Please wait a minute...
ZOOLOGICAL RESEARCH    2016, Vol. 37 Issue (1) : 31-40     DOI: 10.13918/j.issn.2095-8137.2016.1.31
Articles |
A new species of the genus Amolops (Amphibia: Ranidae) from southeastern Tibet, China
Ke JIANG1, Kai WANG1,2, Fang YAN1, Jiang XIE3, Da-Hu ZOU4,1, Wu-Lin LIU5, Jian-Ping JIANG6, Cheng LI7, Jing CHE1
1 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223, China;
2 Sam Noble Oklahoma Museum of Natural History and Department of Biology, University of Oklahoma, Norman OK 73072-7029, U.S.A.;
3 North China Electric Power University Science and Technology College, Baoding Hebei 071051, China;
4 Tibet University, Lhasa Tibet 850000, China;
5 Institute of Forestry Survey, Plan and Research of Xizang Autonomous Region, Lhasa Tibet 850000, China;
6 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu Sichuan 610041, China;
7 Imaging Biodiversity Expedition, Beijing 100107, China
Download: PDF(4024 KB)   RICH HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks    

A new species of the genus Amolops Cope, 1865 is described from Nyingchi, southeastern Tibet, China, based on morphological and molecular data. The new species, Amolops nyingchiensis sp. nov. is assigned to the Amolops monticola group based on its skin smooth, dorsolateral fold distinct, lateral side of head black, upper lip stripe white extending to the shoulder. Amolops nyingchiensis sp. nov. is distinguished from all other species of Amolops by the following combination of characters: (1) medium body size, SVL 48.5-58.3 mm in males, and 57.6-70.7 mm in females; (2) tympanum distinct, slightly larger than one third of the eye diameter; (3) a small tooth-like projection on anteromedial edge of mandible; (4) the absence of white spine on dorsal surface of body; (5) the presence of circummarginal groove on all fingers; (6) the presence of vomerine teeth; (7) background coloration of dorsal surface brown, lateral body gray with yellow; (8) the presence of transverse bands on the dorsal limbs; (9) the presence of nuptial pad on the first finger in males; (10) the absence of vocal sac in males. Taxonomic status of the populations that were previously identified to A. monticola from Tibet is also discussed.

Keywords Amolops monticola group      Amolops nyingchiensis sp. nov.      DNA barcoding      Tibet     

This study was supported by the Ministry of Science and Technology of China (2014FY210200 and 2011FY120200), the Animal Branch of the Germplasm Bank of Wild Species of Chinese Academy of Sciences (the Large Research Infrastructure Funding), and partially supported by the project "National Second Survey of Wild-animals in Xizang Autonomous Region of China".

Corresponding Authors: Jing CHE   
E-mail this article
E-mail Alert
Articles by authors
Cite this article:   
Ke JIANG, Kai WANG, Fang YAN, Jiang XIE, Da-Hu ZOU, Wu-Lin LIU, Jian-Ping JIANG, Cheng LI, Jing CHE. A new species of the genus Amolops (Amphibia: Ranidae) from southeastern Tibet, China. ZOOLOGICAL RESEARCH, 2016, 37(1): 31-40.
URL:     OR
Anderson J. 1871. A list of the reptilian accession to the Indian Museum, Calcutta from 1865 to 1870, with a description of some new species. Journal of the Asiatic Society of Bengal, 40: 12-39.
Boulenger GA. 1920. A monograph of the South Asian, Papuan, Melanesian and Australian frogs of the genus Rana. Records of the Indian Museum, 20: 1-226.
Cai HX, Che J, Pang JF, Zhao EM, Zhang YP. 2007. Paraphyly of Chinese Amolops (Anura, Ranidae) and phylogenetic position of the rare Chinese frog, Amolops tormotus. Zootaxa, 1531: 49-55.
Che J, Chen HM, Yang JX, Jin JQ, Jiang K, Yang ZY, Murphy RW, Zhang YP 2012. Universal COI primers for DNA barcoding amphibians. Molecular Ecology Resources, 12 (2): 247-258.
Fei L, Hu SQ, Ye CY, Huang YZ. 2009a. Fauna Sinica, Amphibia. Vol. 2. Beijing: Science Press, 1-957. (in Chinese)
Fei L, Hu SQ, Ye CY, Huang YZ. 2009b. Fauna Sinica, Amphibia. Vol. 3. Beijing: Science Press, 959-1847. (in Chinese)
Fei L, Hu SQ, Ye CY, Wu GF. 1977. A survey of amphibians in Xizang (Tibet). Acta Zoologica Sinica, 23 (1): 54-63. (in Chinese)
Fei L, Ye CY, Huang YZ. 2005. An illustrated key to Chinese amphibians. Chengdu: Sichuan Publishing Group, 1-340. (in Chinese)
Frost DR. 2015. Amphibian Species of the World: an Online Reference. Version 6.0 (Nov 26, 2015). Electronic Database accessible at American Museum of Natural History, New York, USA.
Hou M, Wu YK, Yang KL, Zheng S, Yuan ZY, Li PP. 2014. A missing geographic link in the distribution of the genus Echinotriton (Caudata: Salamandridae) with description of a new species from southern China. Zootaxa, 3895: 89-102.
Hu SQ. 1987. Amphibia-Reptilia of Xizang. Beijing: Science Press, 1-153. (in Chinese)
Li PP, Zhao EM, Dong BJ. 2010. Amphibians and Reptiles of Tibet. Beijing: Science Press, 1-251. (in Chinese)
Liu WZ, Yang DT, Ferraris C, Matsui M. 2000. Amolops bellulus: A new species of stream-breeding frog from western Yunnan, China (Anura: Ranidae). Copeia, 2000 (2): 535-541.
Matsui M, Shimada T, Liu WZ, Maryati M, Khonsue W, Orlov N. 2006. Phylogenetic relationships of Oriental torrent frogs in the genus Amolops and its allies (Amphibia, Anura, Ranidae). Molecular Phylogenetics and Evolution, 38 (3): 659-666.
Posada D, Crandall KA. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics, 14 (9): 817-818.
Ronquist F, Huelsenbeck JP. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19 (12): 1572-1574.
Sambrook J, Fritsch E, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual (2nd edition). New York: Cold Spring Harbor Laboratory Press.
Savage JM, Heyer WR. 1997. Digital webbing formulae for anurans: a refinement. Herpetological Review, 28 (3): 131.
Stuart BL. 2008. The phylogenetic problem of Huia (Amphibia: Ranidae). Molecular Phylogenetics and Evolution, 46 (1): 49-60.
Stuart BL, Bain RH, Phimmachak S, Spence K. 2010. Phylogenetic systematics of the Amolops monticola group (Amphibia: Ranidae), with description of a new species from northwestern Laos. Herpetologica, 66 (1): 52-66.
Sun GZ, Luo WX, Sun HY, Zhang GY. 2013. A new species of cascade frog from Tibet: China-Amolops chayuensis. Forestry Construction, 5 (1): 14-16. (in Chinese)
Tamura K, Peterson D, Peterson N, Stecher G. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28 (10): 2731-2739.
Wu YK, Murphy RW. 2015. Concordant species delimitation from multiple independent evidence: A case study with the Pachytriton brevipes complex (Caudata: Salamandridae). Molecular Phylogenetic and Evolution, 92: 108-117.
[1] Chun-Xia Wang, Xin Xu, Shu-Qiang Li. Integrative taxonomy of Leptonetela spiders (Araneae, Leptonetidae), with descriptions of 46 new species[J]. Zoological Research, 2017, 38(6): 321-448.
[2] Yi-Ming Hu, Zhi-Xin Zhou, Zhi-Wen Huang, Ming Li, Zhi-Gang Jiang, Jian-Pu Wu, Wu-Lin Liu, Kun Jin, Hui-Jian Hu. A new record of the capped langur (Trachypithecus pileatus) in China[J]. Zoological Research, 2017, 38(4): 203-205.
[3] Yong-Bo Guo, Yao-Xi He, Chao-Ying Cui, Ouzhuluobu, Baimakangzhuo, Duojizhuoma, Dejiquzong, Bianba, Yi Peng, Cai-juan Bai, Gonggalanzi, Yong-Yue Pan, Qula, Kangmin, Cirenyangji, Baimayangji, Wei Guo, Yangla, Hui Zhang, Xiao-Ming Zhang, Wang-Shan Zheng, Shu-Hua Xu, Hua Chen, Sheng-Guo Zhao, Yuan Cai, Shi-Ming Liu, Tian-Yi Wu, Xue-Bin Qi, Bing Su. GCH1 plays a role in the high-altitude adaptation of Tibetans[J]. ZOOLOGICAL RESEARCH, 2017, 38(3): 155-162.
