Review of the genus Brachytarsophrys (Anura: Megophryidae), with revalidation of Brachytarsophrys platyparietus and description of a new species from China
Yao Li, Dan-Dan Zhang, Zhi-Tong Lyu, Jian Wang, Yu-Long Li, Zu-Yao Liu, Hong-Hui Chen, Ding-Qi Rao, Zhi-Fang Jin, Chang-You Zhang, Ying-Yong Wang
2020 Vol. 41 (2): 105-122
Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb
Ning Zheng, Zhi-Zhong Wang, Song-Wei Wang, Fang-Jia Yang, Xu-Tao Zhu, Chen Lu, Anne Manyande, Xiao-Ping Rao, Fu-Qiang Xu
2020 Vol. 41 (2): 148-156
      18 March 2020, Volume 41 Issue 2 Previous Issue   
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    Contents
    Zoological Research. 2020, 41 (2): 97-212.  
    Abstract ( 18 )   RICH HTML PDF (284120KB) ( 18 )   Save
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    Review
    Deciphering the puzzles of dog domestication
    Zhe Zhang, Saber Khederzadeh, Yan Li
    Zoological Research. 2020, 41 (2): 97-104.   DOI: 10.24272/j.issn.2095-8137.2020.002
    Abstract ( 384 )   RICH HTML ( 217 )   PDF (210KB) ( 226 )   Save

    The domestic dog, as a highly successful domestication model, is well known as a favored human companion. Exploring its domestication history should provide great insight into our understanding of the prehistoric development of human culture and productivity. Furthermore, investigation on the mechanisms underpinning the morphological and behavioral traits associated with canid domestication syndrome is of significance not only for scientific study but also for human medical research. Current development of a multidisciplinary canine genome database, which includes enormous omics data, has substantially improved our understanding of the genetic makeup of dogs. Here, we reviewed recent advances associated with the original history and genetic basis underlying environmental adaptations and phenotypic diversities in domestic dogs, which should provide perspectives on improving the communicative relationship between dogs and humans.

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    Articles
    Review of the genus Brachytarsophrys (Anura: Megophryidae), with revalidation of Brachytarsophrys platyparietus and description of a new species from China
    Yao Li, Dan-Dan Zhang, Zhi-Tong Lyu, Jian Wang, Yu-Long Li, Zu-Yao Liu, Hong-Hui Chen, Ding-Qi Rao, Zhi-Fang Jin, Chang-You Zhang, Ying-Yong Wang
    Zoological Research. 2020, 41 (2): 105-122.   DOI: 10.24272/j.issn.2095-8137.2020.033
    Abstract ( 245 )   RICH HTML ( 24 )   PDF (185567KB) ( 301 )   Save

    The genus-level recognition of monophyletic short-legged toads (Brachytarsophrys) has been recently implicated in the taxonomic debate of Megophrys sensu lato. In the present study, Brachytarsophrys is reasonably regarded as a distinct genus based on significant morphological differentiations and recent molecular analyses. Furthermore, a comprehensive review of this genus is performed, with two species groups proposed based on morphological differences and phylogenetic relationships. Particularly, Brachytarsophrys platyparietus is removed as a synonym of Brachytarsophrys carinense and considered a valid species due to significant genetic divergence and distinct morphological differences. In addition, a new species, Brachytarsophrys orientalis sp. nov., is described based on a series of specimens collected from southeastern China. This work takes the member species of the genus Brachytarsophrys to seven, suggesting that the diversity of Brachytarsophrys is underestimated. In addition, the genus levels of other monophyletic groups within the subfamily Megophryinae are discussed.

