Volume 40 Issue 4
Jul.  2019
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Nikolay A. Poyarkov Jr., Vladislav A. Gorin, Than Zaw, Valentina D. Kretova, Svetlana S. Gogoleva, Parinya Pawangkhanant, Jing Che. On the road to Mandalay: contribution to the Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) fauna of Myanmar with description of two new species. Zoological Research, 2019, 40(4): 244-276. doi: 10.24272/j.issn.2095-8137.2019.044
Citation: Nikolay A. Poyarkov Jr., Vladislav A. Gorin, Than Zaw, Valentina D. Kretova, Svetlana S. Gogoleva, Parinya Pawangkhanant, Jing Che. On the road to Mandalay: contribution to the Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) fauna of Myanmar with description of two new species. Zoological Research, 2019, 40(4): 244-276. doi: 10.24272/j.issn.2095-8137.2019.044

On the road to Mandalay: contribution to the Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) fauna of Myanmar with description of two new species

doi: 10.24272/j.issn.2095-8137.2019.044
Funds:  This study was partially supported by the Russian Science Foundation (19-14-00050) to N.A.P., Southeast Asia Biodiversity Research Institute, CAS (Y4ZK111B01: 2017CASSEAB RIQG002), and Animal Branch of the Germplasm Bank of Wild Species, CAS(Large Research Infrastructure Funding)
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  • Corresponding author: Nikolay A. Poyarkov Jr.
  • Received Date: 2018-11-29
  • Publish Date: 2019-07-18
  • We present a morphological and molecular assessment of the Microhyla fauna of Myanmar based on new collections from central (Magway Division) and northern (Kachin State) parts of the country. In total, six species of Microhyla are documented, including M. berdmorei, M. heymonsi, M. butleri, M. mukhlesuri and two new species described from the semi-arid savanna-like plains of the middle part of the Irrawaddy (Ayeyarwady) River Valley. We used a 2 481 bp long 12S rRNA–16S rRNA fragment of mtDNA to hypothesize genealogical relationships within Microhyla. We applied an integrative taxonomic approach combining molecular, morphological, and acoustic lines of evidence to evaluate the taxonomic status of Myanmar Microhyla. We demonstrated that the newly discovered populations of Microhyla sp. from the Magway Division represent two yet undescribed species. These two new sympatric species are assigned to the M. achatina species group, with both adapted to the seasonally dry environments of the Irrawaddy Valley. Microhyla fodiens sp.nov. is a stout-bodied species with a remarkably enlarged shovel-like outer metatarsal tubercle used for burrowing and is highly divergent from other known congeners (P-distance≥8.8%). Microhyla irrawaddy sp. nov. is a small-bodied slender frog reconstructed as a sister species to M. kodial from southern India (P-distance=5.3%); however, it clearly differs from the latter both in external morphology and advertisement call parameters. Microhyla mukhlesuri is reported from Myanmar for the first time. We further discuss the morphological diagnostics and biogeography of Microhyla species recorded in Myanmar.
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  • [1]
    null Agarwal I , Bauer AM , Jackman TR , Karanth KP . 2014. Cryptic species and miocene diversification of palaearctic naked-toed geckos (Squamata: Gekkonidae) in the Indian dry zone. Zoologica Scripta, 43(5): 455–471.
    null Agarwal I , Karanth KP . 2015. A phylogeny of the only ground-dwelling radiation of Cyrtodactylus (Squamata, Gekkonidae): diversification of Geckoella across peninsular India and Sri Lanka. Molecular Phylogenetics and Evolution, 82: 193–199.
    null Agarwal I , Ramakrishnan U . 2017. On the antiquity of Indian grassy biomes: a phylogeny of open-habitat lizards (Squamata: Lacertidae: Ophisops) supports the antiquity of Indian grassy biomes. Journal of Biogeography, 44(9): 2021–2032.
    null Andersson LG . 1942. A small collection of frogs from Annam collected in the years 1938-39 by Bertil Bjorkegren. Arkiv for Zoologi, 34A(6): 1–11.
