Please wait a minute...
ZOOLOGICAL RESEARCH    2012, Vol. 33 Issue (1) : 19-28     DOI: 10.3724/SP.J.1141.2012.01019
Articles |
Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri)
PING Shu-Huang, WANG Cai-Yun, TANG Wen-Ru, LUO Ying, YANG Shi-Hua
Laboratory of Molecular Genetics of Aging and Tumor, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
Download: PDF(536 KB)  
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks    
Abstract  

The tree shrew may be an important experimental animal for disease models in humans. The effects of some extenders and momamines on sperm cryopreservation will provide helpful data for experimentation of strains and conservation of genetic resources in tree shrews. Epididymal sperm were surgically harvested from male tree shrews captured around Kunming, China and sperm motility, acrosome integrity and fertility were assessed during cryopreservation. In Experiment 1 eight extenders (TTE, TCG, TCF, TTG, BWW, BTS, DM, and SR) supplemented with 0.4 mol/L DMSO were used to dilute the sperm: only TTE, DM and SR showed no differences in motility and acrosome integrity compared to fresh controls after equilibration. After freezing and thawing, sperm in any extender showed lower motility than fresh control and sperm in DM showed higher motility than other groups. However, BWW produced the lowest motility. For acrosome intergrity, TTE and DM showed higher than BWW, BTS and SR after equilibration. The parameter in DM was higher than other groups (except TTE) after thawing. In Experiment 2 four penetrating cryoprotectant agents (CPA) [dimethyl-formamide (DF), formamide (F), dimethylacetamide (DA), and acetamide (A)] at 0.2 mol/L, 0.4 mol/L, 0.8 mol/L, and 1.2 mol/L, respectively were added to the DM extender. Motility showed no difference among CPA groups and non-CPA group (control) after equilibration, but all thawed sperm showed lower values in motility and acrosome intergrity than pre-freezing groups. However, sperm in 0.8 mol/L DF and 0.4 mol/L DMSO showed higher values in both parameters than that in other CPA groups (P>0.05). In Experiment 3 the fertilization rate of oocytes inseminated with 0.4mol/L DMSO (50%) were higher than that with 0.8mol/L DF (16%). In conclusion, non-ion extenders supplemented with egg yolk may be better for sperm cryopreservation in tree shrews and cryoprotectant effects of monoamines agents should be further studied in this species.

Keywords Tree shrew      Sperm cryopreservation      Extender      Monoamines     
PACS:  Q954.43  
  Q959.832  
  Q492  
Issue Date: 22 February 2012
Service
E-mail this article
E-mail Alert
RSS
Articles by authors
Cite this article:   
PING Shu-Huang, WANG Cai-Yun, TANG Wen-Ru, LUO Ying, YANG Shi-Hua. Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri)[J]. ZOOLOGICAL RESEARCH,2012, 33(1): 19-28.
URL:  
http://www.zoores.ac.cn/EN/10.3724/SP.J.1141.2012.01019     OR     http://www.zoores.ac.cn/EN/Y2012/V33/I1/19

Anel L, Kaabi M, Abroug B, Alvarez M, Anel E, Boixo JC, de la Fuente LF, de Paz P. 2005. Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay
[J]. Theriogenology, 63(4): 1235-1247.
Bathgate R, Maxwell WM, Evans G. 2006. Studies on the effect of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality
[J]. Reprod Domest Anim, 41(1): 68-73.
Bavister BD, Leibfried ML, Lieberman G. 1983. Development of preimplantation embryos of the golden hamster in a defined culture medium
[J]. Biol Reprod, 28(1): 235-247.
Bianchi I, Calderam K, Maschio EF, Madeira EM, da Rosa Ulguim R, Corcini CD, Bongalhardo DC, Correa EK, Lucia T Jr, Deschamps JC, Correa MN. 2008. Evaluation of amides and centrifugation temperature in boar semen cryopreservation
[J]. Theriogenology, 69(5): 632-638.
