Please wait a minute...
ZOOLOGICAL RESEARCH    2010, Vol. 31 Issue (5) : 561-564     DOI: 10.3724/SP.J.1141.2010.05561
Short report |
Rates and patterns of microsatellite mutations in common carp (Cyprinus carpio L.)
ZHANG Yan 1,2, LU Cui-Yun 2, CAO Ding-Chen 2, XU Peng 1, WANG Shu 1, LI Heng-De 1, ZHAO Zi-Xia 1 , SUN Xiao-Wen 1,2,*
1. The Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China; 2. Heilongjiang Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin 150040, China
Download: PDF(226 KB)  
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks    
During genotyping 150 microsatellites in a F1 family of common carp, six mutations were found at five microsatellite loci. The overall mutation rate of common carp microsatellites was 2.53×10-4 per locus per generation. At five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. The data on mutation rates and patterns can facilitate population genetics studies, and provide useful parameters for estimating a long-term effective population size of common carp.
Keywords Mutation rate      Mutation pattern      Common carp     
PACS:  Q959.468  
Corresponding Authors: SUN Xiao-Wen   
E-mail this article
E-mail Alert
Articles by authors
Cite this article:   
ZHANG Yan,LU Cui-Yun,CAO Ding-Chen,XU Peng,WANG Shu,LI Heng-De,ZHAO Zi-Xia. Rates and patterns of microsatellite mutations in common carp (Cyprinus carpio L.). ZOOLOGICAL RESEARCH, 2010, 31(5): 561-564.
URL:     OR
Balloux F, Lugon-Moulin N. 2002. The estimation of population differentiation with microsatellite markers[J]. Mol Ecol, 11:155-165.
Beacham TD, Supernault J, Miller KM. 2008. Population structure of Dungeness crab (Cancer magister) in British Columbia[J]. J Shellfish Res, 27: 901-906.
Beerli P, Felsenstein J. 2001 Maximum likelihood estimation of a migration matrix and effective population sizes in subpopulations by using a coalescent approach [J]. Proc Natl Acad Sci USA, 98: 4563-4568.
Chakraborty R, Kimmel M, Stivers DN, Davison LJ, Deka R. 1997. Relative mutation rates at di-, tri-, and tetranucleotide microsatellite loci [J]. Proc Natl Acad Sci USA, 94: 1041-1046.
Crooijmans RPMA, Van Der Poel JJ, Groenen MAM, Bierbooms VAF, Komen J. 1997. Microsatellite markers in common carp (Cyprinus carpio L.) [J]. Anim Genet, 28: 129-134.
David L, Jinggui F, Palanisamy R, Hillel J, Lavi U. 2001. Polymorphism in ornamental and common carp strains (Cyprinus carpio L.) as revealed by AFLP analysis and a new set of microsatellite markers [J]. Mol Genet Genom, 266: 353-362.
Ellegren H. 2004. Microsatellites: Simple sequences with complex evolution [J]. Nat Rev Genet, 5: 435-445.
Goldstein DB, Schlotterer C. 1999. Microsatellites: Evolution and Applications [M]. Oxford Jones: Oxford University Press.
FAO. 2002. Yearbook of fisheries statistics summary tables[EB/OL].
Jones AG, Rosenqvist G, Berglund A, Avise JC. 1999. Clustered microsatellite mutations in the pipefish Syngnathus typhle
[J]. Genetics, 152: 1057-1063.
Laloi D, Richard M, Lecomte J, Massot M, Clobert J. 2004. Multiple paternity in clutches of common lizard Lacerta vivipara: data from microsatellite markers[J]. Mol Ecol, 13: 719-723.
Lehmann T, Hawley WA, Grebert H, Collins FH. 1998. The effective population size of Anopheles gambiae in Kenya: Implications for population structure [J]. Mol Biol Evol, 15: 264-276
MacKiewicz M, Fletcher DE, Wilkins SD, DeWoody JA, Avise JC. 2002. A genetic assessment of parentage in a natural population of dollar sunfish (Lepomis marginatus) based on microsatellite markers [J]. Mol Ecol, 11: 1877-1883.
Nei M. 1987. Molecular Evolutionary Genetics [M]. New York: Columbia Univ Press.
Naylor RL, Goldburg RJ, Primavera JH, Kautsky N, Beveridge MC, Clay J, Folke C, Lubchenco J, Mooney H, Troell M. 2000. Effect of aquaculture on world fish supplies [J]. Nature, 405: 1017-1024.