    null Atmaja VY , Hamidy A , Arisuryanti T , Matsui M , Smith EN . 2019. A new species of Microhyla (Anura: Microhylidae) from Sumatra, Indonesia. Treubia, 45: 25–46.
    null Bain RH , Nguyen QT . 2004. Three new species of narrow-mouth frogs (Genus: Microhyla) from Indochina, with comments on Microhyla annamensis and Microhyla palmipes . Copeia, 2004(3): 507–524.
    null Blyth E . 1856. Report for October meeting, 1855. Journal of the Asiatic Society of Bengal, 24: 711–723.
    null Boulenger GA . 1884. Descriptions of new species of reptiles and batrachians in the British Museum. Part. II. Journal of Natural History, 13(77): 396–398.
    null Boulenger GA . 1897. Descriptions of new Malay frogs. Journal of Natural History, 19(109): 106–108.
    null Boulenger GA . 1900. Descriptions of new batrachians and reptiles from the Larut Hills, Perak. Annals and Magazine of Natural History, Series 7, 6(32): 186–193.
    null Bourret R . 1942. Les Batraciens de l’Indochine. Ha Noi: Institut Océanographique de l’Indochine. (in French)
    null Das I , Haas A . 2010. New species of Microhyla from Sarawak: Old World’s smallest frogs crawl out of miniature pitcher plants on Borneo (Amphibia: Anura: Microhylidae). Zootaxa, 2571(1): 37–52.
    null Das I , Yaakob NS , Sukumaran J . 2007. A new species of Microhyla (Anura: Microhylidae) from the Malay Peninsula. Hamadryad, 31(2): 304–314.
    null Deepak V , Karanth P . 2018. Aridification driven diversification of fan-throated lizards from the Indian subcontinent. Molecular Phylogenetics and Evolution, 120: 53–62.
    null Dehling JM . 2010. Advertisement calls of two species of Microhyla (Anura: Microhylidae) from Borneo. Salamandra, 46(2): 114–116.
    null Dever JA , Fuiten AM , ? Konu , Wilkinson JA . 2012. Cryptic torrent frogs of Myanmar: An examination of the Amolops marmoratus species complex with the resurrection of Amolops afghanus and the identification of a new species. Copeia, 2012(1): 57–76.
    null Dever JA . 2017. A new cryptic species of the Theloderma asperum complex (Anura: Rhacophoridae) from Myanmar. Journal of Herpetology, 51(3): 425–436.
    null Duméril AHC , Bibron G . 1841. Erpétologie Genérale ou Histoire Naturelle Complète des Reptiles. Vol. 8. Paris: Librarie Enclyclopedique de Roret, III– 792. (in French)
    null Dutta SK . 1997. Amphibians of India and Sri Lanka. Bhubaneswar: Odyssey Publishing House, 342.
    null Dutta SK , Ray P . 2000. Microhyla sholigari, a new species of microhylid frog (Anura: Microhylidae) from Karnataka, India. Hamadryad, 25: 38–44.
    null Fei L , Ye C , Jiang J . 2010. Colored Atlas of Chinese Amphibians. Sichuan: Sichuan Publishing Group, Sichuan Publishing House of Science & Technology, 519.
    null Felsenstein J . 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4): 783–791.
    null Fernando P , Siriwardhane M . 1996. Microhyla karunaratnei (Anura: Microhylidae), a new species of frog endemic to Sri Lanka. Journal of South Asian Natural History, 2(1): 135–142.
    null Frost DR . 2019 Amphibian Species of the World: an Online Reference. Version 6.0 (22/01/2019). New York, USA: American Museum of Natural History, Electronic database accessible at http: //research.amnh.org/herpetology/amphibia/index.html.
    null Garcia-Rutledge EJ , Narins PM . 2001. Shared acoustic resources in an old-world frog community. Herpetologica, 57(1): 104–116.
    null Garg S , Das A , Kamei RG , Biju SD . 2018a. Delineating Microhyla ornata (Anura, Microhylidae): mitochondrial DNA barcodes resolve century-old taxonomic misidentification. Mitochondrial DNA Part B, 3(2): 856–561.
