Zoological Research ›› 2016, Vol. 37 ›› Issue (4): 246-251.doi: 10.13918/j.issn.2095-8137.2016.4.246

• Articles • Previous Articles     Next Articles

Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina)

Xiao-Liang ZHANG1,3, Jia-Hao SONG1,2, Wei PANG1, Yong-Tang ZHENG1,3,4   

  1. 1 Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;
    2 Institute of Health Sciences, Anhui University, Hefei Anhui 230601, China;
    3 Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China;
    4 Kunming Primate Research Center of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China
  • Received:2016-05-23 Revised:2016-07-05 Online:2016-07-18 Published:2016-07-18
  • Contact: Xiao-Liang ZHANG E-mail:zhengyt@mail.kiz.ac.cn
  • Supported by:

    This work was supported by the National Special Science Research Program of China (2012CBA01305), National Natural Science Foundation of China (81172876; 81471620; 81273251; 81571606; U0832601), National Science and Technology Major Project (2014ZX10005-002-006), Knowledge Innovation Program of CAS (KJZD-EW-L10-02) and Yunnan Applicative and Basic Research Program (2014FB181)

Abstract:

Northern pig-tailed macaques (NPMs, Macaca leonina) are susceptible to HIV-1 infection largely due to the loss of HIV-1-restricting factor TRIM5α. However, great impediments still exist in the persistent replication of HIV-1 in vivo, suggesting some viral restriction factors are reserved in this host. The APOBEC3 proteins have demonstrated a capacity to restrict HIV-1 replication, but their inhibitory effects in NPMs remain elusive. In this study, we cloned the NPM A3A-A3H genes, and determined by BLAST searching that their coding sequences (CDSs) showed 99% identity to the corresponding counterparts from rhesus and southern pig-tailed macaques. We further analyzed the anti-HIV-1 activities of the A3A-A3H genes, and found that A3G and A3F had the greatest anti-HIV-1 activity compared with that of other members. The results of this study indicate that A3G and A3F might play critical roles in limiting HIV-1 replication in NPMs in vivo. Furthermore, this research provides valuable information for the optimization of monkey models of HIV-1 infection.

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