Ohta T, Kimura M. 2007. A model of mutation appropriate to estimate the number of electrophoretically detectable alleles in a finite population[J]. Genet Res, 89: 367-370.
Ohno S. 1970. Evolution by Gene Duplication [M]. London: Allen and Unwin.
Rohlf RJ, Sokal RR. 1995. Statistical Tables [M]. New York: W.H. Freeman and Company.
Schlotterer C. 2000. Evolutionary dynamics of microsatellite DNA [J]. Chromosoma, 109: 365-371.
Steinberg EK, Lindner KR, Gallea J, Maxwell A, Meng J, Allendorf FW. 2002. Rates and patterns of microsatellite mutations in pink salmon [J]. Mol Biol Evol, 19: 1198-1202.
Sun XW, Liang LQ. 2004. A genetic linkage map of common carp (Cyprinus earpio L.) and mapping of a locus associated with cold tolerance [J]. Aquaculture, 238: 165-172.
Vitalis R, Couvet D. 2001. Estimation of effective population size and migration rate from one- and two-locus identity measures
[J]. Genetics, 157: 911-925.
Weber JL, Wong, C. 1993. Mutation of human short tandem repeats [J]. Hum Mol Genet, 2: 1123-1128.
Wei DW, Lou YD, Sun XW, Shen JB. 2001. Isolation of microsatellite markers in the common carp (Cyprinus carpio)
[J]. Zool Res, 22: 238-241. (in Chinese)
Xia J, He X, Yu Z. 2009. Isolation and characterization of fourteen novel microsatellite loci in the Hong Kong oyster, Crassostrea hongkongensis [J]. J Conserv Genet, 10: 1572-9737 (Online).
Yue, GH, David L, Orban L. 2007. Mutation rate and pattern of microsatellites in common carp (Cyprinus carpio L.) [J]. Genetica, 129: 329-331.
Zhang Y, Liang LQ, Chang YM, Hou N, Lu CY, Sun XW. 2007. Mapping and genetic effect analysis of quantitative trait loci related to body size in common carp (Cyprinus carpio L.) [J]. Hereditas : Beijing, 29: 1243-1248. (in Chinese)
Zhang Y, Liang L, Jiang P, Li D, Lu C, Sun X. 2008. Genome evolution trend of common carp (Cyprinus carpio L.) as revealed by the analysis of microsatellite loci in a gynogentic family [J]. J Genet Genomics, 35: 97-103.

[1] Ian S. LOGAN. ZIKA-How fast does this virus mutate?[J]. ZOOLOGICAL RESEARCH, 2016, 37(2): 110-115.
[2] CHANG Yu-Mei,GAO Guo-Qiang,XU Li-Hua,LIU Jin-Liang,LIU Chun-Lei,Liang Li-qun. Correlation and location of EST markers with cold tolerance trait of common carp (Cyprinus carpio L.)[J]. ZOOLOGICAL RESEARCH, 2010, 31(6): 581-586.
[3] QUAN Ying-chun,SUN Xiao-wen,LIANG Li-qun. Microsatellite Variation Among Four Breeding Populations of Common Carps[J]. ZOOLOGICAL RESEARCH, 2005, 26(6): 595-602.
[4] XIAO Dong,CHEN Song-lin,LIN Hao-ran. Studies on Enhancement Growth Additive of Microca psule Type Recombinant Common Carp Growth Hormone[J]. ZOOLOGICAL RESEARCH, 2002, 23(6): 449-455.
[5] WEI Dong-Wang,LOU Yun-Dong,SUN Xiao-Wen,SHEN Jun-Bao. Isolation of Microsatellite Markers in the Common Carp (Cyprinus carpio)[J]. ZOOLOGICAL RESEARCH, 2001, 22(3): 238-241.
[6] WANG Li,LIN Hao-ran. Distributions and Variations of sGnRH in Discrete Brain Areas From Common Carp (Cyprinus carpio L.) of Different Ages and Gonadal Conditions[J]. ZOOLOGICAL RESEARCH, 1998, 19(3): 197-202.
[7] WANG Li,LIN Hao-ran,ZHANG Wei-min. Effects of Different Hypothalamic Peptides and Neurotransmitters on Gonadotropin and Growth Hormone Secretion in Common Carp (Cyprinus carpio L.)[J]. ZOOLOGICAL RESEARCH, 1997, 18(1): 79-84.
Full text