    null Garg S , Suyesh R , Das A , Jiang JP , Wijayathilaka N , Amarasinghe AAT , Alhadi F , Vineeth KK , Aravind NA, Senevirathne G , Meegaskumbura M , Biju SD . 2018b. Systematic revision of Microhyla (Microhylidae) frogs of South Asia: a molecular, morphological, and acoustic assessment. Vertebrate Zoology, 69(1): 1–71.
    null Glaw F , Vences M . 1997. Anuran eye colouration: definitions, variation, taxonomic implications and possible functions. Herpetologia Bonnensis. SEH Proceedings, Bonn, 125–138.
    null Grismer LL , PL Jr Wood , Quah ESH , Murdoch ML , Grismer MS , Herr MW , Espinoza RE , Brown RM , Lin A . 2018a. A phylogenetic taxonomy of the Cyrtodactylus peguensis group (Reptilia: Squamata: Gekkonidae) with descriptions of two new species from Myanmar. PeerJ, 6: e5575; doi: 10.7717/peerj.5575
    null Grismer LL , Wood, Jr PL , Quah ESH , Thura MK , Espinoza RE , Grismer MS , Murdoch ML , Lin A . 2018b. A new species of crocodile newt Tylototriton (Caudata: Salamandridae) from Shan State, Myanmar (Burma). Zootaxa, 4500(4): 442–573.
    null Grismer LL , PL Jr Wood , Thura MK , Grismer MS , Brown RM , Stuart BL . 2018c. Geographically structured genetic variation in Ptychozoon lionotum (Squamata: Gekkonidae) and a new species from an isolated volcano in Myanmar. Zootaxa, 4514(2): 202–214.
    null Grismer LL , PL Jr. Wood , Thura MK , Zin T , Quah ESH , Murdoch ML , Grismer MS , Lin A , Kyaw H , Ngwe L . 2017a. Twelve new species of Cyrtodactylus Gray (Squamata: Gekkonidae) from isolated limestone habitats in east-central and southern Myanmar demonstrate high localized diversity and unprecedented microendemism. Zoological Journal of the Linnean Society, 182(4): 862–959.
    null Grismer LL , PL Jr. Wood , Thura MK , Zin T , Quah ESH , Murdoch ML , Grismer MS , Lin A , Kyaw H , Ngwe L . 2017b. Phylogenetic taxonomy of Hemiphyllodactylus Bleeker, 1860 (Squamata: Gekkonidae) with descriptions of three new species from Myanmar. Journal of Natural History, 52(13–16): 881–915.
    null Hall TA . 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic Acids Symposium Series.London: Information Retrieval Ltd., c1979 -c 2000, 95–98.
    null Hallermann J , Ananjeva N , Orlov N , Tillack F . 2002. Leonardo Fea’s historical collections of Amphibia and Reptilia from Burma deposited at the Zoologisches Museum Hamburg. Mitteilungen aus dem Hamburgischen Zoologischen Museum und Institut, 99: 139–153.
    null Hallowell E . 1861. Report upon the Reptilia of the North Pacific Exploring Expedition, under command of Capt. John Rogers, U.S.N. Proceedings of the Academy of Natural Sciences of Philadelphia, 1860: 480–510.
    null Hasan M , Islam MM , Khan MMR , Alam MS , Kurabayashi A , Igawa T , Kuramoto M , Sumida M . 2012. Cryptic anuran biodiversity in Bangladesh revealed by mitochondrial 16S rRNA gene sequences. Zoological Science, 29(3): 162–172.
    null Hasan M , Islam MM , Kuramoto M , Kurabayashi A Sumida M . 2014. Description of two new species of Microhyla (Anura: Microhylidae) from Bangladesh. Zootaxa, 3755(5): 401–408.
    null Hasan M , Razzaque MA , Sarker AK , Kuramoto M Sumida M . 2015. Genetic variation, advertisement call, and morphometry of Microhyla nilphamariensis from Bangladesh. Philippine Journal of Systematic Biology, 9: 63–80.
    null Hedges SB . 1994. Molecular evidence for the origin of birds. Proceedings of the National Academy of Sciences, 91(7): 2621–2624.
    null Heyer WR . 1971. Mating calls of some frogs from Thailand. Fieldiana Zoology, 58(6): 61–82.
    null Hillis DM , Moritz C , Mable BK , Graur D . 1996. Molecular Systematics (Vol. 23). Sunderland, MA: Sinauer Associates.
    null Howlader MSA , Nair A , Gopalan SV , Meril? J . 2015. A new species of Microhyla (Anura: Microhylidae) from Nilphamari, Bangladesh. PLoS One, 10(3): e0119825.
    null Hu SC . 1966. A herpetological survey of the Tsinling and Ta-Pa Shan region. Acta Zoologica Sinica, 18: 57–89. (in Chinese).
    null Huelsenbeck JP , Hillis DM . 1993. Success of phylogenetic methods in the four-taxon case. Systematic Biology, 42(3): 247–264.
    null Inger RF , Frogner KJ . 1979. New species of narrow-mouth frogs (genus Microhyla) from Borneo. The Sarawak Museum Journal, 27: 311–322.
    null Inger RF . 1989. Four new species of frogs from Borneo. Malayan Nature Journal. Kuala Lumpur, 42: 229–243.
    null IUCN Standards and Petitions Subcommittee . 2017[12 November 2018]. Guidelines for Using the IUCN Red List Categories and Criteria. Version 13. Prepared by the Standards and Petitions Subcommittee. Downloadable from: http: // .
    null Jerdon TC . 1854. Catalogue of reptiles inhabiting the Peninsula of India. Journal of the Asiatic Society of Bengal, 22: 522–534.
    null Kanamadi RD , Hiremath CR , Schneider H . 1994. Courtship, amplexus and advertisement call of the frog, Microhyla rubra . Current Science, 66: 683–684.
    null Katoh K , Misawa K , Kuma K , Miyata T . 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research, 30(14): 3059–3066.
    null Khatiwada JR , Shu GC , Wang SH , Thapa A , Wang B , Jiang J . 2017a. A new species of the genus Microhyla (Anura: Microhylidae) from Eastern Nepal. Zootaxa, 4254(2): 221–239.
    null Khatiwada JR , Wang S , Shu G , Xie F , Jiang J . 2017b. The mitochondrial genome of the Microhyla taraiensis (Anura: Microhylidae) and related phylogenetic analyses. Conservation Genetics Resources, 10(3): 441–444.
    null Kozlov A , Darriba D , Flouri T , Morel B , Stamatakis A . 2018. RAxML-NG: A fast, scalable, and user-friendly tool for maximum likelihood phylogenetic inference. BioRxiv, 447110.
    null Kuramoto M , Joshy SH . 2006. Morphological and acoustic comparisons of Microhyla ornata, M. fissipes, and M. okinavensis (Anura: Microhylidae). Current Herpetology, 25(1): 15–27.
    null Kurniati H . 2013. Vocalizations of Microhyla achatina Tschudi, 1838 (Anura: Microhylidae) from the foot hills of Mount Salak, West Java. Jurnal Biologi Indonesia, 9(2): 301–310.
    null Le DT , Ninh TH , Luong MA , Nguyen TQ . 2016a. Advertisement call and description of the tadpole of Microhyla marmorata Bain & Nguyen, 2004 from Xuan Son National Park, Phu Tho Province. Vietnam Journal of Biology, 38(2): 154–164.
    null Le DT , Ninh TH , Luong MA , Nguyen LHS , Tran DL . 2016b. Advertisement call and description of the tadpole of Microhyla pulchra Hallowell, 1861 from Dong Nai Culture and Nature Reserve. In: Proceedings of the 3nd National Scientific Workshop “Amphibians and Reptiles in Vietnam”, Hanoi: Publishing House for Science and Technology, 160–168.
    null Mason F . 1860. Burmah, its People and Natural Productions. London: Publ. Trubner, 480.
