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ZOOLOGICAL RESEARCH    2012, Vol. 33 Issue (1) : 85-88     DOI: 10.3724/SP.J.1141.2012.01085
Reports |
A technique of rhesus monkey neural progenitor cells intravitreal transplant to rats
BIAN Hui1,2, FAN Yao-Dong1,3, GUO Li-Yun4, YU Hua-Lin1
1. Department of Minimally Invasive Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032, China;
2. Department of Physiology, Kunming Medical College, Kunming Yunnan 650031, China;
3. Department of Neurosurgery, Third Affiliated Hospital of Kunming Medical College, Kunming Yunnan 650106, China;
4. Department of Ophthalmology, Fourth Affiliated Hospital of Kunming Medical College, Kunming 650021, China
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Abstract  

To investigate a simple and effective intraocular xenotransplant technique of rhesus monkey neural progenitor cells to rats, mechanical injury was induced in the rat’s right retina. And the GFP-labeled rhesus monkey neural progenitor cells suspension was slowly injected into the vitreous space of the right injured and left control eye. Confocal image suggested that the xenografted cells survived in both the injured and control eye, meanwhile the cells integrated in the injured right retina. The results demonstrated that intravitreal xenotransplant could be adopted as a simple and reliable method.

Keywords Rhesus monkey      Neural progenitor cells      Vitreous      Transplant     
PACS:  Q959.848  
  R779.65  
  Q813.3  
Issue Date: 22 February 2012
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BIAN Hui, FAN Yao-Dong, GUO Li-Yun, YU Hua-Lin. A technique of rhesus monkey neural progenitor cells intravitreal transplant to rats[J]. ZOOLOGICAL RESEARCH,2012, 33(1): 85-88.
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http://www.zoores.ac.cn/EN/10.3724/SP.J.1141.2012.01085     OR     http://www.zoores.ac.cn/EN/Y2012/V33/I1/85

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