Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (<em>Macaca leonina</em>)
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ZOOLOGICAL RESEARCH    2016, Vol. 37 Issue (4) : 246-251     DOI: 10.13918/j.issn.2095-8137.2016.4.246
Articles |
Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina)
Xiao-Liang ZHANG1,3, Jia-Hao SONG1,2, Wei PANG1, Yong-Tang ZHENG1,3,4
1 Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;
2 Institute of Health Sciences, Anhui University, Hefei Anhui 230601, China;
3 Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China;
4 Kunming Primate Research Center of the Chinese Academy of Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China
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Northern pig-tailed macaques (NPMs, Macaca leonina) are susceptible to HIV-1 infection largely due to the loss of HIV-1-restricting factor TRIM5α. However, great impediments still exist in the persistent replication of HIV-1 in vivo, suggesting some viral restriction factors are reserved in this host. The APOBEC3 proteins have demonstrated a capacity to restrict HIV-1 replication, but their inhibitory effects in NPMs remain elusive. In this study, we cloned the NPM A3A-A3H genes, and determined by BLAST searching that their coding sequences (CDSs) showed 99% identity to the corresponding counterparts from rhesus and southern pig-tailed macaques. We further analyzed the anti-HIV-1 activities of the A3A-A3H genes, and found that A3G and A3F had the greatest anti-HIV-1 activity compared with that of other members. The results of this study indicate that A3G and A3F might play critical roles in limiting HIV-1 replication in NPMs in vivo. Furthermore, this research provides valuable information for the optimization of monkey models of HIV-1 infection.

Keywords Macaca leonina      Northern pig-tailed macaques      APOBEC3      HIV     

This work was supported by the National Special Science Research Program of China (2012CBA01305), National Natural Science Foundation of China (81172876; 81471620; 81273251; 81571606; U0832601), National Science and Technology Major Project (2014ZX10005-002-006), Knowledge Innovation Program of CAS (KJZD-EW-L10-02) and Yunnan Applicative and Basic Research Program (2014FB181)

Corresponding Authors: Xiao-Liang ZHANG   
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Xiao-Liang ZHANG,Jia-Hao SONG,Wei PANG, et al. Molecular cloning and anti-HIV-1 activities of APOBEC3s from northern pig-tailed macaques (Macaca leonina)[J]. ZOOLOGICAL RESEARCH, 2016, 37(4): 246-251.
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