ZOOLOGICAL RESEARCH 2011, 32(3) 323-328 DOI:   10.3724/SP.J.1141.2011.03323   ISSN: 0254-5853 CN: 53-1040/Q

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Keywords
Interleukin-1β
Cortical neurons
Voltage-dependent Na+ currents
Patch-clamp recording
Authors
PubMed

Acute effects of IL-1β on sodium current in cortical neurons of rats

QI Cui 1 , ZHANG Wei-Wei 2 , WANG Fei 1 , BAO Chao-Fei 1 , WANG Xin-Wei 1 , LI Xiao-Nan 1 , YU Xiao-Qing 1 , ZHOU Chen 1, *

1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. School of Basic Medical Science, Hebei University, Baoding 071000, China

Abstract

 Interleukin-1β (IL-1β) is an important proinflammatory cytokine that plays a key role in injuries and diseases of the central nervous system (CNS). The voltage-gated Na+channel is the most important ion channel of neurons, and is essential for regenerative action potential (AP). The Na+ channel also contributes to many diseases of the brain. However, relations between IL-1β and central Na+ channels remain unreported. In this study, whole cell patch-clamp recording was used to investigate the acute effects of IL-1β (10 ng/mL) on voltage-dependent Na+ currents and AP of cultured cortical neurons from rats. Results showed that the half-activation voltage of Na+ channels and the threshold of AP, but not the amplitude, slope factor of activation, and inactivation properties, were affected by IL-1β. These data suggest that increased IL-1β in injury and disease may upregulate the excitability of neurons, and thereby exacerbate neurotoxicity.

Keywords Interleukin-1β   Cortical neurons   Voltage-dependent Na+ currents   Patch-clamp recording  
Received 2010-09-25 Revised 2011-01-28 Online: 2011-06-22 
DOI: 10.3724/SP.J.1141.2011.03323
Fund:
Corresponding Authors: ZHOU Chen
Email: zezhou@sina.com
About author: QI Cui

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