[4] Wang-Shan Zheng, Yao-Xi He, Chao-Ying Cui, Ouzhuluobu, Dejiquzong, Yi Peng, Cai-Juan Bai, Duojizhuoma, Gonggalanzi, Bianba, Baimakangzhuo, Yong-Yue Pan, Qula, Kangmin, Cirenyangji, Baimayangji, Wei Guo, Yangla, Hui Zhang, Xiao-Ming Zhang, Yong-Bo Guo, Shu-Hua Xu, Hua Chen, Sheng-Guo Zhao, Yuan Cai, Shi-Ming Liu, Tian-Yi Wu, Xue-Bin Qi, Bing Su. EP300 contributes to high-altitude adaptation in Tibetans by regulating nitric oxide production[J]. ZOOLOGICAL RESEARCH, 2017, 38(3): 163-170.
[5] Ke JIANG, Kai WANG, Da-Hu ZOU, Fang YAN, Pi-Peng LI, Jing CHE. A new species of the genus Scutiger (Anura: Megophryidae) from Medog of southeastern Tibet, China[J]. ZOOLOGICAL RESEARCH, 2016, 37(1): 21-30.
[6] Jing CHE, Kai WANG. AmphibiaChina: an online database of Chinese Amphibians[J]. ZOOLOGICAL RESEARCH, 2016, 37(1): 57-59.
[7] Guo-Gang ZHANG, Dong-Ping LIU, Feng-Shan LI, Fa-Wen QIAN, Tian MA, Ding DAN, Jun LU . Species and populations of waterbirds wintering in the Yarlung Zangbo and its tributaries in Tibet,China[J]. ZOOLOGICAL RESEARCH, 2014, 35(S1): 92-100.
[8] Qi-Xin ZHANG, Jin-Hua LI, Dong-Po XIA, Yong ZHU, Xi WANG, Dao ZHANG. Influence of dominance rank and affiliation relationships on self-directed behavior in female Tibetan macaques (Macaca thibetana)[J]. ZOOLOGICAL RESEARCH, 2014, 35(3): 214-221.
[9] Shuang WANG, Jin-Hua LI, Dong-Po XIA, Yong ZHU, Bing-Hua SUN, Xi WANG, Lei ZHU. Male-to-female agonistic support for copulation in Tibetan macaques (Macaca thibetana) at Huangshan, China[J]. ZOOLOGICAL RESEARCH, 2013, 34(3): 139-144.
[10] WANG Cheng-Ye, FENG Ying, CHEN Xiao-Ming. DNA barcoding analysis of processed medicinal insect Catharsius molossus[J]. ZOOLOGICAL RESEARCH, 2012, 33(6): 597-602.
[11] SUN Ping,YU Hong-Hao,ZHAO Xin-Quan,WANG De-Hua. Adaptation of vigilance behavior in ex situ conservation of Tibetan antelope[J]. ZOOLOGICAL RESEARCH, 2011, 32(5): 561-565.
[12] XU Ai-Chun,JIANG Zhi-Gang,LI Chun-Wang,CAI Ping. Food habits and hunting patterns of Tibetan brown bear during warm seasons in Kekexili region on Qinghai-Tibetan Plateau[J]. ZOOLOGICAL RESEARCH, 2010, 31(6): 670-674.
[13] XIA Dong-Po,LI Jin-Hua,ZHU Yong,SUN Bing-Hua,Lori K SHEERAN,Megan D MATHESON. Seasonal variation and synchronization of sexual behaviors in free-ranging male Tibetan macaques (Macaca thibetana) at Huangshan, China[J]. ZOOLOGICAL RESEARCH, 2010, 31(5): 509-515.
[14] JI Huan,LI Jin-Hua,SUN Bing-Hua,ZHU Yong. Aggressive Behavior Comparisons of TibetanMacaques from Mt. Huangshan, China (Macaca thibetana) According to Their Age and Sex[J]. ZOOLOGICAL RESEARCH, 2010, 31(4): 428-434.
[15] CHEN Jun,LI Qi,KONG Ling-Feng,ZHENG Xiao-Dong,YU Rui-Hai. COI-based DNA Barcoding in Tapetinae Species (Mollusca, Bivalvia, Veneridae) along the Coast of CHINA[J]. ZOOLOGICAL RESEARCH, 2010, 31(4): 345-352.
Full text