How many species of Apodemus and Rattus occur in China? A survey based on mitochondrial cyt b and morphological analyses
Shao-Ying Liu, Kai He, Shun-De Chen, Wei Jin, Robert W. Murphy, Ming-Kun Tang, Rui Liao, Feng-Jun Li
2018 Vol. 39 (5): 309-320
A new genus of Asiatic short-tailed shrew (Soricidae, Eulipotyphla) based on molecular and morphological comparisons
Kai He, Xing Chen, Peng Chen, Shui-Wang He, Feng Cheng, Xue-Long Jiang, Kevin Campbell
2018 Vol. 39 (5): 321-334
      18 September 2018, Volume 39 Issue 5 Previous Issue   
    Species delimitation based on diagnosis and monophyly, and its importance for advancing mammalian taxonomy
    Eliécer E. Gutiérrez, Guilherme S. T. Garbino
    Zoological Research. 2018, 39 (5): 301-308.   DOI: 10.24272/j.issn.2095-8137.2018.037
    Abstract ( 36 )   RICH HTML PDF (281KB) ( 128 )

    A recently proposed taxonomic classification of extant ungulates sparked a series of publications that criticize the Phylogenetic Species Concept (PSC) claiming it to be a particularly poor species concept. These opinions reiteratively stated that (1) the two fundamental elements of the "PSC", i.e., monophyly and diagnosability, do not offer objective criteria as to where the line between species should be drawn; and (2) that extirpation of populations can lead to artificial diagnosability and spurious recognitions of species. This sudden eruption of criticism against the PSC is misleading. Problems attributed to the PSC are common to most approaches and concepts that modern systematists employ to establish species boundaries. The controversial taxonomic propositions that sparked criticism against the PSC are indeed highly problematic, not because of the species concept upon which they are based, but because no evidence (whatsoever) has become public to support a substantial portion of the proposed classification. We herein discuss these topics using examples from mammals. Numerous areas of biological research rest upon taxonomic accuracy (including conservation biology and biomedical research); hence, it is necessary to clarify what are (and what are not) the real sources of taxonomic inaccuracy.

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    How many species of Apodemus and Rattus occur in China? A survey based on mitochondrial cyt b and morphological analyses
    Shao-Ying Liu, Kai He, Shun-De Chen, Wei Jin, Robert W. Murphy, Ming-Kun Tang, Rui Liao, Feng-Jun Li
    Zoological Research. 2018, 39 (5): 309-320.   DOI: 10.24272/j.issn.2095-8137.2018.053
    Abstract ( 62 )   RICH HTML PDF (7935KB) ( 144 )
    Apodemus (mice) and Rattus (rats) are the top rodent reservoirs for zoonoses in China, yet little is known about their diversity. We reexamined the alpha diversity of these two genera based on a new collection of specimens from China and their cyt b sequences in GenBank. We also tested whether species could be identified using external and craniodental measurements exclusively. Measurements from 147 specimens of Apodemus and 236 specimens of Rattus were used for morphological comparisons. We analysed 74 cyt b sequences of Apodemus and 100 cyt b sequences of Rattus to facilitate phylogenetic estimations. Results demonstrated that nine species of Apodemus and seven species of Rattus, plus a new subspecies of Rattus nitidus, are distributed in China. Principal component analysis using external and craniodental measurements revealed that measurements alone could not separate the recognized species. The occurrence of Rattus pyctoris in China remains uncertain.