Cao XM, Ben KL, Wang XL. 2001. Ovulation in the tree shrew (Tupaia belangeri) induced by gonadotrophins
[J]. Reprod Fertil Dev, 13(5-6): 377-382.
Chen S, Xu L, Lü LB, Yao YG. 2011. Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China
[J]. Zool Res (in Chinese), 32(1): 17-23.
Collins PM, Tsang WN, Urbanski HF. 2007. Endocrine correlates of reproductive development in the male tree-shrew (Tupaia belangeri) and the effects of infantile exposure to exogenous androgens
[J]. Gen Comp Endocrinol 154(1-3): 22-30.
Coscioni AC, Reichenbach HD, Schwartz J, LaFalci VS, Rodrigues JL, Brandelli A. 2001. Sperm function and production of bovine embryos in vitro after swim-up with different calcium and caffeine concentration
[J]. Anim Reprod Sci, 67(1-2): 59-67.
Coulter GH, Foote RH. 1975. Lipid deficient extender for bovine spermatozoa: its development and use in measuring freezing-induced lipid loss
[J]. J Dairy Sci 58(1): 82-87.
De Graaf SP, Evans G, Maxwell WM, Cran DG, O'Brien JK. 2007. Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa
[J]. Theriogenology, 67(2): 391-398.
Dube C, Beaulieu M, Reyes-Moreno C, Guillemette C, Bailey JL. 2004. Boar sperm storage capacity of BTS and Androhep Plus: viability, motility, capacitation, and tyrosine phosphorylation
[J]. Theriogenology, 62(5): 874-886.
Farshad A, Khalili B, Fazeli P. 2009. The effect of different concentrations of glycerol and DMSO on viability of Markhoz goat spermatozoa during different freezing temperatures steps
[J]. Pak J Biol Sci, 12(3): 239-245.
Fernandez-Santos MR, Esteso MC, Montoro V, Soler AJ, Garde JJ. 2006. Influence of various permeating cryoprotectants on freezability of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa: effects of concentration and temperature of addition
[J]. J Androl, 27(6): 734-745.
Fernandez-Santos MR, Esteso MC, Soler AJ, Montoro V, Garde JJ. 2005. The effects of different cryoprotectants and the temperature of addition on the survival of red deer epididymal spermatozoa
[J]. Cryo Letters, 26(1): 25-32.
Futino DO, Mendes MC, Matos WN, Mondadori RG, Lucci CM. 2010. Glycerol, methyl-formamide and dimethyl-formamide in canine semen cryopreservation
[J]. Reprod Domest Anim, 45(2): 214-220.
Gabriel Sanchez-Partida L, Maginnis G, Dominko T, Martinovich C, McVay B, Fanton J, Schatten G. 2000. Live rhesus offspring by artificial insemination using fresh sperm and cryopreserved sperm
[J]. Biol Reprod, 63(4): 1092-1097.
Garde JJ, del Olmo A, SolerAJ, Espeso G, Gomendio M, Roldan ER. 2008. Effect of egg yolk, cryoprotectant, and various sugars on semen cryopreservation in endangered Cuvier's gazelle (Gazella cuvieri)
[J]. Anim Reprod Sci, 108(3-4): 384-401.
Juliani GC, Henry M. 2008. Effects of glycerol, ethylene glycol, acetamide, and dried skim milk in cryopreservation of equine sperm
[J]. Arq Bras Med Vet Zoo, 60(5): 1103-1109.
Kaneko T, Yamamura A, Ide Y, Ogi M, Yanagita T, Nakagata N. 2006. Long-term cryopreservation of mouse sperm
[J]. Theriogenology, 66(5):1098-1101.
Kashiwazaki N, Okuda Y, Seita Y, Hisamatsu S, Sonoki S, Shino M, Masaoka T, Inomata T. 2006. Comparison of glycerol, lactamide, acetamide and dimethylsulfoxide as cryoprotectants of Japanese white rabbit spermatozoa
[J]. J Reprod Dev, 52(4): 511-516.