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    Mudskipper interleukin-34 modulates the functions of monocytes/macrophages via the colony-stimulating factor-1 receptor 1
    Hai-Yu Shen, Yan Zhou, Qian-Jin Zhou, Ming-Yun Li, Jiong Chen
    Zoological Research. 2020, 41 (2): 123-137.   DOI: 10.24272/j.issn.2095-8137.2020.026
    Abstract ( 93 )   RICH HTML ( 17 )   PDF (9265KB) ( 83 )   Save

    Interleukin-34 (IL-34) is a novel cytokine that plays an important role in innate immunity and inflammatory processes by binding to the colony-stimulating factor-1 receptor (CSF-1R). However, information on the function of IL-34 in fish remains limited. In the present study, we identified an IL-34 homolog from mudskippers (Boleophthalmus pectinirostris). In silico analysis showed that the mudskipper IL-34 (BpIL-34) was similar to other known IL-34 variants in sequence and structure and was most closely related to an orange-spotted grouper (Epinephelus coioides) homolog. BpIL-34 transcripts were constitutively expressed in various tissues, with the highest level of expression found in the brain. Edwardsiella tarda infection significantly up-regulated the mRNA expression of BpIL-34 in the mudskipper tissues. The recombinant mature BpIL-34 peptide (rBpIL-34) was purified and used to produce anti-rBpIL-34 IgG. Western blot analysis combined with PNGase F digestion revealed that native BpIL-34 in monocytes/macrophages (MOs/MФs) was N-glycosylated. In vitro, rBpIL-34 treatment enhanced the phagocytotic and bactericidal activity of mudskipper MOs/MФs, as well as the mRNA expression of pro-inflammatory cytokines like tumor necrosis factor α (BpTNF-α) and BpIL-1β in these cells. Furthermore, the knockdown of mudskipper CSF-1R1 (BpCSF-1R1), but not mudskipper BpCSF-1R2, significantly inhibited the rBpIL-34-mediated enhanced effect on MO/MФ function. In conclusion, our results indicate that mudskipper BpIL-34 modulates the functions of MOs/MФs via BpCSF-1R1.

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    Comparative analysis of diverse toxins from a new pharmaceutical centipede, Scolopendra mojiangica
    Zi-Chao Liu, Jin-Yang Liang, Xin-Qiang Lan, Tao Li, Jia-Rui Zhang, Fang Zhao, Geng Li, Pei-Yi Chen, Yun Zhang, Wen-Hui Lee, Feng Zhao
    Zoological Research. 2020, 41 (2): 138-147.   DOI: 10.24272/j.issn.2095-8137.2020.019
    Abstract ( 131 )   RICH HTML ( 5 )   PDF (2984KB) ( 114 )   Save

    As the oldest venomous animals, centipedes use their venom as a weapon to attack prey and for protection. Centipede venom, which contains many bioactive and pharmacologically active compounds, has been used for centuries in Chinese medicine, as shown by ancient records. Based on comparative analysis, we revealed the diversity of and differences in centipede toxin-like molecules between Scolopendra mojiangica, a substitute pharmaceutical material used in China, and S. subspinipes mutilans. More than 6 000 peptides isolated from the venom were identified by electrospray ionization-tandem mass spectrometry (ESI-MS/MS) and inferred from the transcriptome. As a result, in the proteome of S. mojiangica, 246 unique proteins were identified: one in five were toxin-like proteins or putative toxins with unknown function, accounting for a lower percentage of total proteins than that in S. mutilans. Transcriptome mining identified approximately 10 times more toxin-like proteins, which can characterize the precursor structures of mature toxin-like peptides. However, the constitution and quantity of the toxin transcripts in these two centipedes were similar. In toxicity assays, the crude venom showed strong insecticidal and hemolytic activity. These findings highlight the extensive diversity of toxin-like proteins in S. mojiangica and provide a new foundation for the medical-pharmaceutical use of centipede toxin-like proteins.

