Please wait a minute...
ZOOLOGICAL RESEARCH    2016, Vol. 37 Issue (4) : 246-251     DOI: 10.13918/j.issn.2095-8137.2016.4.246
Articles |
Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina)
Xiao-Liang ZHANG1,3, Jia-Hao SONG1,2, Wei PANG1, Yong-Tang ZHENG1,3,4
1 Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;
2 Institute of Health Sciences, Anhui University, Hefei Anhui 230601, China;
3 Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China;
4 Kunming Primate Research Center of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China
Download: PDF(1743 KB)   RICH HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks     Supplementary material
Abstract  

Northern pig-tailed macaques (NPMs, Macaca leonina) are susceptible to HIV-1 infection largely due to the loss of HIV-1-restricting factor TRIM5α. However, great impediments still exist in the persistent replication of HIV-1 in vivo, suggesting some viral restriction factors are reserved in this host. The APOBEC3 proteins have demonstrated a capacity to restrict HIV-1 replication, but their inhibitory effects in NPMs remain elusive. In this study, we cloned the NPM A3A-A3H genes, and determined by BLAST searching that their coding sequences (CDSs) showed 99% identity to the corresponding counterparts from rhesus and southern pig-tailed macaques. We further analyzed the anti-HIV-1 activities of the A3A-A3H genes, and found that A3G and A3F had the greatest anti-HIV-1 activity compared with that of other members. The results of this study indicate that A3G and A3F might play critical roles in limiting HIV-1 replication in NPMs in vivo. Furthermore, this research provides valuable information for the optimization of monkey models of HIV-1 infection.

Keywords Macaca leonina      Northern pig-tailed macaques      APOBEC3      HIV     
Fund:

This work was supported by the National Special Science Research Program of China (2012CBA01305), National Natural Science Foundation of China (81172876; 81471620; 81273251; 81571606; U0832601), National Science and Technology Major Project (2014ZX10005-002-006), Knowledge Innovation Program of CAS (KJZD-EW-L10-02) and Yunnan Applicative and Basic Research Program (2014FB181)

Corresponding Authors: Xiao-Liang ZHANG   
Service
E-mail this article
E-mail Alert
RSS
Articles by authors
Xiao-Liang ZHANG
Jia-Hao SONG
Wei PANG
Yong-Tang ZHENG
Cite this article:   
Xiao-Liang ZHANG,Jia-Hao SONG,Wei PANG, et al. Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina)[J]. ZOOLOGICAL RESEARCH, 2016, 37(4): 246-251.
URL:  
http://www.zoores.ac.cn/EN/10.13918/j.issn.2095-8137.2016.4.246     OR     http://www.zoores.ac.cn/EN/Y2016/V37/I4/246
Agy MB, Frumkin LR, Corey L, Coombs RW, Wolinsky SM, Koehler J, Morton WR, Katze MG. 1992. Infection of Macaca nemestrina by human immunodeficiency virus type-1. Science, 257(5066):103-106.
Belshaw R, Pereira V, Katzourakis A, Talbot G, Pa?es J, Burt A, Tristem M. 2004. Long-term reinfection of the human genome by endogenous retroviruses. Proceedings of the National Academy of Sciences of the United States of America, 101(14):4894-4899.
Bosch ML, Schmidt A, Chen JL, Florey MJ, Agy M, Morton WR. 2000. Enhanced replication of HIV-1 in vivo in pigtailed macaques (Macaca nemestrina). Journal of Medical Primatology, 29(3-4):107-113.
Dai ZX, Zhang GH, Zhang XH, Zheng YT. 2013. Identification and characterization of a novel splice variant of rhesus macaque MHC IA. Molecular Immunology, 53(3):206-213.
Groves CP. 2001. Primate Taxonomy. Washington DC:Smithsonian Institution Press, 222-224.
Hatziioannou T, Evans DT. 2012. Animal models for HIV/AIDS research. Nature Reviews Microbiology, 10(12):852-867.
Hatziioannou T, Del Prete GQ, Keele BF, Estes JD, Mcnatt MW, Bitzegeio J, Raymond A, Rodriguez A, Schmidt F, Mac Trubey C, Smedley J, Piatak M Jr, Kewalramani VN, Lifson JD, Bieniasz PD. 2014. HIV-1-induced AIDS in monkeys. Science, 344(6190):1401-1405.
Hu SL. 2005. Non-human primate models for AIDS vaccine research. Current Drug Targets-Infectious Disorders, 5(2):193-201.
Jarmuz A, Chester A, Bayliss J, Gisbourne J, Dunham I, Scott J, Navaratnam N. 2002. An anthropoid-specific locus of orphan C to U RNA-editing enzymes on chromosome 22. Genomics, 79(3):285-296.
Jia XF, Zhao Q, Xiong Y. 2015. HIV suppression by host restriction factors and viral immune evasion. Current Opinion in Structural Biology, 31:106-114.
Kuang YQ, Tang X, Liu FL, Jiang XL, Zhang YP, Gao G, Zheng YT. 2009. Genotyping of TRIM5 locus in northern pig-tailed macaques (Macaca leonina), a primate species susceptible to Human Immunodeficiency Virus type 1 infection. Retrovirology, 6:58.
Lei AH, Zhang GH, Tian RR, Zhu JW, Zheng HY, Pang W, Zheng YT. 2014. Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina). Zoological Research, 35(3):186-195.
Lian XD, Zhang XH, Dai ZX, Zheng YT. 2016. Cloning, sequencing, and polymorphism analysis of novel classical MHC class I alleles in northern pig-tailed macaques (Macaca leonina). Immunogenetics, 68(4):261-274.
Liao CH, Kuang YQ, Liu HL, Zheng YT, Su B. 2007. A novel fusion gene, TRIM5-Cyclophilin A in the pig-tailed macaque determines its susceptibility to HIV-1 infection. AIDS, 21 Suppl 8:S19-S26.
Pang W, Lü LB, Wang Y, Li G, Huang DT, Lei AH, Zhang GH, Zheng YT. 2013. Measurement and analysis of hematology and blood chemistry parameters in northern pig-tailed macaques (Macaca leonina). Zoological Research, 34(2):89-96. (in Chinese)
Pillai SK, Abdel-Mohsen M, Guatelli J, Skasko M, Monto A, Fujimoto K, Yukl S, Greene WC, Kovari H, Rauch A, Fellay J, Battegay M, Hirschel B, Witteck A, Bernasconi E, Ledergerber B, Gunthard HF, Wong JK. 2012. Role of retroviral restriction factors in the interferon-alpha-mediated suppression of HIV-1 in vivo. Proceedings of the National Academy of Sciences of the United States of America, 109(8):3035-3040.
Prohaska KM, Bennett RP, Salter JD, Smith HC. 2014. The multifaceted roles of RNA binding in APOBEC cytidine deaminase functions. Wiley Interdisciplinary Reviews:RNA, 5(4):493-508.
Sawyer SL, Emerman M, Malik HS. 2004. Ancient adaptive evolution of the primate antiviral DNA-editing enzyme APOBEC3G. PLoS Biology, 2(9):e275.
Simon V, Bloch N, Landau NR. 2015. Intrinsic host restrictions to HIV-1 and mechanisms of viral escape. Nature Immunology, 16(6):546-553.
Stavrou S, Ross SR. 2015. APOBEC3 proteins in viral immunity. The Journal of Immunology, 195(10):4565-4570.
Stenglein MD, Burns MB, Li M, Lengyel J, Harris RS. 2010. APOBEC3 proteins mediate the clearance of foreign DNA from human cells. Nature Structural & Molecular Biology, 17(2):222-229.
Suspène R, Guétard D, Henry M, Sommer P, Wain-Hobson S, Vartanian JP. 2005. Extensive editing of both hepatitis B virus DNA strands by APOBEC3 cytidine deaminases in vitro and in vivo. Proceedings of the National Academy of Sciences of the United States of America, 102(23):8321-8326.
Vartanian JP, Guetard D, Henry M, Wain-Hobson S. 2008. Evidence for editing of human papillomavirus DNA by APOBEC3 in benign and precancerous lesions. Science, 320(5873):230-233.
Virgen CA, Hatziioannou T. 2007. Antiretroviral activity and Vif sensitivity of rhesus macaque APOBEC3 proteins. Journal of Virology, 81(24):13932-13937.
Wedekind JE, Dance GS, Sowden MP, Smith HC. 2003. Messenger RNA editing in mammals:new members of the APOBEC family seeking roles in the family business. Trends in Genetics, 19(4):207-216.
Wolfe ND, Switzer WM, Carr JK, Bhullar VB, Shanmugam V, Tamoufe U, Prosser AT, Torimiro JN, Wright A, Mpoudi-Ngole E, Mccutchan FE, Birx DL, Folks TM, Burke DS, Heneine W. 2004. Naturally acquired simian retrovirus infections in central African hunters. The Lancet, 363(9413):932-937.
Zennou V, Bieniasz PD. 2006. Comparative analysis of the antiretroviral activity of APOBEC3G and APOBEC3F from primates. Virology, 349(1):31-40.
Zhang GH, Li MH, Zheng YT. 2007. Application of AIDS macaque animal model in HIV vaccine research. Zoological Research, 28(5):556-562.
Zhang JZ, Webb DM. 2004. Rapid evolution of primate antiviral enzyme APOBEC3G. Human Molecular Genetics, 13(16):1785-1791.
Zhang MX, Zheng HY, Jiang J, Pang W, Zhang GH, Zheng YT. 2016. Viral seroprevalence in northern pig-tailed macaques (Macaca leonina) derived from Ho Chi Minh City, Vietnam. Primates, doi:10.1007/s10329-016-0531-5.
Zhang XL, Pang W, Deng DY, Lv LB, Feng Y, Zheng YT. 2014. Analysis of immunoglobulin, complements and CRP levels in serum of captive northern pig-tailed macaques (Macaca leonina). Zoological Research, 35(3):196-203.
