Zoological Research ›› 2016, Vol. 37 ›› Issue (2): 96-102.doi: 10.13918/j.issn.2095-8137.2016.2.96

Special Issue: Fish biology Genetics & evolution Immunology

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Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis

Qiang CHEN1,2, Xin-Jiang LU1, Ming-Yun LI1, Jiong CHEN1,2   

  1. 1 Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
    2 Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China
  • Received:2015-10-22 Revised:2015-12-11 Online:2016-03-18 Published:2016-03-18
  • Contact: Jiong CHEN E-mail:jchen1975@163.com
  • Supported by:

    This project was supported by the Program for the National Natural Science Foundation of China (31372555), Zhejiang Provincial Natural Science Foundation of China (LZ13C190001), Scientific Research Foundation of Graduate School of Ningbo University (G15063), and KC Wong Magna Fund in Ningbo University

Abstract: Colony-stimulating factor 1 receptor (CSF-1R) is an important regulator of monocytes/macrophages (MO/MΦ). Although several CSF-1R genes have been identified in teleosts, the precise role of CSF- 1R in ayu (Plecoglossus altivelis) remains unclear. In this study, we characterized the CSF-1R homologue from P. altivelis, and named it PaCSF-1R. Multiple sequence alignment and phylogenetic tree analysis showed that PaCSF-1R was most closely related to that of Japanese ricefish (Oryzias latipes). Tissue distribution and expression analysis showed that the PaCSF-1R transcript was mainly expressed in the head kidney-derived MO/MΦ, spleen, and head kidney, and its expression was significantly altered in various tissues upon Vibrio anguillarum infection. After PaCSF-1R neutralization for 48 h, the phagocytic activity of MO/MΦ was significantly decreased, suggesting that PaCSF-1R plays a role in regulating the phagocytic function of ayu MO/MΦ.