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    Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb
    Ning Zheng, Zhi-Zhong Wang, Song-Wei Wang, Fang-Jia Yang, Xu-Tao Zhu, Chen Lu, Anne Manyande, Xiao-Ping Rao, Fu-Qiang Xu
    Zoological Research. 2020, 41 (2): 148-156.   DOI: 10.24272/j.issn.2095-8137.2020.020
    Abstract ( 130 )   RICH HTML ( 8 )   PDF (32663KB) ( 110 )   Save

    The accessory olfactory bulb (AOB), located at the posterior dorsal aspect of the main olfactory bulb (MOB), is the first brain relay of the accessory olfactory system (AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system (MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by single-color labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis (BST), anterior cortical amygdalar area (ACo), medial amygdaloid nucleus (MeA), and posteromedial cortical amygdaloid area (PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.

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    Reports
    Exploring the reproductive ecology of the tropical semifossorial snake Ninia atrata
    Teddy Angarita-Sierra, Cesar Alejandro López-Hurtado
    Zoological Research. 2020, 41 (2): 157-171.   DOI: 10.24272/j.issn.2095-8137.2020.015
    Abstract ( 141 )   RICH HTML ( 3 )   PDF (12512KB) ( 139 )   Save

    Based on histological analyses and field studies, this research describes the reproductive ecology of a population of Ninia atrata snakes inhabiting an oil palm plantation. Furthermore, through a multivariate approach, we explored the main drivers of reproductive output in N. atrata. Results showed that prey abundance and food intake were crucial variables contributing to reproductive output. Multiple linear regression models showed that neonates had high sensitivity (R2=55.29%) to extreme changes in climate, which was strongly related to slug and snail abundance variability and microhabitat quality. Reproductive cycles were markedly different between the sexes, being continuous in males and cyclical in females. Despite this variation, reproductive cycles at the population level were seasonal semi-synchronous. Constant recruitment of neonates all year, multiple clutches, high mating frequency, and continuous sperm production characterized the reproductive phenology of N. atrata. In addition, a significant number of previtellogenic females presented oviductal sperm as well as uterine scars, suggesting a high precocity in the species. The main drivers of reproductive output also differed between the sexes. In females, clutch size and secondary follicle variability were highly related to stomach bolus volume, fat body area, and body mass. In males, height of piles of palm leaves and body mass, rather than intrinsic reproductive traits, were the main drivers of sperm production. Nevertheless, in both cases, the relationship between body mass, prey abundance, and food intake suggests that N. atrata follows the income breeding strategy to compensate for reproductive costs and to maximize fitness.

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    Morphological and ecological divergence in two populations of European glass lizard, Pseudopus apodus (Squamata: Anguidae)
    Olga Jovanović Glavaš, Paula Počanić, Vanja Lovrić, Lorena Derežanin, Zoran Tadić, Duje Lisičić
    Zoological Research. 2020, 41 (2): 172-181.   DOI: 10.24272/j.issn.2095-8137.2020.025
    Abstract ( 144 )   RICH HTML ( 3 )   PDF (1706KB) ( 113 )   Save

    The European glass lizard, Pseudopus apodus (Pallas, 1775), is a large, legless lizard with wide distribution across south-eastern Europe and eastern and central Asia. To date, morphological diversification among populations on a geographically small scale has not yet been reported in this lizard. Thus, we investigated the morphological variations and corresponding differences in habitat utilization between two populations of P. apodus inhabiting the same biogeographical zone within a relatively close geographic area. We hypothesized that minor differences in habitat could induce a significant level of morphological differentiation, thus indicating morphological plasticity in this species on a small geographical scale. We sampled 164 individuals (92 from the Croatian mainland and 72 from the island of Cres). Results showed that P. apodus indeed exhibited morphological differences between populations in the same biogeographical zone within a relatively close geographic area, with the Cres Island individuals being generally larger than the individuals from the mainland. Some ecological characteristics were similar in both populations (e.g., soil temperature, distance to hiding place), whereas others were distinct (e.g., air temperature and humidity). In addition, vegetation cover differed between the two sites, with more vegetation present on the mainland than on the island. Furthermore, the Cres Island population showed clear sexual dimorphism, which was absent in the mainland population.