    null Matsui M . 2011. Taxonomic revision of one of the Old World's smallest frogs, with description of a new bornean Microhyla (Amphibia, Microhylidae). Zootaxa, 2814(1): 33–49.
    null Matsui M , Hamidy A , Belabut DM , Ahmad N , Panha S , Sudin A , Khonsue W , Oh HS , Yong HS , Jiang JP , Nishikawa K . 2011. Systematic relationships of oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. Molecular Phylogenetics and Evolution, 61(1): 167–176.
    null Matsui M , Hamidy A , Eto K . 2013. Description of a new species of Microhyla from Bali, Indonesia (Amphibia, Anura). Zootaxa, 3670(4): 579–590.
    null Matsui M , Ito H , Shimada T , Ota H , Saidapur SK , Khonsue W , Tanaka-Ueno T , Wu GF . 2005. Taxonomic relationships within the pan-oriental narrow-mouth toad Microhyla ornata as revealed by mtDNA analysis (Amphibia, Anura, Microhylidae). Zoological Science, 22(4): 489–495.
    null Matsui M , Shimada T , Liu WZ , Maryati M , Khonsue W , Orlov N . 2006. Phylogenetic relationships of oriental torrent frogs in the genus Amolops and its allies (Amphibia, Anura, Ranidae). Molecular Phylogenetics and Evolution, 38(3): 659–666.
    null Mulcahy DG , Lee JL , Miller AH , Chand M , Thura MK , Zug GR . 2018. Filling the BINs of life: Report of an amphibian and reptile survey of the Tanintharyi (Tenasserim) Region of Myanmar, with DNA barcode data. ZooKeys, 757: 85–152.
    null Nguyen LT , Poyarkov NA Jr, Nguyen TT , Nguyen TA , Nguyen VH , Gorin VA , Murphy RW , Nguyen SN . 2019. A new species of the genus Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) from Tay Nguyen Plateau, Central Vietnam. Zootaxa, 4543(4): 549–580.
    null Palumbi S R , Martin A , Romano S , McMillan W O , Stice L , Grabowski G . 1991. The Simple Fool’s Guide to PCR, Version 2.
    null Privately published document compiled by S . Palumbi, Department of Zoology, University of Hawaii, Honolulu, 96822. .
    null Parker HW . 1928. LXIII. — The brevicipitid frogs of the genus Microhyla. Journal of Natural History, 2(11): 473–499.
    null Parker HW . 1934. Monograph of the Frogs of the Family Microhylidae. London: Trustees of the British Museum, 208.
    null Parker HW , Osman HWC . 1948. LI. — Frogs of the genus Microhyla from Ceylon. Journal of Natural History, 1(10): 759–764.
    null Pawangkhanant P , Poyarkov NA Jr, Duong TV , Naiduangchan M , Suwannapoom C . 2018. A new species of Leptobrachium (Anura, Megophryidae) from western Thailand. PeerJ, 6: e5584.
    null Peel MC , Finlayson BL , McMahon TA . 2007. Updated world map of the K?ppen–Geiger climate classification. Hydrology and Earth System Sciences Discussions, 11: 1633–1644.
    null Peloso PL , Frost DR , Richards SJ , Rodrigues MT , Donnellan S , Matsui M , Raxworthy CJ , Biju S , Lemmon EM , Lemmon AR , Wheeler WC . 2016. The impact of anchored phylogenomics and taxon sampling on phylogenetic inference in narrow-mouthed frogs (Anura, Microhylidae). Cladistics, 32(2): 113–140.
    null Pillai R . 1977. On two frogs of the family Microhylidae from Andamans including a new species. Proceedings of the Indian Academy of Sciences, Section-B, 86(2): 135–138.
    null Posada D , Crandall KA . 1998. Modeltest: testing the model of DNA substitution. Bioinformatics, 14(9): 817–818.
    null Poyarkov NA, Nguyen TV , Duong TV , Gorin VA , Yang JH . 2018. A new limestone-dwelling species of Micryletta (Amphibia: Anura: Microhylidae) from northern Vietnam. PeerJ, 6: e5771.