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    A new genus of Asiatic short-tailed shrew (Soricidae, Eulipotyphla) based on molecular and morphological comparisons
    Kai He, Xing Chen, Peng Chen, Shui-Wang He, Feng Cheng, Xue-Long Jiang, Kevin Campbell
    Zoological Research. 2018, 39 (5): 321-334.   DOI: 10.24272/j.issn.2095-8137.2018.058
    Abstract ( 92 )   RICH HTML PDF (3564KB) ( 69 )
    Blarinellini is a tribe of soricine shrews comprised of nine fossil genera and one extant genus. Blarinelline shrews were once widely distributed throughout Eurasia and North America, though only members of the Asiatic short-tailed shrew genus Blarinella currently persist (mostly in southwestern China and adjacent areas). Only three forms of Blarinella have been recognized as either species or subspecies. However, recent molecular studies indicated a strikingly deep divergence within the genus, implying the existence of a distinct genus-level lineage. We sequenced the complete mitochondrial genomes and one nuclear gene of three Asiatic short-tailed and two North American shrews and analyzed them morphometrically and morphologically. Our molecular analyses revealed that specimens ascribed to B. griselda formed two deeply diverged lineages, one a close relative to B. quadraticauda, whereas the other — comprised of topotype specimens from southern Gansu — diverged from other Blarinella in the middle Miocene (ca. 18.2 million years ago (Ma), 95% confidence interval=13.4–23.6 Ma). Although the skulls were similarly shaped in both lineages, we observed several diagnostic characteristics, including the shape of the upper P4. In consideration of the molecular and morphological evidence, we recognize B. griselda as the sole species of a new genus, namely, Pantherina gen. nov. Interestingly, some characteristics of Pantherina griselda are more similar to fossil genera, suggesting it represents an evolutionarily more primitive form than Blarinella. Recognition of this new genus sheds light on the systematics and evolutionary history of the tribe Blarinellini throughout Eurasia and North America. 
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    Taxonomic revision of the genus Mesechinus (Mammalia: Erinaceidae) with description of a new species
    Huai-Sen Ai, Kai He, Zhong-Zheng Chen, Jia-Qi Li, Tao Wan, Quan Li, Wen-Huan Nie, Jin-Huan Wang, Wei-Ting Su, Xue-Long Jiang
    Zoological Research. 2018, 39 (5): 335-347.   DOI: 10.24272/j.issn.2095-8137.2018.034
    Abstract ( 55 )   RICH HTML PDF (25432KB) ( 391 )
    Hedgehogs in the genus Mesechinus (Family Erinaceidae), which include two currently recognized species (M. dauuricus and M. hughi), are distributed from northeast Mongolia to the upper Amur Basin in Russia and adjacent areas in northeast and northern China. In recent years, a population of Mesechinus hedgehogs was discovered from Mt. Gaoligong, southwestern Yunnan, China, far from the known distribution range of the genus. Furthermore, these hedgehogs are the only known population to be distributed at elevations higher than 2 100 m and in sympatry with gymnures. To evaluate the taxonomic status of these hedgehogs, we examined specimens representing Mesechinus taxa in China and further conducted morphometric and karyotypic analyses. Our results supported the existence of four species in China. Specifically, we identified the hedgehogs from Mt. Gaoligong as a new species, Mesechinus wangi sp. nov., and recognized M. miodon, previously considered as a synonym of either M. dauuricus or M. hughi, as a distinct species. Interestingly, we observed a supernumerary M4 on all specimens of Mesechinus wangi sp. nov., which is an extremely rare event in the evolution of mammalian dentition.
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    Karyotypes of field mice of the genus Apodemus (Mammalia: Rodentia) from China
    Masaharu Motokawa, Yi Wu, Masashi Harada, Yuta Shintaku, Xue-Long Jiang, Yu-Chun Li
    Zoological Research. 2018, 39 (5): 348-355.   DOI: 10.24272/j.issn.2095-8137.2018.054
    Abstract ( 36 )   RICH HTML PDF (595KB) ( 38 )
    Karyotypes of four Chinese species of field mice of the genus Apodemus were examined, including Apodemus chevrieri (diploid chromosome number, 2n=48, fundamental number of autosomal arms, FNa=56), A. draco (2n=48, FNa=48), A. ilex (2n=48, FNa=48), and A. latronum (2n=48, FNa=48). Karyotypes of A. chevrieri, A. draco, and A. ilex are reported here for the first time, providing useful information for their species taxonomy. Determining the karyotypes of all species of Apodemus in Asia, both in this and previous studies, provides a solid overview of the chromosome evolution and species differentiation of the genus in East Asia. In addition to allopatric speciation, chromosome rearrangements likely played an important role in the formation of the four Apodemus species groups as well as speciation within each group in East Asia. For example, increased centromeric heterochromatin in A. latronum may have contributed to the post-mating reproductive isolation from the A. draco-A. ilex-A. semotus clade.
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    Species identification of crested gibbons (Nomascus) in captivity in China using karyotyping- and PCR-based approaches
    Wen-Hui Nie, Jin-Huan Wang, Wei-Ting Su, Yu Hu, Shui-Wang He, Xue-Long Jiang, Kai He
    Zoological Research. 2018, 39 (5): 356-363.   DOI: 10.24272/j.issn.2095-8137.2018.036
    Abstract ( 21 )   RICH HTML PDF (9321KB) ( 196 )
    Gibbons and siamangs (Hylobatidae) are well-known for their rapid chromosomal evolution, which has resulted in high speciation rate within the family. On the other hand, distinct karyotypes do not prevent speciation, allowing interbreeding between individuals in captivity, and the unwanted hybrids are ethically problematic as all gibbon species are endangered or critically endangered. Thus, accurate species identification is crucial for captive breeding, particularly in China where studbooks are unavailable. Identification based on external morphology is difficult, especially for hybrids, because species are usually similar in appearance. In this study, we employed G-banding karyotyping and fluorescence in situ hybridization (FISH) as well as a PCR-based approach to examine karyotypic characteristics and identify crested gibbons of the genus Nomascus from zoos and nature reserves in China. We characterized and identified five karyotypes from 21 individuals of Nomascus. Using karyotypes and mitochondrial and nuclear genes, we identified three purebred species and three hybrids, including one F2 hybrid between N. gabriellae and N. siki. Our results also supported that N. leucogenys and N. siki shared the same inversion on chromosome 7, which resolves arguments from previous studies. Our results demonstrated that both karyotyping and DNA-based approaches were suitable for identifying purebred species, though neither was ideal for hybrid identification. The advantages and disadvantages of both approaches are discussed. Our results further highlight the importance of animal ethics and welfare, which are critical for endangered species in captivity.
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     Impacts of late Quaternary environmental change on the long-tailed ground squirrel (Urocitellus undulatus) in Mongolia
    Bryan S. McLean, Batsaikhan Nyamsuren, Andrey Tchabovsky, Joseph A. Cook
    Zoological Research. 2018, 39 (5): 364-372.   DOI: 10.24272/j.issn.2095-8137.2018.042
    Abstract ( 37 )   RICH HTML PDF (535KB) ( 99 )

    Impacts of Quaternary environmental changes on mammal faunas of central Asia remain poorly understood due to a lack of geographically comprehensive phylogeographic sampling for most species. To help address this knowledge gap, we conducted the most extensive molecular analysis to date of the long-tailed ground squirrel (Urocitellus undulatus Pallas 1778) in Mongolia, a country that comprises the southern core of this species’ range. Drawing on material from recent collaborative field expeditions, we genotyped 128 individuals at 2 mitochondrial genes (cytochrome b and cytochrome oxidase I; 1 797 bp total). Phylogenetic inference supports the existence of two deeply divergent infraspecific lineages (corresponding to subspecies U. u. undulatus and U. u. eversmanni), a result in agreement with previous molecular investigations but discordant with patterns of range-wide craniometric and external phenotypic variation. In the widespread western eversmanni lineage, we recovered geographically-associated clades from the: (a) Khangai, (b) Mongolian Altai, and (c) Govi Altai mountain ranges. Phylogeographic structure in U. u. eversmanni is consistent with an isolation-by-distance model; however, genetic distances are significantly lower than among subspecies, and intra-clade relationships are largely unresolved. The latter patterns, as well as the relatively higher nucleotide polymorphism of populations from the Great Lakes Depression of northwestern Mongolia, suggest a history of range shifts into these lowland areas in response to Pleistocene glaciation and environmental change, followed by upslope movements and mitochondrial lineage sorting with Holocene aridification. Our study illuminates possible historical mechanisms responsible for U. undulatus genetic structure and contributes to a framework for ongoing exploration of mammalian response to past and present climate change in central Asia.

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Volume 39 Issue 5
18 September 2018
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