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    Letters to the editor
    Spatial and temporal patterns of amphibian species richness on Tianping Mountain, Hunan Province, China
    Wen-Bo Zhu, Chun-Lin Zhao, Chun-Lin Liao, Bei Zou, Dan Xu, Wei Zhu, Tian Zhao, Jian-Ping Jiang
    Zoological Research. 2020, 41 (2): 182-187.   DOI: 10.24272/j.issn.2095-8137.2020.017
    Abstract ( 138 )   RICH HTML ( 7 )   PDF (793KB) ( 143 )   Save

    Exploring species richness patterns across space and time can help in understanding species distribution and in formulating conservation strategies. Among taxa, amphibians are of utmost importance as they are highly sensitive to environmental changes due to their unique life histories (Zhong et al., 2018). Here, we investigated the spatial and temporal patterns of amphibian species richness on Tianping Mountain in China. Specifically, we established 10 transects at low to high elevations, and sampled amphibians in April, June, August, and October 2017. Our results demonstrated that amphibian species composition and richness varied significantly at both spatial and temporal scales and were associated with gradients of environmental change in microhabitats on Tianping Mountain.

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    A new species of the genus Amolops (Anura: Ranidae) from Yunnan, China
    Yu-Lu Gan, Guo-Hua Yu, Zheng-Jun Wu
    Zoological Research. 2020, 41 (2): 188-193.   DOI: 10.24272/j.issn.2095-8137.2020.018
    Abstract ( 103 )   RICH HTML ( 6 )   PDF (5558KB) ( 156 )   Save

    A new species of the genus Amolops, Amolops tuanjieensis sp. nov., is described from Yunnan, China. The new species can be distinguished by the following characters: dorsolateral folds present; dorsal and ventral surfaces smooth; top of head and dorsum brown-red with irregular gray and dark spots; flank green; side of head black, from tip of snout, diffusing posteriorly to axilla, continuing as black streak below edge of dorsolateral fold; SVL 39.5–40.4 mm in males, 56.8–60.7 mm in females; tympanum distinct; supratympanic fold indistinct; vomerine teeth in two oblique rows between choanae, closer to each other than choanae; vocal sacs present; nuptial pads present; outer metatarsal tubercle absent, supernumerary tubercles absent; all fingertips expanded into discs; limbs dorsally brown with dark brown bars and irregular dark brown blotches.

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    A new species of the genus Sinomicrurus (Serpentes: Elapidae) from China and Vietnam
    Qin Liu, Jiao-Wen Yan, Shao-Bing Hou, Ping Wang, Sang Ngoc Nguyen, Robert W. Murphy, Jing Che, Peng Guo
    Zoological Research. 2020, 41 (2): 194-198.   DOI: 10.24272/j.issn.2095-8137.2020.023
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    A new species of Sinomicrurus Slowinski, Boundy, and Lawson, 2001 is described herein based on a series of specimens. The new species, Sinomicrurus peinani sp. nov., occurs in southern China and northern Vietnam. Sinomicrurus peinani sp. nov. is distinguished from its congeners by the following combination of characters: (1) 30–35 black cross-bands on body and tail; (2) 13 dorsal scale rows throughout, all smooth; (3) white belly with black cross-bands or irregular spots; (4) broad white transverse bar on top of head with inverted V-shaped anterior margin, white bar wider than anterior black bar; and (5) frontal V-like, 1.3 times as long as wide. In addition, new occurrences of S. houi in Guangxi, China, and Vietnam are discussed.

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    Preliminary study on fine structures of subcortical nuclei in rhesus monkeys by ex vivo 9.4 T MRI
    Huan-Zhi Chen, Hong-Yi Yang, Kai Zhong, Jia-Li Li
    Zoological Research. 2020, 41 (2): 199-202.   DOI: 10.24272/j.issn.2095-8137.2020.013
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    Changes in fine structures of the brain over a life span can have robust effects on neural activity and brain function, which both play crucial roles in neurodegenerative diseases. Clinically, however, low-resolution MRI only provides limited information about fine brain structures. Here, using high-resolution 9.4 T MRI, we established a set of structural images and explored the fine structures of the claustrum, hippocampus, amygdala complex, and subregions of the amygdala complex (BLA, including lateral, basal, and accessory basal subnuclei) in rhesus macaque (Macaca mulatta) brains. Based on these high-resolution images, we were able to discriminate the subregional boundaries accurately and, at the same time, obtain the volume of each brain nuclei. Thus, advanced high-resolution 9.4 T MRI not only provides a new strategy for early diagnosis of neurodegenerative diseases, but also provides the ability to observe fine structural changes in the brain across a life span.

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    Hyperactive reactive oxygen species impair function of porcine Sertoli cells via suppression of surface protein ITGB1 and connexin-43
    Dan-Chen Zhang, Rong Chen, Yi-Hui Cai, Jing-Jing Wang, Chang Yin, Kang Zou
    Zoological Research. 2020, 41 (2): 203-207.   DOI: 10.24272/j.issn.2095-8137.2020.024
    Abstract ( 79 )   RICH HTML ( 10 )   PDF (2727KB) ( 64 )   Save

    Gap junctions regulate intercellular communication between Sertoli cells and germ cells in male testes and play vital functions in spermatogenesis. Many factors in animal breeding and husbandry can induce oxidative stress, which can impair the testis microenvironment and male animal fertility. However, the underlying mechanisms are largely unknown. Recently, we identified that androgen signals promote the expression of connexin-43 (Cx43), a key component of gap junctions, to regulate spermatogenesis. Thus, we asked whether hyperactive reactive oxygen species (ROS) can impair gap junctions by interfering with Cx43 in porcine testes. Using a porcine Sertoli cell in vitro system, we found that hyperactive ROS caused extensive apoptosis in Sertoli cells, remarkable decrease in Cx43 expression, and failed maintenance of co-cultured spermatogonial stem cells (SSCs), indicating that ROS impaired the function of Sertoli cells and promoted loss of SSCs. This observation provides a possible mechanism for the impact of ROS on fertility of male animals.

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    The anatomy of the skin of the Chinese tree shrew is very similar to that of human skin
    Jing Zhang, Rong-Can Luo, Xiao-Yong Man, Long-Bao Lv, Yong-Gang Yao, Min Zheng
    Zoological Research. 2020, 41 (2): 208-212.   DOI: 10.24272/j.issn.2095-8137.2020.028
    Abstract ( 143 )   RICH HTML ( 12 )   PDF (13553KB) ( 122 )   Save

    The Chinese tree shrew (Tupaia belangeri chinensis) is a small mammal closely related to primates. It has a small body size, low maintenance cost, and a relatively short reproductive cycle, all of which has made it the ideal model for the study of a variety of human diseases. In this study, we compared the anatomy of the skin of the Chinese tree shrew with that of the rhesus macaque, mouse and human, with the intention of providing the basic data required for the creation of skin disease models using this animal. Paraffin sections, hematoxylin-eosin (H&E) staining, masson staining and immunohistochemical techniques were used to examine the dorsal skin structure of the Chinese tree shrew. The epidermis was shown to be composed of 1–2 layers of cells. There were hair follicles, sebaceous glands and sweat glands in the dermis and the subcutaneous tissue, with apocrine glands being more common than eccrine glands. Both Keratin5 (KRT5) and Keratin10 (KRT10) were expressed in the skin of the Chinese tree shrew, with a localization in the cytoplasm. Overall, the skin morphology and histology of the Chinese tree shrew was basically the same as that of the human. We propose that the Chinese tree shrew has a strong potential to be used for creating animal models to help elucidate the molecular mechanisms underlying a variety of skin diseases.

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Vol 41, No 2  (18 March 2020)

Indexed by SCI-E
2018 Impact Factor 1.556
52/170 Zoology (Q2)
2019 Journal Citation Reports®

Bimonthly, Since 1980
Editor-in-Chief: Yong-Gang Yao
ISSN: 2095-8137
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