    null Poyarkov NA Jr, Vassilieva AB , Orlov NL , Galoyan EA , Tran D , Le DTT , Kretova VD , Geissler P . 2014. Taxonomy and distribution of narrow-mouth frogs of the genus Microhyla Tschudi, 1838 (Anura: Microhylidae) from Vietnam with descriptions of five new species. Russian Journal of Herpetology, 21(2): 89–148.
    null Rakotoarison A , Scherz MD , Glaw F , K?hler J , Andreone F , Franzen M , Glos J , Hawlitschek O , Jono T , Mori A , Ndriantsoa SH , Raminosoa NR , Riemann JC , R?del MO, Rosa GM , Vieites DR , Crottini A , Vences M . 2017. Describing the smaller majority: integrative taxonomy reveals twenty-six new species of tiny microhylid frogs (genus Stumpffia) from Madagascar. Vertebrate Zoology, 67(3): 271–398.
    null Rambaut A , Suchard M , Xie W , Drummond A . 2014. Tracer v. 1.6. Institute of Evolutionary Biology, University of Edinburgh. Available at: http: //tree.bio.ed.ac.uk/software/tracer/.
    null Ronquist F , Huelsenbeck JP . 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12): 1572–1574.
    null Sambrook J , Russell DW . 2001. Molecular Cloning: A Laboratory Manual (3rd edition). New York: Cold Spring Harbor Laboratory Press.
    null Sano N , Kurabayashi A , Fujii T , Yonekawa H , Sumida M . 2005. Complete nucleotide sequence of the mitochondrial genome of Schlegel’s tree frog Rhacophorus schlegelii (family Rhacophoridae): duplicated control regions and gene rearrangements. Genes & Genetic Systems, 80(3): 213–224.
    null Savage JM . 1975. Systematics and distribution of the Mexican and Central American stream frogs related to Eleutherodactylus rugulosus . Copeia, 1975(2): 254–306.
    null Schenkel E . 1901. Achter Nachtrag zum Katalog der herpetologischen Sammlung des Basler Museums. Verhandlungen der Naturforschenden Gesellschaft in Basel, 13(1): 142–199. (in German).
    null Seshadri KS , Singal R , Priti H , Ravikanth G , Vidisha MK , Saurabh S , Pratik M , Gururaja KV . 2016. Microhyla laterite sp. nov., a new species of Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) from a laterite rock formation in South West India. PLoS One, 11(3): e0149727.
    null Sheridan JA , Stuart BL . 2018. Hidden species diversity in Sylvirana nigrovittata (Amphibia: Ranidae) highlights the importance of taxonomic revisions in biodiversity conservation. PLoS One, 13(3): e0192766.
    null Shreve B . 1940. Reptiles and amphibians from Burma with descriptions of three new skincs. Proceedings of the New England Zo?logical Club, 18: 17–26.
    null Smith M . 1923. Notes on reptiles and batrachians from Siam and Indo-China (No. 2). Journal of the Natural History Society of Siam, 6(1): 47–53.
    null Solovyeva EN , Lebedev VS , Dunayev EA , Nazarov RA , Bannikova AA , Che J , Murphy RW , Poyarkov NA . 2018. Cenozoic aridization in Central Eurasia shaped diversification of toad-headed agamas (Phrynocephalus; Agamidae, Reptilia). PeerJ, 6: e4543.
    null Stejneger L . 1901. Diagnoses of eight new batrachians and reptiles from the Riu Kiu Archipelago, Japan. Proceedings of the Biological Society of Washington, 14: 189–191.
    null Suwannapoom C , Yuan ZY , Chen JM , Hou M , Zhao HP , Wang LJ , Nguyen TQ , Murphy RW , Sullivan J , McLeod DS , Che J . 2016. Taxonomic revision of the Chinese Limnonectes (Anura, Dicroglossidae) with the description of a new species from China and Myanmar. Zootaxa, 4093(2): 181–200.
    null Tamura K , Stecher G , Peterson D , Filipski A , Kumar S . 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12): 2725–2729.