Kozicz T, Bordewin LA, Czeh B, Fuchs E, Roubos EW. 2008. Chronic psychosocial stress affects corticotropin-releasing factor in the paraventricular nucleus and central extended amygdala as well as urocortin 1 in the non-preganglionic Edinger-Westphal nucleus of the tree shrew
[J]. Psychoneuroendocrinology, 33(6): 741-754.
Li Y, Wan DF, Wei W, Su JJ, Cao J, Qiu XK, Ou C, Ban KC, Yang C, Yue HF. 2008. Candidate genes responsible for human hepatocellular carcinoma identified from differentially expressed genes in hepatocarcinogenesis of the tree shrew (Tupaia belangeri chinesis)
[J]. HepatolRes,38(1): 85-95.
Li YH, Cai KJ, Kovacs A, Ji WZ. 2005. Effects of various extenders and permeating cryoprotectants on cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa
[J]. J Androl,26(3): 387-395.
Li YH, Cai KJ, Li J, Dinnyes A, Ji WZ. 2006. Comparative studies with six extenders for sperm cryopreservation in the cynomolgus monkey (Macaca fascicularis) and rhesus monkey (Macaca mulatta)
[J]. Am J Primatol, 68(1): 39-49.
Li YH, Cai KJ, Su L, Guan M He XC, Wang H, Kovacs A, Ji WZ 2005. Cryopreservation of cynomolgus monkey (Macaca fascicularis) spermatozoa in a chemically defined extender
[J]. Asian J Androl, 7(2): 139-144.
Martins SG, Miranda PV, Brandelli A. 2003. Acrosome reaction inhibitor released during in vitro sperm capacitation
[J]. Int J Androl, 26(5): 296-304.
Moce E, Vicente JS, Lavara R. 2003. Effect of freezing-thawing protocols on the performance of semen from three rabbit lines after artificial insemination
[J]. Theriogenology, 60(1): 115-123.
Moustacas V, Cruz B, Varago F, Miranda D, Lage P, Henry M. 2011. Extenders containing dimethylformamide associated or not with glycerol are ineffective for ovine sperm cryopreservation
[J]. Reprod Domest Anim, 46(5): 924-925
O'Meara CM, Hanrahan JP, Donovan A, Fair S, Rizos D, Wade M, Boland MP, Evans AC, P Lonergan. 2005. Relationship between in vitro fertilisation of ewe oocytes and the fertility of ewes following cervical artificial insemination with frozen-thawed ram semen
[J]. Theriogenology, 64(8): 1797-1808.
Paudel KP, Kumar S, Meu SK, Kumaresan A. 2010. Ascorbic acid, catalase and chlorpromazine reduce cryopreservation-induced damages to crossbred bull spermatozoaa
[J]. Reprod Domest Anim, 45(2): 256-262.
Ping S, Wang F, Zhang Y, Wu C, Tang W, Luo Y, Yang S. 2011. Cryopreservation of epididymal sperm in tree shrews (Tupaia belangeri)
[J]. Theriogenology, 76(1): 39-46.
Poonkhum R, Pongmayteegul S, Meeratana W, Pradidarcheep W, Thongpila S, Mingsakul T, Somana R. 2000. Cerebral microvascular architecture in the common tree shrew (Tupaia glis) revealed by plastic corrosion casts
[J]. Microsc Res Tech, 50(5): 411-418.
Poveda A, Kretz R. 2009. c-Fos expression in the visual system of the tree shrew (Tupaia belangeri)
[J]. J Chem Neuroanat, 37(4): 214-228.
Rasul Z, Ahmad N, Anzar M. 2001. Changes in motion characteristics, plasma membrane integrity, and acrosome morphology during cryopreservation of buffalo spermatozoa
[J]. J Androl, 22(2): 278-283.
Remple MS, Reed JL, Stepniewska I, Lyon DC, Kaas JH. 2007. The organization of frontoparietal cortex in the tree shrew (Tupaia belangeri): II. Connectional evidence for a frontal-posterior parietal network
[J]. J Comp Neurol, 501(1): 121-149.
Santiago-Moreno J, Coloma MA, Dorado J, Pulido-Pastor A, Gomez-Guillamon F, Salas-Vega R, Gomez-Brunet A, Lopez-Sebastian A. 2009. Cryopreservation of Spanish ibex (Capra pyrenaica) sperm obtained by electroejaculation outside the rutting season
[J]. Theriogenology, 71(8): 1253-1260.
Santiago-Moreno J, Coloma MA, Toledano-Diaz A, Gomez-Brunet A, Pulido-Pastor A, Zamora-Soria A, Carrizosa JA, Urrutia B, Lopez-Sebastian A. 2008. A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa
[J]. Cryobiology, 57(1): 25-29.
Santiago-Moreno J, Toledano-Diaz A, Pulido-Pastor A, Dorado J, Gomez-Brunet A, Lopez-Sebastian A. 2006. Effect of egg yolk concentration on cryopreserving Spanish ibex (Capra pyrenaica) epididymal spermatozoa
[J]. Theriogenology, 66(5): 1219-1226.
Saragusty J, Gacitua H, Rozenboim I, Arav A. 2009. Protective effects of iodixanol during bovine sperm cryopreservation
[J]. Theriogenology, 71(9): 1425-1432.
Si W, Zheng P, Li YH, Dinnyes A, Ji WZ. 2004. Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm
[J]. Am J Primatol, 62(4): 301-306.
Si W, Zheng P, Tang X, He X, Wang H, Bavister BD, Ji W. 2000. Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization
[J]. Cryobiology, 41(3): 232-240.
Sztein JM, Farley JS, Mobraaten LE. 2000. In vitro fertilization with cryopreserved inbred mouse sperm
[J]. Biol Reprod, 63(6): 1774-1780.
Tateno H, Mikamo K. 1987. A chromosomal method to distinguish between X- and Y-bearing spermatozoa of the bull in zona-free hamster ova
[J]. J Reprod Fertil, 81(1): 119-125.
Underwood SL, Bathgate R, Maxwell WM, Evans G. 2010. Birth of offspring after artificial insemination of heifers with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm
[J]. Anim Reprod Sci, 118(2-4): 171-175.
Xu XP, Chen HB, Cao XM, Ben KL. 2007. Efficient infection of tree shrew (Tupaia belangeri) with hepatitis C virus grown in cell culture or from patient plasma
[J]. J Gen Virol 88: 2504-2512.
Yeste M, Briz M, Pinart E, Sancho S, Garcia-Gil N, Badia E, Bassols J, Pruneda A, Bussalleu E, Casas I, Bonet S.2008. Hyaluronic acid delays boar sperm capacitation after 3 days of storage at 15 degrees C
[J]. Anim Reprod Sci, 109(1-4): 236-250.
Yildiz C, Kaya A, Aksoy M, Tekeli T. 2000. Influence of sugar supplementation of the extender on motility, viability and acrosomal integrity of dog spermatozoa during freezing
[J]. Theriogenology,54(4): 579-585.
Zambello E, Fuchs E, Abumaria N, Rygula R, Domenici E, Caberlotto L. 2010. Chronic psychosocial stress alters NPY system: different effects in rat and tree shrew
[J]. Prog Neuropsychopharmacol Biol Psychiatry, 34(1): 122-130.

[1] Yong-Gang Yao. Creating animal models, why not use the Chinese tree shrew (Tupaia belangeri chinensis)?[J]. Zoological Research, 2017, 38(3): 118-126.
[2] Ji Xiao, Rong Liu, Ce-Shi Chen. Tree shrew (Tupaia belangeri) as a novel non-human primate laboratory disease animal model[J]. ZOOLOGICAL RESEARCH, 2017, 38(3): 127-137.