Zheng HY, Zhang MX, Zhang LT, Zhang XL, Pang W, Lyu LB, Zheng YT. 2014. Flow cytometric characterizations of leukocyte subpopulations in the peripheral blood of northern pig-tailed macaques (Macaca leonina). Zoological Research, 35(6):465-473.
Zhu L, Lei AH, Zheng HY, Lyu LB, Zhang ZG, Zheng YT. 2015. Longitudinal analysis reveals characteristically high proportions of bacterial vaginosis-associated bacteria and temporal variability of vaginal microbiota in northern pig-tailed macaques (Macaca leonina). Zoological Research, 36(5):285-298.
[1] Dong Li, Feng-Jie Wang, Lei Yu, Wen-Rong Yao, Yan-Fang Cui, Gui-Bo Yang. Expression of pIgR in the tracheal mucosa of SHIV/SIV-infected rhesus macaques[J]. ZOOLOGICAL RESEARCH, 2017, 38(1): 44-48.
[2] Lin ZHANG, Xu-He HUANG, Ping-Ping ZHOU, Guo-Long YU, Jin YAN, Bing QIN, Xin-Ge YAN, Li-Mei DIAO, Peng LIN, Yi-Qun KUANG. Establishment of HIV-1 model cell line GHOST(3) with stable DRiP78 and NHERF1 knockdown[J]. ZOOLOGICAL RESEARCH, 2015, 36(3): 161-166.
[3] Ai-Hua LEI, Gao-Hong ZHANG, Ren-Rong TIAN, Jia-Wu ZHU, Hong-Yi ZHENG, Wei PANG, Yong-Tang ZHENG. Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina)[J]. ZOOLOGICAL RESEARCH, 2014, 35(3): 186-195.
[4] Ming GUO, Wen-Zhe HO. Animal models to study Mycobacterium tuberculosis and HIV co-infection[J]. ZOOLOGICAL RESEARCH, 2014, 35(3): 163-169.
[5] Shuai TIAN, Yu-Huan MENG, Ming-Yu LIU, Fei SUN, Jun-Hui CHEN, Hong-Li DU, Xiao-Ning WANG. TRIMCyp frequency of the cynomolgus macaque (Macaca fascicularis) in captivity in China[J]. ZOOLOGICAL RESEARCH, 2013, 34(2): 97-102.
[6] Wei PANG, Long-Bao LÜ, Yun WANG, Gui LI, Dong-Ti HUANG, Ai-Hua LEI, Gao-Hong ZHANG, Yong-Tang ZHENG. Measurement and analysis of hematology and blood chemistry parameters in northern pig-tailed macaques (Macaca leonina)[J]. ZOOLOGICAL RESEARCH, 2013, 34(2): 89-96.
[7] Ai-Hua LEI, Wei PANG, Gao-Hong ZHANG, Yong-Tang ZHENG. Use and research of pigtailed macaques in nonhuman primate HIV/AIDS models[J]. ZOOLOGICAL RESEARCH, 2013, 34(2): 77-88.
[8] LI Pei-Lu, CHEN Qian-Qian, ZHANG Chi-Yu. SAMHD1— A HIV-1 restriction factor derived from myeloid lineage monocytes[J]. ZOOLOGICAL RESEARCH, 2012, 33(5): 537-541.
[9] CAO Guang, LIU Feng-Liang, ZHANG Gao-Hong, ZHENG Yong-Tang. The primate TRIMCyp fusion genes and mechanism of restricting retroviruses replication[J]. ZOOLOGICAL RESEARCH, 2012, 33(1): 99-107.
[10] CAO Guang,NIE Wen-Hui,LIU Feng-Liang,KUANG Yi-Qun,WANG Jin-Huan,SU Wei-Ting,ZH Y. Identification of the TRIM5/TRIMCyp heterozygous genotype in Macaca assamensis[J]. ZOOLOGICAL RESEARCH, 2011, 32(1): 40-49.
[11] ZHU Lin,ZHANG Gao-Hong,ZHENG Yong-Tang. Application Studies of Animal Models in Evaluating Safety and Efficacy of HIV-1 Microbicides[J]. ZOOLOGICAL RESEARCH, 2010, 31(1): 66-76.
[12] HANG Gao-hong, LI Ming-hua, ZHENG Yong-tang. Application of AIDS Macaque Animal Model in HIV Vaccine Research[J]. Zoological Research, 2007, 28(5): 556-562.
[13] WANG Jian-hua ,,OUYANG Dong-yun,WANG Yuan-yuan,ZHENG Yong-tang ,*. Progress in Anti-HIV-1 Actions of Ribosome Inactivating Proteins[J]. ZOOLOGICAL RESEARCH, 2006, 27(5): 541-548.
[14] ZHANG Gao-hong,CHEN Ya-li,TANG Hong,ZHENG Yong-tang. Humanized SCID Mouse:A Small Animal Model for HIV Research[J]. ZOOLOGICAL RESEARCH, 2004, 25(4): 356-362.
[15] ZHANG Chi-yu. Phenotype and Cellular Tropism of Human Immunodeficiency Virus Type 1[J]. ZOOLOGICAL RESEARCH, 2004, 25(4): 363-368.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed