Please wait a minute...
ZOOLOGICAL RESEARCH    2011, Vol. 32 Issue (3) : 323-328     DOI: 10.3724/SP.J.1141.2011.03323
Articles |
Acute effects of IL-1β on sodium current in cortical neurons of rats
QI Cui 1 , ZHANG Wei-Wei 2 , WANG Fei 1 , BAO Chao-Fei 1 , WANG Xin-Wei 1 , LI Xiao-Nan 1 , YU Xiao-Qing 1 , ZHOU Chen 1, *
1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. School of Basic Medical Science, Hebei University, Baoding 071000, China
Download: PDF(231 KB)  
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks    
 Interleukin-1β (IL-1β) is an important proinflammatory cytokine that plays a key role in injuries and diseases of the central nervous system (CNS). The voltage-gated Na+channel is the most important ion channel of neurons, and is essential for regenerative action potential (AP). The Na+ channel also contributes to many diseases of the brain. However, relations between IL-1β and central Na+ channels remain unreported. In this study, whole cell patch-clamp recording was used to investigate the acute effects of IL-1β (10 ng/mL) on voltage-dependent Na+ currents and AP of cultured cortical neurons from rats. Results showed that the half-activation voltage of Na+ channels and the threshold of AP, but not the amplitude, slope factor of activation, and inactivation properties, were affected by IL-1β. These data suggest that increased IL-1β in injury and disease may upregulate the excitability of neurons, and thereby exacerbate neurotoxicity.
Keywords Interleukin-1β      Cortical neurons      Voltage-dependent Na+ currents      Patch-clamp recording     
PACS:  Q424  
Corresponding Authors: ZHOU Chen   
About author: QI Cui
E-mail this article
E-mail Alert
Articles by authors
Cite this article:   
QI Cui,ZHANG Wei-Wei,WANG Fei,BAO Chao-Fei,WANG Xin-Wei,LI Xiao-Nan,YU Xiao-Q. Acute effects of IL-1β on sodium current in cortical neurons of rats. ZOOLOGICAL RESEARCH, 2011, 32(3): 323-328.
URL:     OR
Amaya F, Wang H, Costigan M, Allchorne AJ, Hatcher JP, Egerton J, Stean T, Morisset V, Grose D, Gunthorpe MJ, Chessell IP, Tate S, Green PJ, Woolf CJ. 2006. The voltage-gated sodium channel NaV1.9 is an effector of peripheral inflammatory pain hypersensitivity [J]. J Neurosci, 26 (50): 12852-12860.
Amir R, Argoff CE, Bennett GJ, Cummins TR, Durieux ME, Gerner P, Gold MS, Porreca F, Strichartz GR. 2006. The role of sodium channels in chronic inflammatory and neuropathic pain [J]. J Pain, 7: S1-S29.
Binshtok AM, Wang H, Zimmermann K, Amaya F, Vardeh D, Shi L, Brenner GJ, Ji RR, Bean BP, Woolf CJ, Samad TA. 2008. Nociceptors are interleukin-1β sensors
[J]. J Neurosci, 28 (52): 14062-14073.
Davis TH, Chen C, Isom LL. 2004. Sodium channel β1 subunits promote neurite outgrowth in cerebellar granule neurons [J]. J Biol Chem, 279: 51424-51432.
Diem R, Hobom M, Grötsch P, Kramer B, Bähr M. 2003. Interleukin-1β protects neurons via the interleukin-1 (IL-1) receptor-mediated Akt pathway and by IL-1 receptor-independent decrease of transmembrane currents in vivo [J]. Mol Cell Neurosci, 22: 487-500.
Fogal B, Hewett SJ. 2008. Interleukin-1β: A bridge between inflammation and excitotoxicity?  [J] J Neurochem, 106: 1-23.
Goldfarb M, Schoorlemmer J, Williams A, Diwakar S, Wang Q, Huang X, Giza J, Tchetchik D, Kelley K, Vega A, Matthews G, Rossi P, Ornitz DM, D’Angelo E. 2007. Fibroblast growth factor homologous factors control neuronal excitability through modulation of voltage-gated sodium channels [J]. Neuron, 55: 449-463.