[3] Shu Wei, Hai-Rong Hua, Qian-Quan Chen, Ying Zhang, Fei Chen, Shu-Qing Li, Fan Li, Jia-Li Li. Dynamic changes in DNA demethylation in the tree shrew (Tupaia belangeri chinensis) brain during postnatal development and aging[J]. ZOOLOGICAL RESEARCH, 2017, 38(2): 96-102.
[4] Lan-Zhen YAN, Bin SUN, Long-Bao LYU, Yu-Hua MA, Jia-Qi CHEN, Qing LIN, Ping ZHENG, Xu-Dong ZHAO. Early embryonic development and transplantation in tree shrews[J]. ZOOLOGICAL RESEARCH, 2016, 37(4): 252-258.
[5] Jian-Ping LI, Yun LIAO, Ying ZHANG, Jing-Jing WANG, Li-Chun WANG, Kai FENG, Qi-Han LI, Long-Ding LIU. Experimental infection of tree shrews(Tupaia belangeri) with Coxsackie virus A16[J]. ZOOLOGICAL RESEARCH, 2014, 35(6): 485-491.
[6] Gui LI, Ren LAI, Gang DUAN, Long-Bao LYU, Zhi-Ye ZHANG, Huang LIU, Xun XIANG. Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews[J]. ZOOLOGICAL RESEARCH, 2014, 35(6): 492-499.
[7] Yu FAN, Dan-Dan YU, Yong-Gang YAO. Positively selected genes of the Chinese tree shrew (Tupaia belangeri chinensis) locomotion system[J]. ZOOLOGICAL RESEARCH, 2014, 35(3): 240-248.
[8] Jing WANG, Xin-Li XU, Ze-Yang DING, Rong-Rong MAO, Qi-Xin ZHOU, Long-Bao LÜ, Li-Ping WANG, Shuang WANG, Chen ZHANG, Lin XU, Yue-Xiong YANG. Basal physiological parameters in domesticated tree shrews (Tupaia belangeri chinensis)[J]. ZOOLOGICAL RESEARCH, 2013, 34(E2): 13369-E.
[9] Xiao-Hong LIU, Yong-Gang YAO. Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis)[J]. ZOOLOGICAL RESEARCH, 2013, 34(E2): 13362-E.
[10] Xiao-Yun WU, Yun-Hai LI, Qing CHANG, Lin-Qiang ZHANG, Sha-Sha LIAO, Bin LIANG. Streptozotocin induction of type 2 diabetes in tree shrew[J]. ZOOLOGICAL RESEARCH, 2013, 34(2): 108-115.
[11] Ling XU, Yu FAN, Xue-Long JIANG, Yong-Gang YAO. Molecular evidence on the phylogenetic position of tree shrews[J]. ZOOLOGICAL RESEARCH, 2013, 34(2): 70-76.
[12] Lin XU, Yun ZHANG, Bin LIANG, Long-Bao LÜ, Ce-Shi CHEN, Yong-Bin CHEN, Ju-Min ZHOU, Yong-Gang YAO. Tree shrews under the spot light: emerging model of human diseases[J]. ZOOLOGICAL RESEARCH, 2013, 34(2): 59-69.
[13] Bo LI, Rong-Ping ZHANG, Jin-Tao LI, Bao-Li HE, Hong ZHEN, Li-Mei WANG, Jian-Lin JIAO. Measurement and analysis of anatomical parameter values in tree shrews[J]. ZOOLOGICAL RESEARCH, 2013, 34(2): 132-138.
[14] Cai-Yun WANG, Yun-Han MA, Da-Jian HE, Shi-Hua YANG. cDNA cloning and sequence analysis of pluripotency genes in tree shrews (Tupaia belangeri)[J]. ZOOLOGICAL RESEARCH, 2013, 34(2): 127-131.
[15] Xiao-Yan HUANG, Ming-Li LI, Juan XU, Yue-Dong GAO, Wen-Guang WANG, An-Guo YIN, Xiao-Fei LI, Xiao-Mei SUN, Xue-Shan XIA, Jie-Jie DAI. Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews[J]. ZOOLOGICAL RESEARCH, 2013, 34(2): 121-126.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed