ZOOLOGICAL RESEARCH 2011, 32(4) 363-370 DOI:   10.3724/SP.J.1141.2011.04363   ISSN: 0254-5853 CN: 53-1040/Q

Current Issue | Archive | Search                                                            [Print]   [Close]
Information and Service
This Article
Supporting info
Service and feedback
Email this article to a colleague
Add to Bookshelf
Add to Citation Manager
Cite This Article
Email Alert
Qiantang River
Macrobrachium nipponense
Genetic diversity
Genetic structure

Microsatellite analysis of genetic variation of the oriental river prawn Macrobrachium nipponense in Qiantang River

MA Ke-Yi1, FENG Jian-Bin1, XIE Nan2, FENG Xiao-Yu2, LI Jia-Le1,*

1.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China; 2. Fishery Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China


Genetic diversity and genetic structure of 7 wild stocks of oriental river prawn Macrobrachium nipponense in Qiantang River, i.e. Wen-yan, Fu-yang, Chang-kou, Tong-lu, Xin-an-jiang, She-xian and Xiu-ning, were investigated using 10 microsatellite DNA markers. The result showed that all the 10 loci were highly polymorphic. There was a trend that the level of genetic diversity of wild stocks in downstream and midstream were higher than the upstream ones’. Sign test and Wilcoxon sign rank test results showed that the stocks in Qiantang River had no bottleneck effect, and the number of stocks had not declined recently. FST among stocks ranged from 0.0201 to 0.1069. Analysis of molecular variance (AMOVA) revealed that a higher portion (93.48%) of variations existed within individuals, while lower portion (6.52%) existed among stocks. FST and AMOVA analysis across all stocks and loci indicated the medium level of divergence among the stocks. The NJ clustering tree based on DA genetic distance demonstrated that the stocks of adjacent geographical position clustered together. 413 individuals obtained from six wild stocks could be divided into two potential populations based on the genetic structure. This study demonstrated that genetic diversity and genetic structure of M. nipponense stocks were relevant to geographical position where they survived.

Keywords Qiantang River   Macrobrachium nipponense   Microsatellite   Genetic diversity   Genetic structure  
Received 2010-11-08 Revised 2011-05-02 Online: 2011-08-22 
DOI: 10.3724/SP.J.1141.2011.04363
Corresponding Authors: LI Jia-Le
Email: E-mail: jlli2009@126.com
About author: MA Ke-Yi,E-mail: mky850125@163.com


 Botstein D, White RL, Skolnick M, Davis RW. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms [J]. Am J Hum Genet, 32: 31-34.
 Brooker AL, Benzie JAH, Blair D, Versini JJ. 2000. Population structure of the giant tiger prawn, Penaeus monodon, in Australian waters using microsatellite markers [J]. Mar Biol, 136: 149-157.
 Brown AHD, Clegg MT. 1989. Plant Population Genetics, Breeding, and Genetic Resources [M]. Sunderland, Massachusetts: Sinauer Associates, 43-63.
 Chand V, de Bruyn M, Mather PB. 2005. Microsatellite loci in the eastern form of the giant freshwater prawn (Macrobrachium rosenbergii) [J]. Mol Ecol Notes, 5: 308-310.
 Cornuet JM, Luikart G. 1997. Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data [J]. Genetics, 144: 2001-2014.
 Evanno G, Regnaut S, Goudet J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study [J]. Mol Ecol, 14: 2611-2620.
 Excoffier L, Laval G, Schneider S. 2005. Arlequin ver. 3.0: An integrated software package for population genetics data analysis [J]. Evol Bioinform Online, 1: 47-50.
 Falush D, Stephens M, Pritchard JK. 2003. Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies [J]. Genetics, 164: 1567-1587.
 Feng JB, Li JL, Cheng X. 2008. Research progress on germplasm resource exploitation and protection of Macrobrachium nipponense [J]. J Shanghai Fish Univ, 15(3): 371-376. (in Chinese) [冯建彬, 李家乐, 程熙. 2008. 日本沼虾种质资源挖掘和保护研究进展. 上海水产大学学报, 15(3): 371-376.]
 Feng JB, Ma KY, Li JL, Hua XM, Ding HY. 2010. Isolation and genetic diversity analysis of microsatellite DNA in Macrobrachium nipponense [J]. J Fish Chn, 34(5): 688-695. (in Chinese) [冯建彬, 马克异, 李家乐, 华雪铭, 丁怀宇. 2010. 日本沼虾微卫星引物筛选及群体遗传多样性分析. 水产学报, 34(5): 688-695.]
 Feng JB, Sun YN, Cheng X, Li JL. 2008. Sequence analysis of mitochondrial COI gene of Macrobrachium nipponense from the five largest freshwater lakes in China
[J]. J Fish Chn, 32(4): 517-525. (in Chinese) [冯建彬, 孙悦娜, 程熙, 李家乐. 2008. 我国五大湖青虾线粒体COI基因片段序列比较研究. 水产学报, 32(4): 517-525.]
 Feng JB, Wu CL, Ding HY, Hua XM, Li YS, Li JL. 2010. Microsatellite analysis of genetic diversity in 9 wild stocks of oriental river prawn Macrobrachium nipponense in Hongze Lake [J]. J Fish Sci Chn, 17(2): 1-10. (in Chinese) [冯建彬, 吴春林, 丁怀宇, 华雪铭, 李应森, 李家乐. 洪泽湖日本沼虾9个野生群体遗传多样性微卫星分析. 中国水产科学, 17(2): 1-10.]
 Fu HT, Qiao H, Li FJ, Wu Y, Gong YS, Jiang SF, Xiong YW, Wang N. 2010. Genetic diversity of Macrobrachium nipponense on a regional scale in the Yangtze River [J]. J Fish Chn, 34(2): 204-212. (in Chinese) [傅洪拓, 乔慧, 李法君, 吴滟, 龚永生, 蒋速飞, 熊贻伟, 王宁. 2010. 长江不同江段青虾的遗传多样性. 水产学报, 34(2): 204-212.]
 Hartl DL, Clark AG.. 1997. Principles of Population Genetics [M]. 3nd ed. Sunderland, MA: Sinauer Associates, Inc., 74-110.
 Jiang SF, Fu HT, Xiong YW, Wu Y, Gong YS, He XL. 2006. Genetic diversity of four geographical populations of Macrobrachium nipponense revealed by RAPD analysis
[J]. J Yangtze Univ: Natural Science Edition, 3(2): 179-182. (in Chinese)
[蒋速飞 傅洪拓 熊贻伟 吴滟 龚永生 何新龙. 2006. 日本沼虾4个地理群体遗传变异的RAPD分析. 长江大学学报:自然科学版, 3(2): 179-182.]
 Kancee C, Supawadee P, Uthairat N, Wongpathom K. 2007. Genetic diversity of hatchery stocks of giant freshwater prawn (Macrobrachium rosenbergii) in Thailand
[J]. Aquaculture, 271: 121-129.
 Li JL, Nie SZ, Feng JB, He W, Fan YP, Cheng X. 2005. The comparison of growth and culture performance in cage for five stocks of Oriental river prawn from the midstream and downstream of Yangtze River [J]. J Shanghai Fish Univ, 14(3): 258-262. (in Chinese) [李家乐, 聂式忠, 冯建彬, 何 为,范益平, 程熙. 2005. 长江中下游五个青虾群体网箱生长和养殖性能比较. 上海水产大学学报, 14(3): 258-262.]
 Maruyama T, Fuerst PA. 1985. Population bottlenecks and nonequilibrium models in population genetics. Number of alleles in a small population that was formed by a recent bottleneck [J]. Genetics, 111: 675-689.
 Nei M, Jajima F, Tateno Y. 1983. Accuracy of estimated phylogenetic trees from molecular data [J]. J Mol Evol, 19: 153-170.
 Rousset F. 2008. GENEPOP’007: a complete e-implementation of the GENEPOP software for Windows and Linux [J]. Mol Ecol Resour, 8:103-106.
 Sambrook J, Russell DW. 2001. Molecular Cloning: A Laboratory Manual, 3rd edition [M]. NY: Cold Spring Harbor Laboratory Press.
 Sun YN, Feng JB, Li JL, Nie SZ. 2007. Sequence analysis and molecular phylogeny of mitochondrial 16S rRNA gene fragments in three populations of Macrobrachium nipponense [J]. Chn J Zool, 42(1): 59-66. (in Chinese)
[孙悦娜, 冯建彬, 李家乐, 聂式忠. 2007. 日本沼虾三群体线粒体16S rRNA基因片段序列的差异与系统进化. 动物学杂志, 42(1): 59-66.]
 Wu Y, Fu HT, Li JL, Gong YS, Li MS. 2008. Research on genetic diversity of Macrobrachium nipponense in Taihu Lake [J]. J Shanghai FishUniv, 17(5): 620-624. (in Chinese)
[吴滟, 傅洪拓, 李家乐, 龚永生, 李明爽. 2008. 太湖日本沼虾的遗传多样性分析. 上海水产大学学报, 17(5): 620-624.]
 Yang P, Zhang H, Chen LQ, Ye JY, Yu N, Gu ZM, Song DX. 2007. Genetic structure of the Oriental river prawn (Macrobrachium nipponense) from the Yangtze and Lancang Rivers, inferred from COI gene sequence [J]. Zool Res, 28(2): 113-118. (in Chinese) [杨频, 张浩, 陈立侨, 叶金云, 禹娜, 顾志敏, 宋大祥. 2007. 利用COI基因序列分析长江与澜沧江水系日本沼虾群体的遗传结构. 动物学研究, 28(2): 113-118.]
 Zhu YA, Shan H, Wang Q, Zhou GQ. 2008. Genetic diversity of three geographical populations of Macrobrachium nipponense revealed by RAPD analysis [J]. J Aquac, 29(1): 5-7. (in Chinese) [朱银安, 单红, 王庆, 周国勤. 2008. 长江、高邮湖、太湖日本沼虾遗传多样性的RAPD分析. 水产养殖, 29(1): 5-7.]


Similar articles
1.MA Ke-Yi, FENG Jian-Bin, XIE Nan, FENG Xiao-Yu, LI Jia-Le,*.Microsatellite analysis of genetic variation of the oriental river prawn Macrobrachium nipponense in Qiantang River[J]. ZOOLOGICAL RESEARCH, 2011,3(4): 363-370

Comment for this article: