Zoological Research ›› 2019, Vol. 40 ›› Issue (2): 102-107.doi: 10.24272/j.issn.2095-8137.2018.064

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Developmental expression of three prmt genes in Xenopus

Cheng-Dong Wang1,#, Xiao-Fang Guo2,5,#, Thomas Chi Bun Wong1, Hui Wang1, Xu-Feng Qi2,5, Dong-Qing Cai2,5, Yi Deng3, Hui Zhao1,4,*   

  1. 1 Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
    2 Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou Guangdong 510632, China
    3 Guangdong Provincial Key Laboratory of Cell Microenvironment, Department of Biology, South University of Science and Technology of China, Shenzhen Guangdong 518055, China
    4 Kunming Institute of Zoology, Chinese Academy of Sciences-The Chinese University of Hong Kong Joint Laboratory of Bioresources and Molecular Research of Common Diseases, Hong Kong SAR, China
    5 Department of Developmental & Regenerative Biology, College of Life Science and Technology, Jinan University, Guangzhou Guangdong 510632, China
  • Online:2019-03-18 Published:2019-01-11
  • Contact: Hui Zhao,E-mail:zhaohui@cuhk.edu.hk E-mail:zhaohui@cuhk.edu.hk
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Abstract:

Protein arginine methyltransferases (PRMTs) are involved in many cellular processes via the arginine methylation of histone or non-histone proteins. We examined the expression patterns of prmt4, prmt7, and prmt9 during embryogenesis in Xenopus using whole-mount in situ hybridization and quantitative reverse transcription polymerase chain reaction (RT-PCR). Xenopus prmt4 and prmt7 were expressed in the neural crest, brain, and spinal cord, and also detected in the eye, branchial arches, and heart at the tailbud stage. Specific prmt9 signals were not detected in Xenopus embryos until the late tailbud stage when weak expression was observed in the branchial arches. Quantitative RT-PCR indicated that the expressions of prmt4 and prmt7 were up-regulated during the neurula stage, whereas prmt9 maintained its low expression until the late tailbud stage, consistent with the whole-mount in situ hybridization results. Thus, the developmental expression patterns of these three prmt genes in Xenopus embryos provide a basis for further functional study of such genes.

Key words: Xenopus, prmt, Arginine methylation, Gene expression pattern 

CLC Number: 

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