Hains BC, Saab CY, Klein JP, Craner MJ, Waxman SG. 2004. Altered sodium channel expression in second-order spinal sensory neurons contributes to pain after peripheral nerve injury [J]. J Neurosci, 24: 4832-4839.
Liu L, Yang TM, Liedtke W, Simon SA. 2006. Chronic IL-1β potentiates voltage-dependent sodium currents in trigeminal nociceptive neurons [J]. J Neurophysiol, 95: 1478-1490.
Momin A, Wood JN. 2008. Sensory neuron voltage-gated sodium channels as analgesic drug targets [J]. Curr Opin Neurol, 18: 383-388.
O’Malley HA, Shreiner AB, Chen GH, Huffnagle GB, Isom LL. 2009. Loss of Na+ channel β2 subunits is neuroprotective in a mouse model of multiple sclerosis
[J]. Mol Cell Neurosci, 40: 143-155.
Plata-Salamán CR, ffrench-Mullen JMH. 1992. Interleukin-1β depresses calcium currents in CA1 hippocampal neurons at pathophysiological concentration [J]. Brain Res, 29: 221-223.
Rijkers K, Majoie HJ, Hoogland G, Kenis G, De Baets M, Vles JS. 2009. The role of interleukin-1 in seizures and epilepsy: A critical review [J]. Exp Neurol, 216: 258-271.
Rush AM, Dib-Hajj SD, Liu S, Cummins TR, Black JA, Waxman SG. 2006. A single sodium channel mutation produces hyper-or hypoexcitability in different types of neurons
[J]. Proc Natl Acad Sci USA, 103: 8245-8250.
Takeda M, Kitagawa J, Takahashi M,Matsumote S. 2008. Activation of interleukin-1β receptor suppresses the voltage-gated potassium currents in the small-diameter trigeminal ganglion neurons following peripheral inflammation [J]. Pain, 139: 594-602.
Yao C, Williams AJ, Hartings JA, Lu XCM, Tortella FC, Dave JR. 2005. Down-regulation of the sodium channel NaV1.1 α-subunit following focal ischemic brain injury in rats: In situ hybridization and immunohistochemical analysis [J]. Life Sci, 77: 1116-1129.
Zhang R, Yamada J, Hayashi Y, Wu Z, Koyama S, Nakanishi H. 2008. Inhibition of NMDA-induced outward currents by interleukin-1β in hippocampal neurons
[J]. Biochem Biophys Res Commun, 372: 816-820.
Zhang WW, Zhou C, Qi C, Bao CF, Jing JZ, Zhang LY, Han SE. 2009. Potassium currents in neurons of rats were reduced by IL-1β in time dependent manner.
[J]. Acta Biophys Sin, 25 (6): 415-422.[
张伟伟, 周辰, 齐翠, 鲍朝飞, 景军展, 张丽瑶, 韩素娥. 2009. 白介素时间依赖性地抑制大鼠皮层神经元钾电流. 生物物理学报, 25(6): 415-422.]
Zhou C, Tai C, Ye HH, Chen JG, Wang SQ, Chai Z. 2006a. Interleukin-1β downregulates the L-type Ca2+ channel activity by depressing the expression of channel protein in cortical neurons[J]. J Cell Physiol, 206: 799-806.
Zhou C, Ye HH, Wang SQ, Chai Z. 2006b. Interleukin-1β regulation of N- type Ca2+ channels in cortical neurons [J]. Neurosci Lett, 403: 181-185.

[1] ZHOU Chen*. Inhibition Effect of IL-1β on Calcium Channels Currents in Cultured Cortical Neurons of Rat[J]. ZOOLOGICAL RESEARCH, 2010, 31(1): 89-93.
[2] CHEN Qi-Cai,Philip H.S.JEN,WU Fei-Jian. Characteristics of Column Organization Sharpening Frequency Tunings of Bat Auditory Cortical Neurons[J]. ZOOLOGICAL RESEARCH, 2001, 22(3): 174-180.
Full text