Volume 40 Issue 3
May  2019
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Cheng Xiang, Zhi-Na Li, Tian-Zhuang Huang, Jing-Hui Li, Lei Yang, Jing-Kuan Wei. Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice[J]. Zoological Research, 2019, 40(3): 231-235. doi: 10.24272/j.issn.2095-8137.2019.038
Citation: Cheng Xiang, Zhi-Na Li, Tian-Zhuang Huang, Jing-Hui Li, Lei Yang, Jing-Kuan Wei. Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice[J]. Zoological Research, 2019, 40(3): 231-235. doi: 10.24272/j.issn.2095-8137.2019.038

Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice

doi: 10.24272/j.issn.2095-8137.2019.038
Funds:  This study was supported by the National Natural Science Foundation of China (81403191) and Yunnan Provincial Natural Science Foundation (2018FB118, 2015FA004, and KKSY201626001)
More Information
  • Corresponding author: Jing-Kuan Wei
  • Received Date: 2018-08-30
  • Publish Date: 2019-05-18
  • Early brain development after birth is extremely dynamic, suggesting that potential functional changes occur during this period. In this study, the maximal electroshock seizure threshold (MEST) was used to explore the electrophysiological variation among three developmental stages in young mice (no more than 5 weeks old). The induced electroshock seizure (ES) behavior of early postnatal mice (1–2-weeks old) differed from that during weaning (3 weeks old) and early puberty (4–5-weeks old). Thus, we further explored their respective characteristic responses to the ES parameters. When the stimulation current (SC) was limited to 4.0 mA, only the 1–2-week-old mice were induced to exhibit ES behavior at voltages of 30 V and 40 V, indicating they were more sensitive to maximal electroshock seizure (MES) (response to lower voltage). Surprisingly, however, they showed substantially lower mortality than the older groups under higher voltage conditions (60, 100, 160, and 200 V), suggesting better tolerance to the SC. We also found that when the current limit decreased to 3.5 mA, the 4–5-week-olds mice exhibited stable ES behavior with low mortality, while for 3-week-olds mice, the SC limit required to be reduced to 1.5 mA. In conclusion, our findings showed that neural sensitivity to MES was significantly different in young mice before puberty. Thus, greater attention should be given to distinguishing the developmental period of mice, especially in electrophysiological examination.
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Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice

doi: 10.24272/j.issn.2095-8137.2019.038
Funds:  This study was supported by the National Natural Science Foundation of China (81403191) and Yunnan Provincial Natural Science Foundation (2018FB118, 2015FA004, and KKSY201626001)
    Corresponding author: Jing-Kuan Wei

Abstract: Early brain development after birth is extremely dynamic, suggesting that potential functional changes occur during this period. In this study, the maximal electroshock seizure threshold (MEST) was used to explore the electrophysiological variation among three developmental stages in young mice (no more than 5 weeks old). The induced electroshock seizure (ES) behavior of early postnatal mice (1–2-weeks old) differed from that during weaning (3 weeks old) and early puberty (4–5-weeks old). Thus, we further explored their respective characteristic responses to the ES parameters. When the stimulation current (SC) was limited to 4.0 mA, only the 1–2-week-old mice were induced to exhibit ES behavior at voltages of 30 V and 40 V, indicating they were more sensitive to maximal electroshock seizure (MES) (response to lower voltage). Surprisingly, however, they showed substantially lower mortality than the older groups under higher voltage conditions (60, 100, 160, and 200 V), suggesting better tolerance to the SC. We also found that when the current limit decreased to 3.5 mA, the 4–5-week-olds mice exhibited stable ES behavior with low mortality, while for 3-week-olds mice, the SC limit required to be reduced to 1.5 mA. In conclusion, our findings showed that neural sensitivity to MES was significantly different in young mice before puberty. Thus, greater attention should be given to distinguishing the developmental period of mice, especially in electrophysiological examination.

Cheng Xiang, Zhi-Na Li, Tian-Zhuang Huang, Jing-Hui Li, Lei Yang, Jing-Kuan Wei. Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice[J]. Zoological Research, 2019, 40(3): 231-235. doi: 10.24272/j.issn.2095-8137.2019.038
Citation: Cheng Xiang, Zhi-Na Li, Tian-Zhuang Huang, Jing-Hui Li, Lei Yang, Jing-Kuan Wei. Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice[J]. Zoological Research, 2019, 40(3): 231-235. doi: 10.24272/j.issn.2095-8137.2019.038
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