Abstract: Hematologic and hemachemical parameters in Rhesus monkey (Macaca mulatta),Pig-tailed monkey (Macaca nemestrina) and Assamense monkey (Macaca assamensis) were studied in the present paper.The blood parameters of above three species were similar except for amount of white blood cell,the difference of that was related to the length of keeping and feed condition.Amylase and γ-glutamyl transpeptidase in serum of rhesus monkey were quite higher than that of human being.Other hemachemical parameters were similar to the humans normal parameter.The percent among five bands of the Lactic dehydrogenase isoenzyme was different from that of normal human.The phosphocreatine kinase isoenzyme of healthy human showed one band,however,that of the rhesus monkey showed two bands,the amount of the MM band and MB band in rhesus monkey was about 70% and 30% respectively.