Zoological Research ›› 2019, Vol. 40 ›› Issue (3): 231-235.doi: 10.24272/j.issn.2095-8137.2019.038

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Threshold for maximal electroshock seizures (MEST) at three developmental stages in young mice

Cheng Xiang1,Zhi-Na Li1,Tian-Zhuang Huang1,Jing-Hui Li2,Lei Yang3,Jing-Kuan Wei1()   

  1. 1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China
    2. First Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650032, China
    3. Department of Neurosurgery, Kunming Children's Hospital, Kunming Yunnan 650021, China
  • Received:2018-08-30 Accepted:2019-01-29 Online:2019-05-18 Published:2019-03-28
  • Contact: Jing-Kuan Wei E-mail:weijingkuan@163.com
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (81403191) and Yunnan Provincial Natural Science Foundation (2018FB118, 2015FA004, and KKSY201626001)

Abstract:

Early brain development after birth is extremely dynamic, suggesting that potential functional changes occur during this period. In this study, the maximal electroshock seizure threshold (MEST) was used to explore the electrophysiological variation among three developmental stages in young mice (no more than 5 weeks old). The induced electroshock seizure (ES) behavior of early postnatal mice (1–2-weeks old) differed from that during weaning (3 weeks old) and early puberty (4–5-weeks old). Thus, we further explored their respective characteristic responses to the ES parameters. When the stimulation current (SC) was limited to 4.0 mA, only the 1–2-week-old mice were induced to exhibit ES behavior at voltages of 30 V and 40 V, indicating they were more sensitive to maximal electroshock seizure (MES) (response to lower voltage). Surprisingly, however, they showed substantially lower mortality than the older groups under higher voltage conditions (60, 100, 160, and 200 V), suggesting better tolerance to the SC. We also found that when the current limit decreased to 3.5 mA, the 4–5-week-olds mice exhibited stable ES behavior with low mortality, while for 3-week-olds mice, the SC limit required to be reduced to 1.5 mA. In conclusion, our findings showed that neural sensitivity to MES was significantly different in young mice before puberty. Thus, greater attention should be given to distinguishing the developmental period of mice, especially in electrophysiological examination.

Key words: Brain, Young mice, Neurophysiology, Maximal electroshock seizure, Epilepsy

"

Current limited to 4.0 mA Current limited to 3.5 mA Current limited to 1.5 mA Total (n)
1–2 weeks 85 10 10 105
3 weeks 36 25 52 113
4–5 weeks 39 70 23 132
Total (n) 160 105 85 350

Figure 1

Electroshock seizure (ES) behavior at different developmental stages in mice"

Figure 2

Maximal electroshock seizure (MES) occurrence, no-response, and death voltage range of 1–2-week-old, 3-week-old, and 4–5-week-old mice at 4.0 mA "

"

Age Number (n) ES Death Chi-square test
1–2 weeks 63 100% 0 P<0.000 1
3–5 weeks 46 4.3% 95.6%

Figure 3

Voltage range of 3-week-old and 4–5-week-old mice showing successful maximal electroshock seizure (MES), no-response, and death with current limited to 3.5 mA or 1.5 mA "

"

Group Treatment Total (MES/Anticonvulsant) Inhibition (%)
1–2 weeks ES 9 (9/0) 62.5 (P #=0.008 8)
ES+PB 16(6/10)
3 weeks ES 12(12/0)

53.8

(P=0.005 2)

ES+PB 13(6/7)
4–5 weeks ES 16(16/0)

50

(P=0.006 6)

ES+PB 8(4/4)
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