Zoological Research ›› 2011, Vol. 32 ›› Issue (1): 66-69.doi: 10.3724/SP.J.1141.2011.01066

• Reports • Previous Articles     Next Articles

Preliminary investigation of viruses to the wild tree shrews (Tupaia belangeri chinese)

WANG Xin-Xing, LI Jing-Xiao, WANG Wen-Guang, SUN Xiao-Mei, HE Chun-Yan, DAI Jie-Jie*   

  1. Institute of Medical Biology, Chinese Academy of Medical Sciences/Peking Union Medical College, Kunming Yunnan 650118, China
  • Received:2010-12-01 Revised:2011-01-26 Online:2011-02-22 Published:2011-02-22
  • Contact: DAI Jie-Jie E-mail:djj@imbcams.com.cn
  • About author:WANG Xin-Xing(1986-),E-mail:wxxing666@163.com

Abstract:

 Virological testing and monitoring is a fundamental part of quality control of experimental animals. However, there are few papers regarding the spectrum and status of natural infection in wild tree shrews with human and animal pathogenic viruses. Using enzyme-linked immunosorbent adsorption assay (ELISA), we tested sixty wild tree shrews captured from Qinglong, an outskirt region of Kunming, Yunnan Province, China for eleven viruses, including herpes simplex virus, coxsackie virus, influenza virus, HAV, HBV, HCV, HDV, dengue virus, hemorrhagic fever virus and measles virus. Our results showed that, in the serum samples, 22/60 (36.7%) and 1/60 (1.67%) were antibody positive for herpes simplex virus and coxsackie virus, respectively, and 4/60 (6.7%) were antigen positive for rotavirus in the feces. The remaining species of viruses were negative in these tree shrews. Based on these results, we propose that herpes simplex virus, coxsackie virus and cotavirus should be listed as top priority for routine virological monitoring of tree shrews.

CLC Number: 

  • Q95-33
Chen J, Dai JJ, Sun XM. 2008. Advances in the use of tree shrews as animal models for hepatitis research[J]. Chin J Compar Med 18(2): 59-62.
[
陈瑾, 代解杰, 孙晓梅. 2008. 树鼩肝炎动物模型的研究进展.中国比较医学杂志. 18(2): 59-62.]
Daral G, Zöller L, Matz B, Schwaier A, Flügel RM, Munk K. 1980. Experimental infection and the state of viral latency of adult Tupaia with herpes simplex virus tape 1 and 2 and infection of juvenile Tupaia with temperature-sensitive mutants of HSV type 2[J]. Arch Virol, 65: 311-318.
中华人民共和国国家质量监督检验检疫总局. 2002. GB/T14926.1-14926.41-2001[S]. 实验动物微生物学检验方法. 北京:中国国家标准化管理委员会.
中华人民共和国国家质量监督检验检疫总局. 2002. GB14922.2-2001[S]. 实验动物微生物学等级及监测.北京:中国国家标准化管理委员会.
He ZM. 2010. Potentials and prospects: Laboratory animal derived from resource animals in China[J]. Chin J Compar Med, 20(3): 1-7.
[
贺争鸣. 2010. 我国资源动物的实验动物化潜力与展望. 中国比较医学杂志, 20(3): 1-7.]
Hu XL, Li JQ. 2002. Application of Tupaia on studying of virus disease[J]. Virol Sin, 17(3): 276-278.
[
胡溪柳, 李际强. 2002. 树鼩应用于病毒学方面的研究进展. 中国病毒学, 17(3): 276-278.]
Wan XB, Pang QF, Qiu FX, Xu AY, Zhang SQ. 1982. Experimental infection of human rotavirus of adult tree shrew[J]. J Med Res, 3: 2.
[
万新邦, 庞其方, 丘福禧, 胥爱源, 张胜泉. 1982. 人轮状病毒实验感染成年树鼩的研究. 医学研究杂志, 3: 2.]
Wan XB, Pang QF, Qiu FX, Zhang XS, Liu JC, Xv AY, Zhang SQ, Zhang HF, Xu WM..1985.Experimental studies on the susceptibility of adult Tupaia belangeri yunalis to human rotavirus[J].Chin J Microbiol Immunol,5:304-307.[万新邦, 庞其方, 丘福禧, 张新生, 刘玑昌, 胥爱源, 张胜泉, 张惠芬, 徐维民.1985.成年的中国云南树鼩对人轮状病毒易感性的实验研究. 中华微生物学和免疫学杂志, 5:304-307.
Xu XP, Chen HB, Ben KL. 2005. Application of tree shrew as an animal model in biomedical research[J]. Acta Lab Anim Sci Sin, 13(3): 187-190.
[
徐新平, 陈红波, 贲昆龙. 2005. 树鼩在医学生物学中的应用. 中国实验动物学报, 13(3): 187-190.]


 
[1] Xiao-Yan HUANG, Ming-Li LI, Juan XU, Yue-Dong GAO, Wen-Guang WANG, An-Guo YIN, Xiao-Fei LI, Xiao-Mei SUN, Xue-Shan XIA, Jie-Jie DAI. Analysis of the molecular characteristics and cloning of full-length coding sequence of Interleukin-2 in tree shrews [J]. Zoological Research, 2013, 34(2): 121-126.
[2] WANG Wen-Guang, HUANG Xiao-Yan, XU Juan, SUN Xiao-Mei, DAI Jie-Jie, LI Qi-Han. Experimental studies on infant Tupaia belangeri chineses with EV71 infection [J]. Zoological Research, 2012, 33(1): 7-13.
[3] ZHAO Yan,JI Xiang-Shan,ZENG Yong-Qing,DING Lei,YANG Ping-Ping,WANG Hui. Isolation of microsatellite markers for Lateolabrax japonicus and polymorphic analysis [J]. Zoological Research, 2011, 32(5): 515-520.
[4] LI Yuan,SU Jian-Jia,YANG Chun,CAO Ji,OU Chao,LIANG Liang,YANG Fang,WANG. Progress on establishment of tree shrew(Tupaia) chronic infection with HBV in vivo [J]. Zoological Research, 2011, 32(1): 104-108.
[5] HAN Jian-Bao,ZHANG Gao-Hong,DUAN Yong,MA Jian-Ping,ZHANG Xi-He,LUO Rong-Hua,Lü. Sero-epidemiology of six viruses natural infection in Tupaia belangeri chinensis [J]. Zoological Research, 2011, 32(1): 11-16.
[6] MA Xu-Tong,LI Fu-Lin,JIANG Hong-Jun,LI Wen-Hui,ZHANG Yun,DU Ting-Yi. Detection and comparison of physiological indexes in the wild and laboratory tree shrew [J]. Zoological Research, 2011, 32(1): 4-10.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] HAI Sa,LI Jia-le,FENG Jian-bin,Mulati. Systematics of Perca Species Based on Multivariate Morphometrics and Mitochondrial Cytochrome b Gene Variation Analysis[J]. Zoological Research, 2008, 29(2): 113 -120 .
[2] LI Hui,HONG Yong-mi,ZOU Fa-sheng,ZHANG Qiang,HUANG Jun-hui. Bird Diversity and Seasonality in Urban Parks of Guangzhou[J]. Zoological Research, 2008, 29(2): 203 -211 .
[3] LIU Li,GUO Xian-guang,WANG Yue-zhao. Genetic Variation and Diversity of Phrynocephalus vlangalii hongyuanensis in Zoige Wetland Inferred from ND4-tRNAleu Gene[J]. Zoological Research, 2008, 29(2): 121 -126 .
[4] LIU Yan,NIE Wen-hui,HUANG Ling,WANG Jin-huan,SU Wei-ting,LIN Chyi Chyang,Y.. Cloning, Characterization, and FISH Mapping of Four Satellite DNAs from Black Muntjac (Muntiacus crinifrons) and Fea’s Muntjac (M. feae)[J]. Zoological Research, 2008, 29(3): 225 -235 .
[5] DING Zhi-feng,YUAN Ling,ZHU Cheng-lin,HUANG Jin-wen,ZHENG Xi-qu,GUO Xiang-pin. Nesting Characteristics of Yellow-bellied Prinia, Prinia flaviventris[J]. Zoological Research, 2008, 29(3): 270 -276 .
[6] XIONG Jiang,LIU Qiang. A New Chinese Record of Pentatomidae and A Complementary Description of Fernelius indicus Distant (Heteroptera)[J]. Zoological Research, 1996, 17(3): 306 -314 .
[7] LIU Jing-yuan,DU Hong,TIAN Geng-bai,YU Pin-hong,WANG Shen-wen,PEN Hong. Community Structure and Diversity Distributions of Small Mammals in Different Sample Plots in the Eastern Part of Wuling Mountains[J]. Zoological Research, 2008, 29(6): 637 -645 .
[8] XIANG Yu-yong,YANG Mao-fa,LI Zi-zhong. Sex Pheromone Components of the Female Black Cutworm Moth in China: Identification and Field Trials[J]. Zoological Research, 2009, 30(1): 59 -64 .
[9] Marian Plotkin,Stanislav Volynchik,Zahava Barkay,David J. Bergman. Micromorphology and Maturation of the Yellow Granules in the Hornet Gastral Cuticle[J]. Zoological Research, 2009, 30(1): 65 -73 .
[10] HUO Jin-long,HUO Hai-long,MIAO Yong-wang,LI Fu-quan,LIU Li-xian,WU Ge-min,OUYAN. Genetic Diversity of 76 STR Loci in the Dahe Pig[J]. Zoological Research, 2009, 30(1): 105 -108 .