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ZOOLOGICAL RESEARCH    2011, Vol. 32 Issue (3) : 323-328     DOI: 10.3724/SP.J.1141.2011.03323
Articles |
Acute effects of IL-1β on sodium current in cortical neurons of rats
QI Cui 1 , ZHANG Wei-Wei 2 , WANG Fei 1 , BAO Chao-Fei 1 , WANG Xin-Wei 1 , LI Xiao-Nan 1 , YU Xiao-Qing 1 , ZHOU Chen 1, *
1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. School of Basic Medical Science, Hebei University, Baoding 071000, China
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 Interleukin-1β (IL-1β) is an important proinflammatory cytokine that plays a key role in injuries and diseases of the central nervous system (CNS). The voltage-gated Na+channel is the most important ion channel of neurons, and is essential for regenerative action potential (AP). The Na+ channel also contributes to many diseases of the brain. However, relations between IL-1β and central Na+ channels remain unreported. In this study, whole cell patch-clamp recording was used to investigate the acute effects of IL-1β (10 ng/mL) on voltage-dependent Na+ currents and AP of cultured cortical neurons from rats. Results showed that the half-activation voltage of Na+ channels and the threshold of AP, but not the amplitude, slope factor of activation, and inactivation properties, were affected by IL-1β. These data suggest that increased IL-1β in injury and disease may upregulate the excitability of neurons, and thereby exacerbate neurotoxicity.
Keywords Interleukin-1β      Cortical neurons      Voltage-dependent Na+ currents      Patch-clamp recording     
PACS:  Q424  
Corresponding Authors: ZHOU Chen   
About author: QI Cui
Issue Date: 22 June 2011
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QI Cui,ZHANG Wei-Wei,WANG Fei,BAO Chao-Fei,WANG Xin-Wei,LI Xiao-Nan,YU Xiao-Q. Acute effects of IL-1β on sodium current in cortical neurons of rats[J]. ZOOLOGICAL RESEARCH,2011, 32(3): 323-328.
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