification and Sequencing of Sox Gene HMG-box in Alligator sinensis. Zoological Research, 2003, 24(2): 127-131.
    [17] SHAO Zhi-yong-, MAO Hong-xin, FU Wen-jun, ZHANG Ya-ping. Phylogenetic Relationships of 11 Bumblebee Species (Hymenoptera:Apidae) Based on Mitochondrial Cytochrome b Gene Sequences. Zoological Research, 2002, 23(5): 361-366.
    [18] DING You-Zhong, WANG Xiao-Ming, HE Li-Jun, WANG Ren-Ping, XIE Wan-Shu. A Primelinary Study on Heavy Metal Elements (Cu,Zn,Cd and Pb) in Different Parts of the Eggs of Chinese Alligator (Alligator sinensis). Zoological Research, 2001, 22(3): 253-256.
    [19] HE Shun-ping, CHEN Yong-jiu, ZHANG Ya-ping. Preliminary Study on Mitochondrial Cytochrome b DNA Sequences and Phylogeny of Formalin Fixed Sisorid Fishes. Zoological Research, 1999, 20(2): 81-87.
    [20] RUAN Rong-wen, SONG Ru-gai, ZHANG Zheng-dong. A Tentative Report on the Elecrocardiogram (ECG) Study of Chinese Alligator. Zoological Research, 1988, 9(zk): 0-90.
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1793) PDF downloads(2037) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint