ZOOLOGICAL RESEARCH 2012, 33(1) 19-28 DOI:   10.3724/SP.J.1141.2012.01019   ISSN: 0254-5853 CN: 53-1040/Q

Current Issue | Archive | Search                                                            [Print]   [Close]
Information and Service
This Article
Supporting info
Service and feedback
Email this article to a colleague
Add to Bookshelf
Add to Citation Manager
Cite This Article
Email Alert
Tree shrew
Sperm cryopreservation

Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri)

PING Shu-Huang, WANG Cai-Yun, TANG Wen-Ru, LUO Ying, YANG Shi-Hua

Laboratory of Molecular Genetics of Aging and Tumor, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China


The tree shrew may be an important experimental animal for disease models in humans. The effects of some extenders and momamines on sperm cryopreservation will provide helpful data for experimentation of strains and conservation of genetic resources in tree shrews. Epididymal sperm were surgically harvested from male tree shrews captured around Kunming, China and sperm motility, acrosome integrity and fertility were assessed during cryopreservation. In Experiment 1 eight extenders (TTE, TCG, TCF, TTG, BWW, BTS, DM, and SR) supplemented with 0.4 mol/L DMSO were used to dilute the sperm: only TTE, DM and SR showed no differences in motility and acrosome integrity compared to fresh controls after equilibration. After freezing and thawing, sperm in any extender showed lower motility than fresh control and sperm in DM showed higher motility than other groups. However, BWW produced the lowest motility. For acrosome intergrity, TTE and DM showed higher than BWW, BTS and SR after equilibration. The parameter in DM was higher than other groups (except TTE) after thawing. In Experiment 2 four penetrating cryoprotectant agents (CPA) [dimethyl-formamide (DF), formamide (F), dimethylacetamide (DA), and acetamide (A)] at 0.2 mol/L, 0.4 mol/L, 0.8 mol/L, and 1.2 mol/L, respectively were added to the DM extender. Motility showed no difference among CPA groups and non-CPA group (control) after equilibration, but all thawed sperm showed lower values in motility and acrosome intergrity than pre-freezing groups. However, sperm in 0.8 mol/L DF and 0.4 mol/L DMSO showed higher values in both parameters than that in other CPA groups (P>0.05). In Experiment 3 the fertilization rate of oocytes inseminated with 0.4mol/L DMSO (50%) were higher than that with 0.8mol/L DF (16%). In conclusion, non-ion extenders supplemented with egg yolk may be better for sperm cryopreservation in tree shrews and cryoprotectant effects of monoamines agents should be further studied in this species.

Keywords Tree shrew   Sperm cryopreservation   Extender   Monoamines  
Received 2011-11-01 Revised 2011-12-28 Online:  
DOI: 10.3724/SP.J.1141.2012.01019
Corresponding Authors:
Email: huashiyang@hotmail.com
About author:

Similar articles
1.XU Lin1,2, ZHANG Yun1, LIANG Bin1, Lü Long-Bao2,3, CHEN Ce-Shi1, CHEN Yong-Bin1,.Tree shrews under the spot light: emerging model of human diseases[J]. ZOOLOGICAL RESEARCH, 0,(): 203-
2.XU Ling 1,3, FAN Yu 1,3, JIANG Xue-Long 2, YAO Yong-Gang 1,*.Molecular evidence on the phylogenetic position of tree shrews[J]. ZOOLOGICAL RESEARCH, 0,(): 309-
3.Xiaohong LIU, Yonggang YAO.Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis)[J]. ZOOLOGICAL RESEARCH, 0,(): 60-
4.CAO Xiao-mei.Seasonal Changes in Spermatogenesis of Tree Shrew (Tupaia Belangeri Chinensis)[J]. ZOOLOGICAL RESEARCH, 1989,10(1): 15-21
5.YE Zhi-zhang;PENG Yan-zhang;PAN Ru-liang;WANG Hong.The spinal plexus of Tree shrew(Tupaia belangeri chinensis)[J]. ZOOLOGICAL RESEARCH, 1989,10(2): 107-114
6.LIU Rui-qing; SHI Li-ming; CHEN Yu-ze.Comparative Studies on Chromosoes of 3 Subspecies of Tupaia belangeri[J]. ZOOLOGICAL RESEARCH, 1989,10(3): 195-200
7.ZOU Ru-jin;TIAN Bao-pin;JI Wei-zhi;LI Rui-yuan.Research on The Piebaldism of Tree Shrews Breeding in The Captivity[J]. ZOOLOGICAL RESEARCH, 1989,10(zk): 68-78
8.LU Jin-ming;ZOU Ru-jin;JI Wei-zhi;XIE Yun-hua;YAN Ye WEI Yun-zhen.The Feed Contained Different Proteints Influences the Nitrogen Balance in Tree Shrews[J]. ZOOLOGICAL RESEARCH, 1989,10(zk): 111-117
9.ZHANG Ya-ping;ZHANG Bing;SHI Li-ming.Restriction Maps of Mitochondrial DNA of Slow Loris and Tree Shrew[J]. ZOOLOGICAL RESEARCH, 1989,10(zk): 79-89
10.TIAN Bao-ping;PEN Yian-zhang;HOU Yi-di.Investigation of Parasites on Chinese Tree Shrews[J]. ZOOLOGICAL RESEARCH, 1989,10(zk): 90-110
11.MA Cai-xia;MA Kun;SHI Li-ming.Electron Microscopic Observations on The Synaptonemal Complex in Spermatocytes of The Tree Shrew (Tupaiabelangeri Chinensis)[J]. ZOOLOGICAL RESEARCH, 1989,10(zk): 29-33
12.JI Wei-zhi;ZOU Ru-jin;YANG Shang-chuan.A Comparative Study of Lactate Dehydrogenase (LDH) Isozymes in Yunnan and Guangxi Tree Shrews[J]. ZOOLOGICAL RESEARCH, 1989,10(zk): 69-77
13.CAO Xiao-mei.Microscopic Structure of Ovary and Ovarian Activity of Different Seasons in Tree Shrew (Tupaia belangeri chinensis)[J]. ZOOLOGICAL RESEARCH, 1990,11(1): 17-24
14.ZHANG Qing-zhen;YE Zheng;SHEN Ban-kun;LI Lang.The Willis Circle in Tupaia belangeri chinensis[J]. ZOOLOGICAL RESEARCH, 1990,11(1): 41-45

Comment for this article: