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Zoological Research    0, Vol. Issue () : 64-     DOI: 10.24272/j.issn.2095-8137.2017.012
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 In vivo genome editing thrives with diversified CRISPR technologies
Xun Ma1, Avery Sum-Yu Wong1, Hei-Yin Tam1, Samuel Yung-Kin Tsui1, Dittman Lai-Shun Chung1, Bo Feng1,2,*
1 CUHK-GIBH CAS Joint Research Laboratory on Stem Cell and Regenerative Medicine, Key Laboratory for Regenerative Medicine in Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
2 SBS Core Laboratory, CUHK Shenzhen Research Institute, Shenzhen Guangdong 518057, China
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Abstract  Prokaryotic type II adaptive immune systems have been developed into the versatile CRISPR technology, which has been widely applied in site-specific genome editing and has revolutionized biomedical research due to its superior efficiency and flexibility. Recent studies have greatly diversified CRISPR technologies by coupling it with various DNA repair mechanisms and targeting strategies. These new advances have significantly expanded the generation of genetically modified animal models, either by including species in which targeted genetic modification could not be achieved previously, or through introducing complex genetic modifications that take multiple steps and cost years to achieve using traditional methods. Herein, we review the recent developments and applications of CRISPR-based technology in generating various animal models, and discuss the everlasting impact of this new progress on biomedical research.
Keywords CRISPR/Cas9      Genome editing      Animal models     
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Xun Ma, Avery Sum-Yu Wong, Hei-Yin Tam, Samuel Yung-Kin Tsui, Dittman Lai-Shun Chung, Bo Feng.  In vivo genome editing thrives with diversified CRISPR technologies. Zoological Research, 10.24272/j.issn.2095-8137.2017.012
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http://www.zoores.ac.cn/EN/10.24272/j.issn.2095-8137.2017.012     OR     http://www.zoores.ac.cn/EN/Y0/V/I/64
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