    null Taylor EH . 1934. Zoological results of the third De Schauensee Siamese Expedition, Part III. Amphibians and reptiles. Proceedings of the Academy of Natural Sciences of Philadelphia, 86: 281–310.
    null Tschudi JJV . 1838. Classification der Batrachier mit Berücksichtigung der fossilen Thiere dieser Abtheilung der Reptilien. Neuchatel: Petitpierre, 124. (in German).
    null Vences M , Thomas M , Bonett RM , Vieites DR . 2005a. Deciphering amphibian diversity through DNA barcoding: chances and challenges. Philosophical Transactions of the Royal Society of London B: Biological Sciences, 360(1462): 1859–1868.
    null Vences M , Thomas M , Van der Meijden A , Chiari Y , Vieites DR . 2005b. Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Frontiers in Zoology, 2(1): 5.
    null Vieites DR , Wollenberg KC , Andreone F , K?hler J , Glaw F , Vences M . 2009. Vast underestimation of Madagascar's biodiversity evidenced by an integrative amphibian inventory. Proceedings of the National Academy of Sciences, 106(20): 8267–8272.
    null Vineeth KK , Radhakrishna U , Godwin R. , Anwesha S , Rajashekhar KP , Aravind N . 2018. A new species of Microhyla Tschudi, 1838 (Anura: Microhylidae) from West Coast of India: an integrative taxonomic approach. Zootaxa, 4420(2): 151–179.
    null Vogt T . 1911. Beitrag zur Amphibien-fauna der Insel Formosa. Sitzungsberichte der Gesellschaft Naturforschender Freunde zu Berlin, 1911: 179–184. (in German)
    null Wijayathilaka N , Garg S , Senevirathne G , Karunarathna N , Biju S , Meegaskumbura M . 2016. A new species of Microhyla (Anura: Microhylidae) from Sri Lanka: an integrative taxonomic approach. Zootaxa, 4066(3): 331–342.
    null Wijayathilaka N , Meegaskumbura M . 2016. An acoustic analysis of the genus Microhyla (Anura: Microhylidae) of Sri Lanka. PLoS One, 11(7): e0159003.
    null Wilkinson JA , Drewes RC , Tatum OL . 2002. A molecular phylogenetic analysis of the family Rhacophoridae with an emphasis on the Asian and African genera. Molecular Phylogenetics and Evolution, 24(2): 265–273.
    null Wilkinson JA , Sellas AB , Vindum JV . 2012. A new species of Ansonia (Anura: Bufonidae) from northern Tanintharyi Division, Myanmar. Zootaxa, 3163: 54–68.
    null Wilkinson JA , Win H , Thin T , Lwin KS , Shein AK , Tun H . 2003. A new species of Chirixalus (Anura: Rhacophoridae) from western Myanmar (Burma). Proceedings of the California Academy of Sciences, 54: 17–26.
    null Wilkinson M , Presswell B , Sherratt E , Papadopoulou A , Gower DJ . 2014. A new species of striped Ichthyophis Fitzinger, 1826 (Amphibia: Gymnophiona: Ichthyophiidae) from Myanmar. Zootaxa, 3785(1): 45–58.
    null Wogan GOU . 2012. A new species of Leptobrachium from Myanmar (Anura: Megophryidae). Zootaxa, 3415: 23–36.
    null Wogan GOU, Vindum JV , Wilkinson JA , Koo MS , Slowinski JB , Win H , Thin T , Kyi SW , Oo SL , Lwin KS , Shein AK . 2008. New country records and range extensions for Myanmar amphibians and reptiles. Hamadryad, 33: 83–96.
    null Wogan GOU, Win H , Thin T , Lwin KS , Shein AK , Kyi SW , Tun H . 2003. A new species of Bufo (Anura: Bufonidae) from Myanmar (Burma), and redescription of the little-known species Bufo stuarti Smith, 1929. Proceedings of the California Academy of Sciences, 54: 141–153.
