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陈朝豪, 李碧君, 谷小慧, 林浩然, 夏军红. 2019: 吉富YY超雄罗非鱼分子标记辅助选育. 动物学研究, 40(2): 108-112. DOI: 10.24272/j.issn.2095-8137.2018.071
引用本文: 陈朝豪, 李碧君, 谷小慧, 林浩然, 夏军红. 2019: 吉富YY超雄罗非鱼分子标记辅助选育. 动物学研究, 40(2): 108-112. DOI: 10.24272/j.issn.2095-8137.2018.071
Chao-Hao Chen, Bi-Jun Li, Xiao-Hui Gu, Hao-Ran Lin, Jun-Hong Xia. 2019: Marker-assisted selection of YY supermales from a genetically improved farmed tilapia-derived strain. Zoological Research, 40(2): 108-112. DOI: 10.24272/j.issn.2095-8137.2018.071
Citation: Chao-Hao Chen, Bi-Jun Li, Xiao-Hui Gu, Hao-Ran Lin, Jun-Hong Xia. 2019: Marker-assisted selection of YY supermales from a genetically improved farmed tilapia-derived strain. Zoological Research, 40(2): 108-112. DOI: 10.24272/j.issn.2095-8137.2018.071

吉富YY超雄罗非鱼分子标记辅助选育

Marker-assisted selection of YY supermales from a genetically improved farmed tilapia-derived strain

  • 摘要: 世界罗非鱼养殖品系主要是吉富(GIFT)及其来源的品系。GIFT罗非鱼雄性比雌性生长快。利用雄性激素(17 α methyltestosterone)将雌性个体性逆转为表型雄性个体是罗非鱼全雄苗种生产的主要方式。在苗种生产过程中大量使用性激素可能对工人的健康造成影响,并对环境生态造成威胁。因此,不需要或直接使用激素生产全雄罗非鱼苗种的生产方式具有很大的市场前景。本研究通过鉴定性连锁分子标记,并应用标记辅助育种技术从一个GIFT来源的罗非鱼品系中选育出了24尾XY伪雌鱼及431尾YY超雄罗非鱼。进一步对YY超雄个体与雌性个体杂交产生的三个子一代群体的生产性能进行检测发现子代雄性率分别为94.1%、99.5%及99.6%。前三个月日增重达到1.4 g (n=997),体重分布均匀,达到生产的要求。研究显示我们已建立了一套高效吉富YY超雄罗非鱼分子标记辅助选育技术体系。选育的YY超雄罗非鱼将有助于减少罗非鱼全雄苗种生产过程中激素的使用。

     

    Abstract: Genetically improved farmed tilapia (GIFT) and GIFT-derived strains account for the majority of farmed tilapia worldwide. As male tilapias grow much faster than females, they are often considered more desirable in the aquacultural industry. Sex reversal of females to males using the male sex hormone 17-α-methyltestosterone (MT) is generally used to induce phenotypic males during large-scale production of all male fingerlings. However, the widespread use of large quantities of sex reversal hormone in hatcheries may pose a health risk to workers and ecological threats to surrounding environments. Breeding procedures to produce genetically all-male tilapia with limited or no use of sex hormones are therefore urgently needed. In this study, by applying marker-assisted selection (MAS) for the selection of YY supermales from a GIFT-derived strain, we identified 24 XY pseudofemale and 431 YY supermale tilapias. Further performance evaluation on the progenies of the YY supermales resulted in male rates of 94.1%, 99.5% and 99.6%, respectively, in three populations, and a daily increase in body weight of 1.4 g at 3 months (n=997). Our study established a highly effective MAS procedure in the selection of YY supermales from a GIFT-derived strain. Furthermore, the development of MAS-selected YY supermales will help reduce the utilization of hormones for controlling sex in the tilapia aquaculture.

     

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