ZOOLOGICAL RESEARCH 2012, 33(1) 85-88 DOI:   10.3724/SP.J.1141.2012.01085   ISSN: 0254-5853 CN: 53-1040/Q

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Keywords
Rhesus monkey
Neural progenitor cells
Vitreous
Transplant
Authors
PubMed

A technique of rhesus monkey neural progenitor cells intravitreal transplant to rats

BIAN Hui1,2, FAN Yao-Dong1,3, GUO Li-Yun4, YU Hua-Lin1

1. Department of Minimally Invasive Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032, China;
2. Department of Physiology, Kunming Medical College, Kunming Yunnan 650031, China;
3. Department of Neurosurgery, Third Affiliated Hospital of Kunming Medical College, Kunming Yunnan 650106, China;
4. Department of Ophthalmology, Fourth Affiliated Hospital of Kunming Medical College, Kunming 650021, China

Abstract

To investigate a simple and effective intraocular xenotransplant technique of rhesus monkey neural progenitor cells to rats, mechanical injury was induced in the rat’s right retina. And the GFP-labeled rhesus monkey neural progenitor cells suspension was slowly injected into the vitreous space of the right injured and left control eye. Confocal image suggested that the xenografted cells survived in both the injured and control eye, meanwhile the cells integrated in the injured right retina. The results demonstrated that intravitreal xenotransplant could be adopted as a simple and reliable method.

Keywords Rhesus monkey   Neural progenitor cells   Vitreous   Transplant  
Received 2011-10-27 Revised 2011-12-30 Online:  
DOI: 10.3724/SP.J.1141.2012.01085
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Email: yuhl308@126.com
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