Zoological Research ›› 2008, Vol. 29 ›› Issue (6): 592-598.doi: 10.3724/SP.J.1141.2008.06592

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Molecular Cloning and Expression Analysis of FTZ-F1 in the Half-smooth Tongue-sole, Cynoglossus semilaevis

DENG Si-ping 1,2, CHEN Song-lin 2, LIU Ben-wei 2   

  1. 1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China; 2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China
  • Received:2008-07-15 Revised:1900-01-01 Online:2008-12-22 Published:2008-12-22
  • Contact: CHEN Song-lin

Abstract:

To investigate the expression characteristics of sex related gene of FTZ-F1 in the half-smooth tongue-sole (Cynoglossus semilaevis), the homologue FTZ-F1 (hsFTZ-F1) full-length cDNA was isolated from the testis by homologous cloning, and the cDNA included the open reading frame and a 66 bp 5'-UTR, along with a 1 619 bp 3'-UTR, encoding a predicted 485 amino acid protein. Sequence, tissue distribution and phylogenic analyses of the FTZ-F1 showed that the hsFTZ-F1 belonged to SF-1/Ad4BP group. The hsFTZ-F1 transcripts were highly abundant in the gonads, kidneys, brain and head-kidneys, but weakly in other tissues. However, the expression level in the brain and head-kidney of female was highly abundant than in the male. The hsFTZ-F1 expression was highly abundant in the embryo than in the larvae, which suggested that the hsFTZ-F1 may be involved in the organogenesis in the tongue sole.