Zoological Research ›› 2016, Vol. 37 ›› Issue (4): 205-213.doi: 10.13918/j.issn.2095-8137.2016.4.205

Special Issue: Animal models Genetics & evolution

• Reviews • Previous Articles     Next Articles

Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems

Li-Fang JIN1,2,3, Jin-Song LI2,3   

  1. 1 College of Life Science of Shaoxing University, Shaoxing Zhejiang 312000, China;
    2 State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China;
    3 Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China
  • Received:2016-04-11 Revised:2016-05-05 Online:2016-07-18 Published:2016-06-27
  • Contact: Li-Fang JIN E-mail:Lifangj@sohu.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (3731530048C1202)

Abstract: With the development of high-throughput sequencing technology in the post-genomic era, researchers have concentrated their efforts on elucidating the relationships between genes and their corresponding functions. Recently, important progress has been achieved in the generation of genetically modified mice based on CRISPR/Cas9 and haploid embryonic stem cell (haESC) approaches, which provide new platforms for gene function analysis, human disease modeling, and gene therapy. Here, we review the CRISPR/Cas9 and haESC technology for the generation of genetically modified mice and discuss the key challenges in the application of these approaches.