    null Yuan ZY , Suwannapoom C , Yan F , Poyarkov NA, Nguyen SN , Chen HM , Chomdej S , Murphy RW , Che J . 2016. Red River barrier and Pleistocene climatic fluctuations shaped the genetic structure of Microhyla fissipes complex (Anura: Microhylidae) in southern China and Indochina. Current Zoology, 62(6): 531–543.
    null Zaw T , Lay P , Pawangkhanant P , Gorin VA , Poyarkov NA . 2019. A new species of Crocodile Newt, genus Tylototriton (Amphibia, Caudata, Salamandridae) from mountains of Kachin State, northern Myanmar. Zoological Research, 40(3): 151–174.
    null Zhang M , Fei L , Ye C , Wang Y , Wang B , Jiang J . 2018. A new species of genus Microhyla (Amphibia: Anura: Microhylidae) from Zhejiang Province, China. Asian Herpetological Research, 9(3): 135–148.
    null Zug GR . 2015. Morphology and systematics of Kalophrynus interlineatus–pleurostigma populations (Anura: Microhylidae: Kalphryninae) and a taxonomy of the genus Kalophrynus Tschudi, Asian Sticky Frogs. Proceedings of the California Academy of Sciences, 62: 135–190.
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On the road to Mandalay: contribution to the Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) fauna of Myanmar with description of two new species

doi: 10.24272/j.issn.2095-8137.2019.044
Funds:  This study was partially supported by the Russian Science Foundation (19-14-00050) to N.A.P., Southeast Asia Biodiversity Research Institute, CAS (Y4ZK111B01: 2017CASSEAB RIQG002), and Animal Branch of the Germplasm Bank of Wild Species, CAS(Large Research Infrastructure Funding)
    Corresponding author: Nikolay A. Poyarkov Jr.

Abstract: We present a morphological and molecular assessment of the Microhyla fauna of Myanmar based on new collections from central (Magway Division) and northern (Kachin State) parts of the country. In total, six species of Microhyla are documented, including M. berdmorei, M. heymonsi, M. butleri, M. mukhlesuri and two new species described from the semi-arid savanna-like plains of the middle part of the Irrawaddy (Ayeyarwady) River Valley. We used a 2 481 bp long 12S rRNA–16S rRNA fragment of mtDNA to hypothesize genealogical relationships within Microhyla. We applied an integrative taxonomic approach combining molecular, morphological, and acoustic lines of evidence to evaluate the taxonomic status of Myanmar Microhyla. We demonstrated that the newly discovered populations of Microhyla sp. from the Magway Division represent two yet undescribed species. These two new sympatric species are assigned to the M. achatina species group, with both adapted to the seasonally dry environments of the Irrawaddy Valley. Microhyla fodiens sp.nov. is a stout-bodied species with a remarkably enlarged shovel-like outer metatarsal tubercle used for burrowing and is highly divergent from other known congeners (P-distance≥8.8%). Microhyla irrawaddy sp. nov. is a small-bodied slender frog reconstructed as a sister species to M. kodial from southern India (P-distance=5.3%); however, it clearly differs from the latter both in external morphology and advertisement call parameters. Microhyla mukhlesuri is reported from Myanmar for the first time. We further discuss the morphological diagnostics and biogeography of Microhyla species recorded in Myanmar.

Nikolay A. Poyarkov Jr., Vladislav A. Gorin, Than Zaw, Valentina D. Kretova, Svetlana S. Gogoleva, Parinya Pawangkhanant, Jing Che. On the road to Mandalay: contribution to the Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) fauna of Myanmar with description of two new species. Zoological Research, 2019, 40(4): 244-276. doi: 10.24272/j.issn.2095-8137.2019.044
Citation: Nikolay A. Poyarkov Jr., Vladislav A. Gorin, Than Zaw, Valentina D. Kretova, Svetlana S. Gogoleva, Parinya Pawangkhanant, Jing Che. On the road to Mandalay: contribution to the Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) fauna of Myanmar with description of two new species. Zoological Research, 2019, 40(4): 244-276. doi: 10.24272/j.issn.2095-8137.2019.044
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